LOCUS       sequence-334406-       14591 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac vector to express GA-inducible VPR-Sa_dCas9 with hygromycin
            resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14591)
  AUTHORS   Wang S, Matsumoto K, Lish SR, Cartagena-Rivera AX, Yamada KM
  TITLE     Budding epithelial morphogenesis driven by cell-matrix versus 
            cell-cell adhesion.
  JOURNAL   Cell. 2021 Jul 8;184(14):3702-3716.e30. doi: 
            10.1016/j.cell.2021.05.015. Epub 2021 Jun 15.
  PUBMED    34133940
REFERENCE   2  (bases 1 to 14591)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.05.015"; journalName: "Cell"; date: "2021-07-8-
            8"; volume: "184"; issue: "14"; pages: "3702-3716.e30"
COMMENT     Sequence Label: pW210-pPB-GA-inducible-CRISPRa-sa_dCas9-VPR-hygR
FEATURES             Location/Qualifiers
     source          1..14591
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          285..1301
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1225..1247
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1309..1328
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1356..1365
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2424..2573
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             2580..2600
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3030..3401
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     CDS             3420..3989
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     misc_feature    join(4007..4580,4581..4583,4584..4592)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4174..4191)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4401..4420
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4623..5642
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             4623..5642
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     misc_feature    5680..6268
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5733..5753)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6151..6162)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6289..6788
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6324..6347)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6721..6740
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6830..6847
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             7121..7819
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             7841..7861
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             11042..11089
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     repeat_region   11279..11341
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(11392..11412)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(11392..11410)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11431..11447)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11431..11447)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11444..11466)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11455..11471
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11479..11485,11486..11503,11504..11509))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11524..11545
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11662..11679)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11833..12421)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11913..11932)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12592..13383,13384..13452))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13215..13234
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13453..13557)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(13583..14038)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13720..13741)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     13932..13951
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     14165..14187
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     14179..14196
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     14180..14196
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14206..14225
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        14206..14224
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   14254..14288
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac
       61 ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg
