LOCUS       sequence-334395-        9377 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector to co-express a lacZ control spsgRNA with 
            NLS-mScarlet-I.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9377)
  AUTHORS   Wang S, Matsumoto K, Lish SR, Cartagena-Rivera AX, Yamada KM
  TITLE     Budding epithelial morphogenesis driven by cell-matrix versus 
            cell-cell adhesion.
  JOURNAL   Cell. 2021 Jul 8;184(14):3702-3716.e30. doi: 
            10.1016/j.cell.2021.05.015. Epub 2021 Jun 15.
  PUBMED    34133940
REFERENCE   2  (bases 1 to 9377)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.05.015"; journalName: "Cell"; date: "2021-07-8-
            8"; volume: "184"; issue: "14"; pages: "3702-3716.e30"
COMMENT     Sequence Label: 
            pW188-lenti-spsgRNA-lacZ-pEF1s-NLS-mScarlet-I-P2A-BlastR
FEATURES             Location/Qualifiers
     source          1..9377
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             8..241
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(267..284)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    273..497
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      543..971
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(630..649)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     840..861
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(980..1000)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        985..1314
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1165..1300
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1227..1246
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        1362..1409
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             1428..1802
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1932..2053
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1969..1988)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2023..2042
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2102..2118)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2102..2118)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2115..2137)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2126..2142
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2150..2156,2157..2174,2175..2180))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2195..2216
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2333..2350)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2504..3092)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2584..2603)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3263..4054,4055..4123))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3886..3905
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4124..4228)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4303..4322)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4494..4873
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4875..5073
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5028..5048
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             5091..5271
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5318..5443
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5936..6169
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6354..6398
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6696..6813
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6863..7111
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     6863..6883
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     7034..7053
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        7133..7208
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        7265..7476
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             7504..7551
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7552..8244
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="VSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGGA
                     VTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRHS
                     TGGMDELYK"
     primer_bind     complement(7682..7700)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7950..7969
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             8254..8310
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             8311..8709
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8725..9313
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8778..8798)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9196..9207)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact
       61 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
      121 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      181 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      241 agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
      301 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
      361 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
      421 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
      481 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
      541 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
      601 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
      661 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
      721 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
      781 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
      841 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
      901 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
      961 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     1021 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     1081 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     1141 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     1201 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
     1261 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     1321 ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg
     1381 catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagttga
     1441 ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg
     1501 accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg
     1561 acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct
     1621 gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga
     1681 acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg
     1741 agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact
     1801 gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     1861 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     1921 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     1981 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     2041 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
     2101 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     2161 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     2221 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     2281 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     2341 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     2401 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     2461 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     2521 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     2581 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     2641 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     2701 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     2761 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     2821 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     2881 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     2941 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     3001 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     3061 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     3121 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     3181 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     3241 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     3301 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     3361 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     3421 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     3481 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     3541 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     3601 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     3661 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     3721 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     3781 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     3841 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     3901 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     3961 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     4021 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     4081 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     4141 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     4201 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
     4261 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
     4321 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
     4381 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
     4441 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
     4501 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
     4561 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     4621 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     4681 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     4741 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     4801 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     4861 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     4921 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     4981 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     5041 taggcgtgta cggtgggagg tctatataag cagcgcgttt tgcctgtact gggtctctct
     5101 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     5161 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     5221 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     5281 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     5341 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     5401 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     5461 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     5521 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     5581 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     5641 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     5701 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     5761 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg
     5821 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     5881 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     5941 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     6001 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     6061 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     6121 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     6181 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     6241 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     6301 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     6361 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     6421 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     6481 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     6541 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     6601 gtggagagag agacagagac agatccattc gattagtgaa cggatcggca ctgcgtgcgc
     6661 caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
     6721 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     6781 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
     6841 ccagtttggt taattagcta gcgagggcct atttcccatg attccttcat atttgcatat
     6901 acgatacaag gctgttagag agataattgg aattaatttg actgtaaaca caaagatatt
     6961 agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag ttttaaaatt
     7021 atgttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg atttcttggc
     7081 tttatatatc ttgtggaaag gacgaaacac cgtgcgaata cgcccacgcg atgttttaga
     7141 gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga
     7201 gtcggtgctt ttttcgtctc aaaggtcttg aaaggagtgg gaattggctc cggtgcccgt
     7261 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     7321 tgatccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     7381 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     7441 gttctttttc gcaacgggtt tgccgccaga acacaggacc ggttctagag cgctgccacc
     7501 atgaagcgac ctgccgccac aaagaaggct ggacaggcta agaagaagaa agtgagcaag
     7561 ggcgaggcag tgatcaagga gttcatgcgg ttcaaggtgc acatggaggg ctccatgaac
     7621 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
     7681 gccaagctga aggtgaccaa gggtggcccc ctgcccttct cctgggacat cctgtcccct
     7741 cagttcatgt acggctccag ggccttcatc aagcaccccg ccgacatccc cgactactat
     7801 aagcagtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc
     7861 gccgtgaccg tgacccagga cacctccctg gaggacggca ccctgatcta caaggtgaag
     7921 ctccgcggca ccaacttccc tcctgacggc cccgtaatgc agaagaagac aatgggctgg
     7981 gaagcgtcca ccgagcggtt gtaccccgag gacggcgtgc tgaagggcga cattaagatg
     8041 gccctgcgcc tgaaggacgg cggccgctac ctggcggact tcaagaccac ctacaaggcc
     8101 aagaagcccg tgcagatgcc cggcgcctac aacgtcgacc gcaagttgga catcacctcc
     8161 cacaacgagg actacaccgt ggtggaacag tacgaacgct ccgagggccg ccactccacc
     8221 ggcggcatgg acgagctgta caagggatcc ggcgcaacaa acttctctct gctgaaacaa
     8281 gccggagatg tcgaagagaa tcctggaccg atggccaagc ctttgtctca agaagaatcc
     8341 accctcattg aaagagcaac ggctacaatc aacagcatcc ccatctctga agactacagc
     8401 gtcgccagcg cagctctctc tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat
     8461 tttactgggg gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc tgcggcagct
     8521 ggcaacctga cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt gagcccctgc
     8581 ggacggtgcc gacaggtgct tctcgatctg catcctggga tcaaagccat agtgaaggac
     8641 agtgatggac agccgacggc agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg
     8701 gagggctaaa cgcgttaagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg
     8761 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     8821 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     8881 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     8941 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     9001 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     9061 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     9121 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     9181 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     9241 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     9301 gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     9361 actttttaaa agaaaag
//
