LOCUS       sequence-334383-        9383 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector to co-express a human CDH1 spsgRNA (sg2-Cdh1) with
            NLS-mScarlet-I.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9383)
  AUTHORS   Wang S, Matsumoto K, Lish SR, Cartagena-Rivera AX, Yamada KM
  TITLE     Budding epithelial morphogenesis driven by cell-matrix versus 
            cell-cell adhesion.
  JOURNAL   Cell. 2021 Jul 8;184(14):3702-3716.e30. doi: 
            10.1016/j.cell.2021.05.015. Epub 2021 Jun 15.
  PUBMED    34133940
REFERENCE   2  (bases 1 to 9383)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.05.015"; journalName: "Cell"; date: "2021-07-8-
            8"; volume: "184"; issue: "14"; pages: "3702-3716.e30"
COMMENT     Sequence Label: 
            pW266-lenti-spsgRNA-hsCDH1-sg2-pEF1s-NLS-mScarlet-I-P2A-BlastR
FEATURES             Location/Qualifiers
     source          1..9383
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(120..140)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        125..454
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      305..440
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     367..386
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        502..549
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             568..942
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1072..1193
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1109..1128)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1163..1182
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1242..1258)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1242..1258)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1255..1277)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1266..1282
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1290..1296,1297..1314,1315..1320))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1335..1356
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1473..1490)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1644..2232)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1724..1743)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2403..3194,3195..3263))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3026..3045
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3264..3368)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3443..3462)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3634..4013
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4015..4213
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4168..4188
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4231..4411
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4458..4583
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5076..5309
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5494..5538
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5836..5953
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6004..6252
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     6004..6024
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     6175..6194
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        6274..6349
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        6411..6622
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             6650..6697
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6698..7390
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="VSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGGA
                     VTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRHS
                     TGGMDELYK"
     primer_bind     complement(6828..6846)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7096..7115
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             7400..7456
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7457..7855
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    7871..8459
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7924..7944)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8342..8353)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8531..8764
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8790..8807)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8796..9020
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(9066..9383,1..111)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9153..9172)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(9363..9383,1)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
       61 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg
      121 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
      181 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
      241 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
      301 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
      361 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
      421 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
      481 tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta
      541 taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc
      601 accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg
      661 gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc
      721 gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg
      781 gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg
      841 ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg
      901 gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct acgagatttc
      961 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     1021 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
     1081 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     1141 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     1201 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     1261 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     1321 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     1381 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     1441 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     1501 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     1561 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     1621 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     1681 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     1741 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     1801 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     1861 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     1921 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     1981 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     2041 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     2101 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     2161 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     2221 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     2281 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     2341 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     2401 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     2461 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     2521 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     2581 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     2641 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     2701 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     2761 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     2821 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     2881 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     2941 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     3001 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     3061 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     3121 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     3181 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     3241 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     3301 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     3361 gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg
     3421 atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc
     3481 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa
     3541 caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc
     3601 tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact agttattaat
     3661 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
     3721 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     3781 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     3841 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     3901 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     3961 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
     4021 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
     4081 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
     4141 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
     4201 tctatataag cagcgcgttt tgcctgtact gggtctctct ggttagacca gatctgagcc
     4261 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     4321 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     4381 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
     4441 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
     4501 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     4561 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
     4621 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     4681 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     4741 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     4801 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     4861 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     4921 cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta
     4981 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
     5041 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg
     5101 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa
     5161 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag
     5221 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg
     5281 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt
     5341 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
     5401 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
     5461 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
     5521 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
     5581 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
     5641 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
     5701 ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac
     5761 agatccattc gattagtgaa cggatcggca ctgcgtgcgc caattctgca gacaaatggc
     5821 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
     5881 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
     5941 aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttggt taattaaggt
     6001 accgagggcc tatttcccat gattccttca tatttgcata tacgatacaa ggctgttaga
     6061 gagataatta gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt
     6121 agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat
     6181 catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa
     6241 ggacgaaaca ccgtggccgg ggacgccgag cgagttttag agctagaaat agcaagttaa
     6301 aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct tttttgaatt
     6361 cgctagctag gtcttgaaag gagtgggaat tggctccggt gcccgtcagt gggcagagcg
     6421 cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgat ccggtgccta
     6481 gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc
     6541 cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa
     6601 cgggtttgcc gccagaacac aggaccggtt ctagagcgct gccaccatga agcgacctgc
     6661 cgccacaaag aaggctggac aggctaagaa gaagaaagtg agcaagggcg aggcagtgat
     6721 caaggagttc atgcggttca aggtgcacat ggagggctcc atgaacggcc acgagttcga
     6781 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
     6841 gaccaagggt ggccccctgc ccttctcctg ggacatcctg tcccctcagt tcatgtacgg
     6901 ctccagggcc ttcatcaagc accccgccga catccccgac tactataagc agtccttccc
     6961 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgccg tgaccgtgac
     7021 ccaggacacc tccctggagg acggcaccct gatctacaag gtgaagctcc gcggcaccaa
     7081 cttccctcct gacggccccg taatgcagaa gaagacaatg ggctgggaag cgtccaccga
     7141 gcggttgtac cccgaggacg gcgtgctgaa gggcgacatt aagatggccc tgcgcctgaa
     7201 ggacggcggc cgctacctgg cggacttcaa gaccacctac aaggccaaga agcccgtgca
     7261 gatgcccggc gcctacaacg tcgaccgcaa gttggacatc acctcccaca acgaggacta
     7321 caccgtggtg gaacagtacg aacgctccga gggccgccac tccaccggcg gcatggacga
     7381 gctgtacaag ggatccggcg caacaaactt ctctctgctg aaacaagccg gagatgtcga
     7441 agagaatcct ggaccgatgg ccaagccttt gtctcaagaa gaatccaccc tcattgaaag
     7501 agcaacggct acaatcaaca gcatccccat ctctgaagac tacagcgtcg ccagcgcagc
     7561 tctctctagc gacggccgca tcttcactgg tgtcaatgta tatcatttta ctgggggacc
     7621 ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca acctgacttg
     7681 tatcgtcgcg atcggaaatg agaacagggg catcttgagc ccctgcggac ggtgccgaca
     7741 ggtgcttctc gatctgcatc ctgggatcaa agccatagtg aaggacagtg atggacagcc
     7801 gacggcagtt gggattcgtg aattgctgcc ctctggttat gtgtgggagg gctaaacgcg
     7861 ttaagtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     7921 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     7981 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     8041 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     8101 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     8161 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     8221 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     8281 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     8341 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     8401 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg
     8461 tcgactttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     8521 aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt
     8581 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     8641 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     8701 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     8761 agcagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     8821 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     8881 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     8941 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     9001 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
     9061 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     9121 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     9181 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     9241 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     9301 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     9361 tagtggactc ttgttccaaa ctg
//
