LOCUS       sequence-334387-        9439 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To monitor the status of Necl-5, the plasmid encodes a recombinant 
            Necl-5 fused extracellularly to the mScarlet fluorescent protein and
            intracellularly to the mNeonGreen fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9439)
  AUTHORS   Ichise H, Tsukamoto S, Hirashima T, Konishi Y, Oki C, Tsukiji S, 
            Iwano S, Miyawaki A, Sumiyama K, Terai K, Matsuda M
  TITLE     Functional visualization of NK Cell-mediated killing of metastatic 
            single tumor cells.
  JOURNAL   Elife. 2022 Feb 3;11. pii: 76269. doi: 10.7554/eLife.76269.
  PUBMED    35113018
REFERENCE   2  (bases 1 to 9439)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-02-3"; volume: "11. pii: 76269. doi"; pages: " 
            10.7554/eLife.76269"
COMMENT     Sequence Label: pPBbsr2-Necl-5-ScNeo
FEATURES             Location/Qualifiers
     source          1..9439
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   674..708
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1011..1390
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          1668..2684
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2608..2630
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2692..2711
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             2838..3533
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(2971..2989)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3239..3258
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    5503..6052
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5647..5664)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5874..5893
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(6523..6540)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6529..6753
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6800..6816)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6824..6840
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6848..6854,6855..6872,6873..6878))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6893..6914
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   7148..7210
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7251..7271)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7251..7269)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7290..7306)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7314..7330
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7338..7344,7345..7362,7363..7368))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7383..7404
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7521..7538)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7692..8280)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7772..7791)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8451..9242,9243..9311))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9074..9093
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9312..9416)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggggcgcgc
      661 cattctagat taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat
      721 attgctctct ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg
      781 ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact
      841 ataacgaccg cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta
      901 actcatacga taattatatt gttatttcat gttctactta cgtgataact tattatatat
      961 atattttctt gttatagata tcaactagaa tgctagcatg ggcccatctc gacattgatt
     1021 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     1081 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     1141 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     1201 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     1261 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     1321 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     1381 tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc
     1441 cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg
     1501 gggggggggg gsgcgcgccr gggggggggg gggggggggg ggggsggggs ggggsgrggc
     1561 ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga
     1621 ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcgc
     1681 gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac
     1741 tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt
     1801 agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc
     1861 tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg
     1921 tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg
     1981 cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc
     2041 ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt
     2101 gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag
     2161 ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg
     2221 ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg
     2281 ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg
     2341 cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt
     2401 tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc
     2461 gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt
     2521 cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct
     2581 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
     2641 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
     2701 tggttattgt gctgtctcat cattttggca aagaattggg atccgaattc accatggctc
     2761 aactcgcccg agccacccgc tccccgctgt catggctgct gctgctgttc tgctatgcac
     2821 tccggaaagc gggtgggatg gtgagcaagg gcgaggcagt gatcaaggag ttcatgcggt
     2881 tcaaggtgca catggagggc tccatgaacg gccacgagtt cgagatcgag ggcgagggcg
     2941 agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc
     3001 tgcccttctc ctgggacatc ctgtcccctc agttcatgta cggctccagg gccttcacca
     3061 agcaccccgc cgacatcccc gactactata agcagtcctt ccccgagggc ttcaagtggg
     3121 agcgcgtgat gaacttcgag gacggcggcg ccgtgaccgt gacccaggac acctccctgg
     3181 aggacggcac cctgatctac aaggtgaagc tccgcggcac caacttccct cctgacggcc
     3241 ccgtaatgca gaagaagaca atgggctggg aagcgtccac cgagcggttg taccccgagg
     3301 acggcgtgct gaagggcgac attaagatgg ccctgcgcct gaaggacgga ggccgctacc
     3361 tggcggactt caagaccacc tacaaggcca agaagcccgt gcagatgccc ggcgcctaca
     3421 acgtcgaccg caagttggac atcacctccc acaacgagga ctacaccgtg gtggaacagt
     3481 acgaacgctc cgagggccgc cactccaccg gcggcatgga cgagctgtac aagggaggct
     3541 cggatatacg tgtgctggtg ccctacaatt cgacaggcgt cttgggaggg tcgaccacct
     3601 tgcactgtag tctgacttct aatgagaatg tgactatcac tcaaataacc tggatgaaga
     3661 aggattcagg tggatcccac gctcttgtgg ctgtcttcca ccccaagaag gggcccaaca
