LOCUS       sequence-33441-e         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Radoshevich L, Murrow L, Chen N, Fernandez E, Roy S, Fung C, Debnath
            J
  TITLE     ATG12 conjugation to ATG3 regulates mitochondrial homeostasis and 
            cell death.
  JOURNAL   Cell. 2010 Aug 20. 142(4):590-600.
  PUBMED    20723759
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2010 
            Aug 20. 142(4):590-600."
COMMENT     Sequence Label: pBABEpuro WT ATG3
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(7..24)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(34..75)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 tccgtcatgg tgatggtgat gatgaccggt acgcgtagaa tcgagaccga ggagagggtt
       61 agggataggc ttaccttcga accgcgggcc ctctagactc gagcggccgc aaatcattgt
      121 gaagtgtctt gtgtagtcat attctattgt tggaatgaca gcttgaacaa atttcaaaaa
      181 aattaaaaga tacatatgaa caccaagctc tcccccgcct tctgcaactg tctcaataat
      241 tttcttcatc acttcagcat gcctgcatgg gtgaactgaa cacataggag gtggtgggag
      301 atgaggatgg ttttcaatgg tcactgtttt cttcacatga tcttgactga tgtcttcata
      361 catgtgctca actgttaaag gctgccgttg ctcatcatag ccaaacaacc atagccgtgg
      421 tgtctggtaa tatttgtcgt aagtgatgta cagatcgtat gttctcgttt gtaaaatagc
      481 atcttcacct ccagcgtcag ccttagcttt gcaggcttcc actattttcc ttgtgtctag
      541 ggtagcctca tctgtttcca acaatccact ctcttcatat tcttccatgt ctgcagcttc
      601 cccttcatct tcctcgtctt cttcatcaca cagtgctgag caatcttgga gttttatact
      661 gtccttgctt tccagtgtaa tctccttaac tgcttcagta attcctgtaa tacctgtgtt
      721 gtgatatgta tctacccatc ccccatcacc atcatcttct tcaatgatag cttccaattc
      781 atccgaatac tccatctgtt tacaccgctt gtagcatgga acatttttgg ttaccaaaaa
      841 ttgtttgtct gtcggaagat atgccttcac tttcaattct tcccctgtag cccattgcca
      901 tgttggacag tggtggacta agtgatctcc agctgccaca aactcttctg
//