      121 ggggggggsg cgcgccrggs ggggsggggs ggggsgrggg gsggggsggg gsgrggsggr
      181 grggkgsggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc
      241 ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgcgct
      301 gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg ctctgactga
      361 ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc tgtaattagc
      421 gcttggttta atgacggctt gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc
      481 gggagggccc tttgtgcggg gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg
      541 ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg
      601 ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt
      661 gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag
      721 cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct gcacccccct ccccgagttg
      781 ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg gcgtggcgcg gggctcgccg
      841 tgccgggcgg ggggtggcgg caggkggggg tgccgggcgg ggcggggccg cctcgggccg
      901 gggrgggctc gggggrgggg cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg
      961 cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt
     1021 cccaaatctg tgcggagccg aaatctggga ggcgccgccg caccccctct agcgggcgcg
     1081 gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc
     1141 cgcgccgccg tccccttctc cctctccagc ctcggggctg tccgcggggg gacggctgcc
     1201 ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag
     1261 cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg
     1321 ttattgtgct gtctcatcat tttggcaaag aattcgccac catggctgcg agcgatgaag
     1381 ttaatcttat tgagagcaga acagtggttc ctctcaatac atgggtttta atatccaact
     1441 tcaaagtagc ctacaatatc cttcgtcgcc ctgatggaac ctttaaccga cacttagctg
     1501 agtatctaga ccgtaaagtc actgcaaacg ccaatccggt tgatggggtt ttctcgttcg
     1561 atgtcttgat tgatcgcagg atcaatcttc taagcagagt ctatagacca gcttatgcag
     1621 atcaagagca acctcctagt attttagatc tcgagaagcc tgttgatggc gacattgtcc
     1681 ctgttatatt gttcttccat ggaggtagct ttgctcattc ttctgcaaac agtgccatct
     1741 acgatactct ttgtcgcagg cttgttggtt tgtgcaagtg tgttgttgtc tctgtgaatt
     1801 atcggcgtgc accagagaat ccataccctt gtgcttatga tgatggttgg attgctctta
     1861 attgggttaa ctcgagatct tggcttaaat ccaagaaaga ctcaaaggtc catattttct
     1921 tggctggtga tagctctgga ggtaacatcg cgcataatgt ggctttaaga gcgggtgaat
     1981 cgggaatcga tgttttgggg aacattctgc tgaatcctat gtttggtggg aatgagagaa
     2041 cggagtctga gaaaagtttg gatgggaaat actttgtgac ggttagagac cgcgattggt
     2101 actggaaagc gtttttaccc gagggagaag atagagagca tccagcgtgt aatccgttta
     2161 gcccgagagg gaaaagctta gaaggagtga gtttccccaa gagtcttgtg gttgtcgcgg
     2221 gtttggattt gattagagat tggcagttgg catacgcgga agggctcaag aaagcgggtc
     2281 aagaggttaa gcttatgcat ttagagaaag caactgttgg gttttacctc ttgcctaata
     2341 acaatcattt ccataatgtt atggatgaga tttcggcgtt tgtaaacgcg gaatgtgaat
     2401 tctcaagcgc tgcaggtact agtgatgctt tagacgattt tgacttagat atgcttggtt
     2461 cagacgcgtt agacgacttc gacctagaca tgttaggctc agatgcattg gacgacttcg
     2521 atttagatat gttgggctcc gatgccctag atgactttga tctagatatg ctaggtagtc
     2581 ccaaaaagaa gaggaaagtg ggatcccagt atctgcccga cacagatgat agacaccgaa
     2641 tcgaagagaa acgcaagcga acgtatgaaa ccttcaaatc gatcatgaag aaatcgccct
     2701 tctcgggtcc gaccgatccc aggcccccac cgagaaggat tgcggtcccg tcccgctcgt
     2761 cggccagcgt gccgaagcct gcgccgcagc cctacccctt cacgtcgagc ctgagcacaa
     2821 tcaattatga cgagttcccg acgatggtgt tcccctcggg acaaatctca caagcctcgg
     2881 cgctcgcacc agcgcctccc caagtccttc cgcaagcgcc tgccccagcg cctgcaccgg
     2941 caatggtgtc cgccctcgca caggcccctg cgcccgtccc cgtgctcgcg cctggaccgc
     3001 cccaggcggt cgctccaccg gctccgaagc cgacgcaggc cggagaggga acactctccg
     3061 aagcacttct tcaactccag tttgatgacg aggatcttgg agcactcctt ggaaactcga
     3121 cagaccctgc ggtgtttacc gacctcgcgt cagtagataa ctccgaattt cagcagcttt
     3181 tgaaccaggg tatcccggtc gcgccacata caacggagcc catgttgatg gaataccccg
     3241 aagcaatcac gagacttgtg acgggagcgc agcggcctcc cgatcccgca cccgcacctt
     3301 tgggggcacc tggcctccct aacggacttt tgagcggcga cgaggatttc tcctccatcg
     3361 ccgatatgga tttctcagcc ttgctgtcac agatttccag cggctctggc agcggcagcc
     3421 gggattccag ggaagggatg tttttgccga agcctgaggc cggctccgct attagtgacg
     3481 tgtttgaggg ccgcgaggtg tgccagccaa aacgaatccg gccatttcat cctccaggaa
     3541 gtccatgggc caaccgccca ctccccgcca gcctcgcacc aacaccaacc ggtccagtac