     3721 tcaaagagcc agagagggtg aaattcttgg ctgcccaaca ggatctgagg aacgcatctc
     3781 tggccatctc gaacttaagt gtagaagacg aaggcatcta tgaatgtcag attgccacat
     3841 tccccagagg cagtagaagc accaatgcct ggctgaaggt gcaagcccga cctaagaaca
     3901 ctgcagaggc cctggagccc tctcccacct tgatactgca ggatgtggct aaatgcatct
     3961 ctgccaatgg tcaccctcct ggacgaatct cttggccctc gaatgtgaat ggaagtcacc
     4021 gtgaaatgaa ggaaccaggg tcccagccgg gcaccaccac agttaccagc tacctctcca
     4081 tggtaccttc tcgccaggca gacggcaaga acatcacctg cacggtggag catgaaagct
     4141 tacaggagct ggaccagctg ctggtgaccc tttcccaacc ctatccacct gaaaacgtgt
     4201 ccatctctgg ctatgacggc aactggtatg ttggcctcac taacttgacc ctgacctgtg
     4261 aagctcacag caaaccagcg cctgacatgg ctggatataa ctggagcacg aacacgggtg
     4321 actttcccaa ctctgttaag cgccagggca atatgcttct aatctccacc gtagaggatg
     4381 gtctcaataa cacggtcatt gtgtgcgaag tcaccaatgc cctagggtct gggcagggcc
     4441 aagtgcacat cattgttaaa gagaaacctg agaatatgca gcaaaataca agattacacc
     4501 taggctacat ctttcttatc gtctttgtcc tcgctgtagt catcatcatc gcagcactat
     4561 acactatacg aagatgcagg catggtcgtg ctctgcagtc caatccctca gagagggaga
     4621 acgtccagta ttcatctgtg aacggcgact gtagactgaa catggagcca aacagcacaa
     4681 ggttcgaaat ggtaagcaag ggggaggaag ataatatggc ttcccttcca gcaacgcacg
     4741 aattgcatat ctttggaagt attaatggag ttgatttcga catggtgggg cagggtacgg
     4801 gaaacccaaa cgatggctat gaagaactta acctcaagtc taccaaaggc gatctccaat
     4861 tctcaccctg gatattggtc ccacacatcg gctacgggtt ccatcaatac ctgccttatc
     4921 ctgacgggat gagtcctttt caagctgcta tggttgacgg aagtggctac caagttcacc
     4981 gcacaatgca atttgaggat ggtgcatctc ttacggttaa ttaccgatat acatatgagg
     5041 gttcacacat caagggggaa gctcaggtca agggcactgg atttcccgca gatggcccag
     5101 ttatgaccaa ttctctgacc gcagccgatt ggtgtcgaag caagaagacc taccctaacg
     5161 acaagactat catctcaacc ttcaaatggt cctacaccac aggtaacgga aagcggtata
     5221 ggtctacagc tcggacaaca tatacctttg cgaagcctat ggccgctaac tatttgaaaa
     5281 accaaccgat gtatgttttt cggaagaccg aattgaaaca ttcaaaaacg gagttgaatt
     5341 tcaaagagtg gcagaaggca ttcaccgacg taatgggtat ggatgagttg tataaataag
     5401 cggcggccgc tctagagtcg acgggccgcg gtaacaattg ttaactaact taagctagca
     5461 acggtttccc tctagcggga tcaattccgc cccccccccc taacgttact ggccgaagcc
     5521 gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt
     5581 ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc
     5641 tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc
     5701 tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc
     5761 cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg
     5821 cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct
     5881 cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat
     5941 ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc
     6001 taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgataa taccatggtc
     6061 atgaaaacat ttaacatttc tcaacaagat ctagaattag tagaagtagc gacagagaag
     6121 attacaatgc tttatgagga taataaacat catgtgggag cggcaattcg tacgaaaaca
     6181 ggagaaatca tttcggcagt acatattgaa gcgtatatag gacgagtaac tgtttgtgca
     6241 gaagccattg cgattggtag tgcagtttcg aatggacaaa aggattttga cacgattgta
     6301 gctgttagac acccttattc tgacgaagta gatagaagta ttcgagtggt aagtccttgt
     6361 ggtatgtgta gggagttgat ttcagactat gcaccagatt gttttgtgtt aatagaaatg
     6421 aatggcaagt tagtcaaaac tacgattgaa gaactcattc cactcaaata tacccgaaat
     6481 taaaagtcga gatgcatcga tgatctagag ctcgctgatc agcctcgact gtgccttcta
     6541 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     6601 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     6661 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     6721 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga acctgcagcc
     6781 caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     6841 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     6901 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     6961 gccagcggat cttaaaagtt ttgttacttt atagaagaaa ttttgagttt ttgttttttt
     7021 ttaataaata aataaacata aataaattgt ttgttgaatt tattattagt atgtaagtgt
     7081 aaatataata aaacttaata tctattcaaa ttaataaata aacctcgata tacagaccga
     7141 taaaacacat gcgtcaattt tacgcatgat tatctttaac gtacgtcaca atatgattat
     7201 ctttctaggg ttaatctagt atacgcgttt aattaatcca gcttttgttc cctttagtga
     7261 gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     7321 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     7381 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     7441 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     7501 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     7561 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     7621 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     7681 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     7741 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     7801 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     7861 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     7921 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     7981 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     8041 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     8101 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     8161 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     8221 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     8281 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     8341 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     8401 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     8461 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     8521 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     8581 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     8641 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     8701 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     8761 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     8821 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     8881 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     8941 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     9001 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     9061 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     9121 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     9181 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     9241 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     9301 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     9361 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     9421 tttccccgaa aagtgccac
//
