LOCUS       sequence-33439-e         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Samara NL, Datta AB, Berndsen CE, Zhang X, Yao T, Cohen RE, 
            Wolberger C
  TITLE     Structural insights into the assembly and function of the SAGA 
            deubiquitinating module.
  JOURNAL   Science. 2010 May 21. 328(5981):1025-9.
  PUBMED    20395473
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2010 May 21. 328(5981):1025-9."
COMMENT     Sequence Label: Ubp8/PET32a
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..373
                     /codon_start=1
                     /gene="trxA"
                     /product="E. coli thioredoxin"
                     /label=TrxA
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDE
                     IADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFL
                     DANLA"
     CDS             395..412
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             422..439
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             446..490
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             506..520
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             563..583
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 gaaattcctc tgaatatttt gtttacttta agaaggagat atacatatga gcgataaaat
       61 tattcacctg actgacgaca gttttgacac ggatgtactc aaagcggacg gggcgatcct
      121 cgtcgatttc tgggcagagt ggtgcggtcc gtgcaaaatg atcgccccga ttctggatga
      181 aatcgctgac gaatatcagg gcaaactgac cgttgcaaaa ctgaacatcg atcaaaaccc
      241 tggcactgcg ccgaaatatg gcatccgtgg tatcccgact ctgctgctgt tcaaaaacgg
      301 tgaagtggcg gcaaccaaag tgggtgcact gtctaaaggt cagttgaaag agttcctcga
      361 cgctaacctg gccggttctg gttctggcca tatgcaccat catcatcatc attcttctgg
      421 tctggtgcca cgcggttctg gtatgaaaga aaccgctgct gctaaattcg aacgccagca
      481 catggacagc ccagatctgg gtaccgacga cgacgacaag gccatggctg atatcggatc
      541 cgaattcgag ctccgtcgac aagaaaacct gtattttcag ggagctgcag ctgcaatgag
      601 catttgtcca catatacagc aagtatttca gaatgaaaag tctaaagatg gggttctaaa
      661 aacgtgcaat gctgccaggt atatattaaa tcattccgta cccaaggaaa aattcttaaa
      721 caccatgaaa tgtggtacat gccacgaaat aaactctggt gcaactttca tgtgtctaca
      781 atgtggattt tgtggatgtt ggaaccattc gcattttctc tctcacagta aacagattgg
      841 tcacatattt ggtatcaact caaataatgg ccttttattt tgcttcaatg tgaggact
//