     3601 atgagccagt cgggtcactg accccggcac cagtccctca gccactggat ccagcgcccg
     3661 cagtgactcc cgaggccagt cacctgttgg aggatcccga tgaagagacg agccaggctg
     3721 tcaaagccct tcgggagatg gccgatactg tgattcccca gaaggaagag gctgcaatct
     3781 gtggccaaat ggacctttcc catccgcccc caaggggcca tctggatgag ctgacaacca
     3841 cacttgagtc catgaccgag gatctgaacc tggactcacc cctgaccccg gaattgaacg
     3901 agattctgga taccttcctg aacgacgagt gcctcttgca tgccatgcat atcagcacag
     3961 gactgtccat cttcgacaca tctctgtttt gaatttaaat aattcgcccc tctccctccc
     4021 ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata
     4081 tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg
     4141 tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt
     4201 tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag
     4261 cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc
     4321 cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga
     4381 tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg
     4441 cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacgt
     4501 gtgtttagtc gaggttaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct
     4561 ttgaaaaaca cgatgataat atggccacaa ccggatccgg agctaccatg gatagatccg
     4621 gaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg
     4681 tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag
     4741 gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt
     4801 atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg
     4861 aattcagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag
     4921 acctgcctga aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga
     4981 tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg
     5041 gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact
     5101 ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga
     5161 tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca
     5221 acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt
     5281 tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta
     5341 tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc
     5401 tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca
     5461 atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg
     5521 ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg
     5581 tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat
     5641 aggtttaaac catgcaagct tgatatcaag cttatcgata atcaacctct ggattacaaa
     5701 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     5761 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     5821 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     5881 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     5941 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     6001 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     6061 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     6121 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     6181 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     6241 cggatctccc tttgggccgc ctccccgcat cctgcaggta attctaccgg gtaggggagg
     6301 cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct
     6361 acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc
     6421 cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc
     6481 ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactaggc
     6541 tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc
     6601 aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg
     6661 gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg
     6721 cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg
     6781 gcctttcgac ctgcagccca agcttgtcga cgccaccatg aagagagatc atcatcatca
     6841 tcatcatcaa gataagaaga ctatgatgat gaatgaagaa gacgacggta acggcatgga
     6901 tgagcttcta gctgttcttg gttacaaggt taggtcatcc gaaatggctg atgttgctca
     6961 gaaactcgag cagcttgaag ttatgatgtc taatgttcaa gaagacgatc tttctcaact
     7021 cgctactgag actgttcact ataatccggc ggagctttac acgtggcttg attctatgct
     7081 caccgacctt aatcatatgt caagcgctgc aggtgctacc atgagcgagc tgattaagga
     7141 gaacatgcac atgaagctgt acatggaggg caccgtggac aaccatcact tcaagtgcac
     7201 atccgagggc gaaggcaagc cctacgaggg cacccagacc atgagaatca aggtggtcga
     7261 gggcggccct ctccccttcg ccttcgacat cctggctact agcttcctct acggcagcaa
