LOCUS       sequence-334390-        7856 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encoding mouse Gnaq.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7856)
  AUTHORS   Konishi Y, Ichise H, Watabe T, Oki C, Tsukiji S, Hamazaki Y, 
            Murakawa Y, Takaori-Kondo A, Terai K, Matsuda M
  TITLE     Intravital imaging identifies the VEGF-TXA2 axis as a critical 
            promoter of PGE2 secretion from tumor cells and immune evasion.
  JOURNAL   Cancer Res. 2021 May 25. pii: 0008-5472.CAN-20-4245. doi: 
            10.1158/0008-5472.CAN-20-4245.
  PUBMED    34035084
REFERENCE   2  (bases 1 to 7856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2021 May 25. pii: 0008-5472.CAN-20-4245. doi: 
            10.1158/0008-5472.CAN-20-4245."
COMMENT     Sequence Label: pPBbsr2-Gnaq
FEATURES             Location/Qualifiers
     source          1..7856
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    87..636
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(231..248)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     458..477
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(1107..1124)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1113..1337
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(1384..1400)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1408..1424
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1432..1438,1439..1456,1457..1462))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1477..1498
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   1732..1794
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(1835..1855)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1835..1853)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1874..1890)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1898..1914
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1922..1928,1929..1946,1947..1952))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1967..1988
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2105..2122)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2276..2864)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2356..2375)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3035..3826,3827..3895))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3658..3677
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3896..4000)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4026..4481)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4163..4184)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4375..4394
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4608..4630
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4622..4639
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4623..4639
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4649..4668
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4649..4667
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   4697..4731
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        5034..5413
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          5691..6707
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     6631..6653
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     6715..6734
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
ORIGIN
        1 gtcgacgggc cgcggaaaca attgttaact aacttaagct agcaacggtt tccctctagc
       61 gggatcaatt ccgccccccc cccctaacgt tactggccga agccgcttgg aataaggccg
      121 gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc
      181 ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa
      241 aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag
      301 acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg
      361 cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg
      421 ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa
      481 caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg
      541 gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca
      601 cggggacgtg gttttccttt gaaaaacacg ataataccat ggtcatgaaa acatttaaca
      661 tttctcaaca agatctagaa ttagtagaag tagcgacaga gaagattaca atgctttatg
      721 aggataataa acatcatgtg ggagcggcaa ttcgtacgaa aacaggagaa atcatttcgg
      781 cagtacatat tgaagcgtat ataggacgag taactgtttg tgcagaagcc attgcgattg
      841 gtagtgcagt ttcgaatgga caaaaggatt ttgacacgat tgtagctgtt agacaccctt
      901 attctgacga agtagataga agtattcgag tggtaagtcc ttgtggtatg tgtagggagt
      961 tgatttcaga ctatgcacca gattgttttg tgttaataga aatgaatggc aagttagtca
     1021 aaactacgat tgaagaactc attccactca aatatacccg aaattaaaag tcgagatgca
     1081 tcgatgatct agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     1141 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     1201 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     1261 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     1321 tgcggtgggc tctatggctt ctgaggcgga aagaacctgc agcccaagct tggcgtaatc
     1381 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     1441 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     1501 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc ggatcttaaa
     1561 agttttgtta ctttatagaa gaaattttga gtttttgttt ttttttaata aataaataaa
     1621 cataaataaa ttgtttgttg aatttattat tagtatgtaa gtgtaaatat aataaaactt
     1681 aatatctatt caaattaata aataaacctc gatatacaga ccgataaaac acatgcgtca
     1741 attttacgca tgattatctt taacgtacgt cacaatatga ttatctttct agggttaatc
     1801 tagtatacgc gtttaattaa tccagctttt gttcccttta gtgagggtta attgcgcgct