     7321 gaccttcatc aaccacaccc agggcatccc cgacttcttc aagcagtcct tccctgaggg
     7381 cttcacatgg gagagagtca ccacatacga agacgggggc gtgctgaccg ctacccagga
     7441 caccagcctc caggacggct gcctcatcta caacgtcaag atcagagggg tgaacttcac
     7501 atccaacggc cctgtgatgc agaagaaaac actcggctgg gaggccttca ccgagacgct
     7561 gtaccccgct gacggcggcc tggaaggcag aaacgacatg gccctgaagc tcgtgggcgg
     7621 gagccatctg atcgcaaaca tcaagaccac atatagatcc aagaaacccg ctaagaacct
     7681 caagatgcct ggcgtctact atgtggacta cagactggaa agaatcaagg aggccaacaa
     7741 cgagacctac gtcgagcagc acgaggtggc agtggccaga tactgcgacc tccctagcaa
     7801 actggggcac aagcttaatg gtggaggctc tggaatggcc ccaaagaaga agcggaaggt
     7861 cggtatccac ggagtcccag cagccaagcg gaactacatc ctgggcctgg ccatcggcat
     7921 caccagcgtg ggctacggca tcatcgacta cgagacacgg gacgtgatcg atgccggcgt
     7981 gcggctgttc aaagaggcca acgtggaaaa caacgagggc aggcggagca agagaggcgc
     8041 cagaaggctg aagcggcgga ggcggcatag aatccagaga gtgaagaagc tgctgttcga
     8101 ctacaacctg ctgaccgacc acagcgagct gagcggcatc aacccctacg aggccagagt
     8161 gaagggcctg agccagaagc tgagcgagga agagttctct gccgccctgc tgcacctggc
     8221 caagagaaga ggcgtgcaca acgtgaacga ggtggaagag gacaccggca acgagctgtc
     8281 caccaaagag cagatcagcc ggaacagcaa ggccctggaa gagaaatacg tggccgaact
     8341 gcagctggaa cggctgaaga aagacggcga agtgcggggc agcatcaaca gattcaagac
     8401 cagcgactac gtgaaagaag ccaaacagct gctgaaggtg cagaaggcct accaccagct
     8461 ggaccagagc ttcatcgaca cctacatcga cctgctggaa acccggcgga cctactatga
     8521 gggacctggc gagggcagcc ccttcggctg gaaggacatc aaagaatggt acgagatgct
     8581 gatgggccac tgcacctact tccccgagga actgcggagc gtgaagtacg cctacaacgc
     8641 cgacctgtac aacgccctga acgacctgaa caatctcgtg atcaccaggg acgagaacga
     8701 gaagctggaa tattacgaga agttccagat catcgagaac gtgttcaagc agaagaagaa
     8761 gcccaccctg aagcagatcg ccaaagaaat cctcgtgaac gaagaggata ttaagggcta
     8821 cagagtgacc agcaccggca agcccgagtt caccaacctg aaggtgtacc acgacatcaa
     8881 ggacattacc gcccggaaag agattattga gaacgccgag ctgctggatc agattgccaa
     8941 gatcctgacc atctaccaga gcagcgagga catccaggaa gaactgacca atctgaactc
     9001 cgagctgacc caggaagaga tcgagcagat ctctaatctg aagggctata ccggcaccca
     9061 caacctgagc ctgaaggcca tcaacctgat cctggacgag ctgtggcaca ccaacgacaa
     9121 ccagatcgct atcttcaacc ggctgaagct ggtgcccaag aaggtggacc tgtcccagca
     9181 gaaagagatc cccaccaccc tggtggacga cttcatcctg agccccgtcg tgaagagaag
     9241 cttcatccag agcatcaaag tgatcaacgc catcatcaag aagtacggcc tgcccaacga
     9301 catcattatc gagctggccc gcgagaagaa ctccaaggac gcccagaaaa tgatcaacga
     9361 gatgcagaag cggaaccggc agaccaacga gcggatcgag gaaatcatcc ggaccaccgg
     9421 caaagagaac gccaagtacc tgatcgagaa gatcaagctg cacgacatgc aggaaggcaa
     9481 gtgcctgtac agcctggaag ccatccctct ggaagatctg ctgaacaacc ccttcaacta
     9541 tgaggtggac cacatcatcc ccagaagcgt gtccttcgac aacagcttca acaacaaggt
     9601 gctcgtgaag caggaagaag ccagcaagaa gggcaaccgg accccattcc agtacctgag
     9661 cagcagcgac agcaagatca gctacgaaac cttcaagaag cacatcctga atctggccaa
     9721 gggcaagggc agaatcagca agaccaagaa agagtatctg ctggaagaac gggacatcaa
     9781 caggttctcc gtgcagaaag acttcatcaa ccggaacctg gtggatacca gatacgccac
     9841 cagaggcctg atgaacctgc tgcggagcta cttcagagtg aacaacctgg acgtgaaagt
     9901 gaagtccatc aatggcggct tcaccagctt tctgcggcgg aagtggaagt ttaagaaaga
     9961 gcggaacaag gggtacaagc accacgccga ggacgccctg atcattgcca acgccgattt
    10021 catcttcaaa gagtggaaga aactggacaa ggccaaaaaa gtgatggaaa accagatgtt
    10081 cgaggaaaag caggccgaga gcatgcccga gatcgaaacc gagcaggagt acaaagagat
    10141 cttcatcacc ccccaccaga tcaagcacat taaggacttc aaggactaca agtacagcca
    10201 ccgggtggac aagaagccta atagagagct gattaacgac accctgtact ccacccggaa
    10261 ggacgacaag ggcaacaccc tgatcgtgaa caatctgaac ggcctgtacg acaaggacaa
    10321 tgacaagctg aaaaagctga tcaacaagag ccccgaaaag ctgctgatgt accaccacga
    10381 cccccagacc taccagaaac tgaagctgat tatggaacag tacggcgacg agaagaatcc
    10441 cctgtacaag tactacgagg aaaccgggaa ctacctgacc aagtactcca aaaaggacaa
    10501 cggccccgtg atcaagaaga ttaagtatta cggcaacaaa ctgaacgccc atctggacat
    10561 caccgacgac taccccaaca gcagaaacaa ggtcgtgaag ctgtccctga agccctacag
    10621 attcgacgtg tacctggaca atggcgtgta caagttcgtg accgtgaaga atctggatgt
    10681 gatcaaaaaa gaaaactact acgaagtgaa tagcaagtgc tatgaggaag ctaagaagct
    10741 gaagaagatc agcaaccagg ccgagtttat cgcctccttc tacaacaacg atctgatcaa
    10801 gatcaacggc gagctgtata gagtgatcgg cgtgaacaac gacctgctga accggatcga
    10861 agtgaacatg atcgacatca cctaccgcga gtacctggaa aacatgaacg acaagaggcc
    10921 ccccaggatc attaagacaa tcgcctccaa gacccagagc attaagaagt acagcacaga
    10981 cattctgggc aacctgtatg aagtgaaatc taagaagcac cctcagatca tcaaaaaggg
    11041 caaaaggccg gcggccacga aaaaggccgg ccaggcaaaa aagaaaaagt aactcgagct
    11101 agttaaaagt tttgttactt tatagaagaa attttgagtt tttgtttttt tttaataaat
    11161 aaataaacat aaataaattg tttgttgaat ttattattag tatgtaagtg taaatataat
    11221 aaaacttaat atctattcaa attaataaat aaacctcgat atacagaccg ataaaacaca
    11281 tgcgtcaatt ttacgcatga ttatctttaa cgtacgtcac aatatgatta tctttctagg
    11341 gttaatctag tatacgcgta tgcggccgct taattaatcc agcttttgtt ccctttagtg
    11401 agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
    11461 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
    11521 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
    11581 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
    11641 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
    11701 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
    11761 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
    11821 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
    11881 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
    11941 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
    12001 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
    12061 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
    12121 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
    12181 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
    12241 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
    12301 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
    12361 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
    12421 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
    12481 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
    12541 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
    12601 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
    12661 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
    12721 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
    12781 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
    12841 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
    12901 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
    12961 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
    13021 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
    13081 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
    13141 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
    13201 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    13261 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    13321 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    13381 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    13441 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    13501 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    13561 atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta
    13621 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
    13681 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
    13741 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
    13801 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
    13861 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
    13921 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
    13981 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc
    14041 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
    14101 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
    14161 ttttcccagt cacgacgttg taaaacgacg gccagtgagc gcgcgtaata cgactcacta
    14221 tagggcgaat tggggcgcgc cattctagat taaccctaga aagatagtct gcgtaaaatt
    14281 gacgcatgca ttcttgaaat attgctctct ctttctaaat agcgcgaatc cgtcgctgtg
    14341 catttaggac atctcagtcg ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta
    14401 agtgtcactg attttgaact ataacgaccg cgtgagtcaa aatgacgcat gattatcttt
    14461 tacgtgactt ttaagattta actcatacga taattatatt gttatttcat gttctactta
    14521 cgtgataact tattatatat atattttctt gttatagata tcaactagaa tgctagcatg
    14581 ggcccagatc t
//