     1861 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     1921 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     1981 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     2041 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     2101 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     2161 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     2221 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     2281 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     2341 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     2401 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     2461 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     2521 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     2581 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     2641 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     2701 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     2761 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     2821 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     2881 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     2941 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     3001 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     3061 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     3121 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     3181 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     3241 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     3301 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     3361 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     3421 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     3481 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     3541 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     3601 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     3661 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     3721 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     3781 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     3841 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     3901 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     3961 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     4021 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     4081 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     4141 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     4201 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     4261 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     4321 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     4381 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     4441 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
     4501 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     4561 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     4621 ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg aattggggcg
     4681 cgccattcta gattaaccct agaaagatag tctgcgtaaa attgacgcat gcattcttga
     4741 aatattgctc tctctttcta aatagcgcga atccgtcgct gtgcatttag gacatctcag
     4801 tcgccgcttg gagctcccgt gaggcgtgct tgtcaatgcg gtaagtgtca ctgattttga
     4861 actataacga ccgcgtgagt caaaatgacg catgattatc ttttacgtga cttttaagat
     4921 ttaactcata cgataattat attgttattt catgttctac ttacgtgata acttattata
     4981 tatatatttt cttgttatag atatcaacta gaatgctagc atgggcccat ctcgacattg
     5041 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
     5101 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     5161 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     5221 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     5281 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     5341 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     5401 cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc
     5461 ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc
     5521 gggggggggg ggggsgcgcg ccrggggggg gggggggggg sgrggggsgg ggsggggsgr
     5581 ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg
     5641 cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg ggagtcgctg
     5701 cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg ccccggctct
     5761 gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct ccgggctgta
     5821 attagcgctt ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa agccttgagg
     5881 ggctccggga gggccctttg tgcgggggga gcggctcggg gggtgcgtgc gtgtgtgtgt
     5941 gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg
     6001 gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag cgcggccggg ggcggtgccc
     6061 cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg tgcgtggggg
     6121 ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc ccccctgcac ccccctcccc
     6181 gagttgctga gcacggcccg gcttcgggtg cggggctccg tacggggcgt ggcgcggggc
     6241 tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg gggccgcctc
     6301 gggccgggga gggctcgggg gaggggcgcg gcggcccccg gagcgccggc ggctgtcgag
     6361 gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg cagggacttc
     6421 ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg
     6481 ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag ggccttcgtg
     6541 cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg cggggggacg
     6601 gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct
     6661 ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg
     6721 tgctggttat tgtgctgtct catcattttg gcaaagaatt gggatccgaa ttcaccatga
     6781 ctctggagtc catcatggcg tgctgcctga gcgaggaggc caaggaagcc cggaggatca
     6841 acgacgagat cgagcggcag ctgcgcaggg acaagcgcga cgcccgccgg gagctcaagc
     6901 tgctgctgct ggggacaggg gagagtggca agagcacctt catcaagcag atgaggatca
     6961 tccacgggtc gggctactct gacgaagaca agcgcggctt caccaagctg gtgtatcaga
     7021 acatcttcac ggccatgcag gccatgatca gagcgatgga cacgctcaag atcccataca
     7081 agtatgaaca caataaggct catgcacaat tggttcgaga ggttgatgtg gagaaggtgt
     7141 ctgcttttga gaatccatat gtagatgcaa taaagagctt gtggaatgat cctggaatcc
     7201 aggagtgcta cgacagacga cgggaatatc agttatctga ctctaccaaa tactatctga
     7261 atgacttgga ccgtgtagcc gacccttcct atctgcctac acaacaagac gtgcttagag
     7321 ttcgagtccc cactacaggg atcatcgaat acccctttga cttacaaagt gtcattttca
     7381 gaatggtcga tgtagggggc caaaggtcag agagaagaaa atggatacac tgctttgaaa
     7441 atgtcacctc catcatgttt ctagtagcgc ttagcgaata tgatcaagtt cttgtggagt
     7501 cagacaatga gaaccgcatg gaggagagca aagcactctt tagaacaatt atcacctacc
     7561 cctggttcca gaacagtagc gtcatcttat ttctgaataa gaaagatctt ctagaggaga
     7621 aaatcatgta ttcccaccta gtcgactact tcccagaata tgatggaccc cagagagatg
     7681 cccaggcagc tcgagaattc atcctgaaaa tgttcgtgga cctgaacccc gacagtgaca
     7741 aaatcatcta ctcccacttc acgtgcgcca cagataccga gaacatccgc ttcgtctttg
     7801 cagccgtcaa ggacaccatc ctgcagctga acctgaagga gtacaatctg gtctaa
//
