LOCUS       sequence-334411-       11447 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral Expression of EF1a.SLC6A1.S295L.RFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11447)
  TITLE     Rarebase PBC plasmids
REFERENCE   2  (bases 1 to 11447)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MS2592.EF1a.SLC6A1.S295L.RFP.P2A.PURO.JL.p163
FEATURES             Location/Qualifiers
     source          1..11447
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2413..2624
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2662..2671
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2668..3342
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             2668..3342
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(2798..2816)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3066..3085
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3260..3283
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             5239..5295
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5296..5892
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     5789..5809
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5923..6511
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5976..5996)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6394..6405)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6514..6530)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6515..6531)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7036..7216
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7242..7259)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7248..7472
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7518..7946
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7605..7624)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7815..7836
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7955..7975)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8120..8254
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8181..8200
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8316..8363
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8382..8756
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8886..9007
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8923..8942)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8977..8996
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9056..9072)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9056..9072)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9069..9091)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9080..9096
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9104..9110,9111..9128,9129..9134))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9149..9170
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9287..9304)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9458..10046)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9538..9557)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10217..11008,11009..11077))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10840..10859
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11078..11182)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11257..11276)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttaa ttagctagct aggtcttgaa aggagtggga attggctccg
     2401 gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg
     2461 tcggcaattg atccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg
     2521 tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg
     2581 ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggaccgg ttctagacgt
     2641 acgctagagg atcgaaccct tgccaccatg gcctcctccg aggacgtcat caaggagttc
     2701 atgcgcttca aggtgcgcat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
     2761 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc
     2821 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tccagtacgg ctccaaggcc
     2881 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
     2941 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
     3001 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     3061 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga gcggatgtac
     3121 cccgaggacg gcgccctgaa gggcgagatc aagatgaggc tgaagctgaa ggacggcggc
     3181 cactacgacg ccgaggtcaa gaccacctac atggccaaga agcccgtgca gctgcccggc
     3241 gcctacaaga ccgacatcaa gctggacatc acctcccaca acgaggacta caccatcgtg
     3301 gaacagtacg agcgcgccga gggccgccac tccaccggcg ccggtggagg aggttctgga
     3361 ggcggtggaa gtggtggcgg aggtagccca cctcctccaa gacctccgcc accttcacca
     3421 ccgcctccat cgatggcgac caacggcagc aaggtggccg acgggcagat ctccaccgag
     3481 gtcagcgagg cccctgtggc caatgacaag cccaaaacct tggtggtcaa ggtgcagaag
     3541 aaggcggcag acctccccga ccgggacacg tggaagggcc gcttcgactt cctcatgtcc
     3601 tgtgtgggct atgccatcgg cctgggcaac gtctggaggt tcccctatct ctgcgggaaa
     3661 aatggtgggg gagccttcct gatcccctat ttcctgacac tcatctttgc gggggtccca
     3721 ctcttcctgc tggagtgctc cctgggccag tacacctcca tcggggggct aggggtatgg
     3781 aagctggctc ctatgttcaa gggcgtgggc cttgcggctg ctgtgctatc attctggctg
     3841 aacatctact acatcgtcat catctcctgg gccatttact acctgtacaa ctccttcacc
     3901 acgacactgc cgtggaaaca gtgcgacaac ccctggaaca cagaccgctg cttctccaac
     3961 tacagcatgg tcaacactac caacatgacc agcgctgtgg tggagttctg ggagcgcaac
     4021 atgcatcaga tgacggacgg gctggataag ccaggtcaga tccgctggcc actggccatc
     4081 acgctggcca tcgcctggat ccttgtgtat ttctgtatct ggaagggtgt tggctggact
     4141 ggaaaggtgg tctacttttc agccacatac ccctacatca tgctgatcat cctgttcttc
     4201 cgtggagtga cgctgcccgg ggccaaggag ggcatcctct tctacatcac acccaacttc
     4261 cgcaagctgt ctgactccga ggtgtggctg gatgcggcaa cccagatctt cttcttatac
     4321 gggctgggcc tggggtccct gatcgctctc gggagctaca actctttcca caacaatgtc
     4381 tacagggact ccatcatcgt ctgctgcatc aattcgtgca ccagcatgtt cgcaggattc
     4441 gtcatcttct ccatcgtggg cttcatggcc catgtcacca agaggtccat tgctgatgtg
     4501 gcggcctcag gccccgggct ggcgttcctg gcatacccag aggcggtgac ccagctgcct
     4561 atctccccac tctgggccat cctcttcttc tccatgctgt tgatgctggg cattgacagc
     4621 cagttctgca ctgtggaggg cttcatcaca gccctggtgg atgagtaccc caggctcctc
     4681 cgcaaccgca gagagctctt cattgctgct gtctgcatca tctcctacct gatcggtctc
     4741 tctaacatca ctcagggggg tatttatgtc ttcaaactct ttgactacta ctctgccagt
     4801 ggcatgagcc tgctgttcct cgtgttcttt gaatgtgtct ctatttcctg gttttacggt
     4861 gtcaaccgat tctatgacaa tatccaagag atggttggat ccaggccctg catctggtgg
     4921 aaactctgct ggtctttctt cacaccaatc attgtggcgg gcgtgttcat tttcagtgct
     4981 gtgcagatga cgccactcac catgggaaac tatgttttcc ccaagtgggg ccagggtgtg
     5041 ggctggctga tggctctgtc ttccatggtc ctcatccccg ggtacatggc ctacatgttc
     5101 ctcaccttaa agggctccct gaagcagcgc atccaagtca tggtccagcc cagcgaagac
     5161 atcgttcgcc cagagaatgg tcctgagcag ccccaggcgg gcagctccac cagcaaggag
     5221 gcctacatcg gatctggtgc cacgaatttc tcgctactca agcaggccgg tgatgtcgag
     5281 gaaaaccctg gtcctatgga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     5341 cccagggctg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     5401 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     5461 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     5521 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     5581 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     5641 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     5701 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     5761 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     5821 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     5881 cccggtgcct gagaattcga attcgatatc aagcttatcg gtaatcaacc tctggattac
     5941 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     6001 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     6061 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     6121 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     6181 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     6241 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     6301 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     6361 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     6421 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     6481 agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg agacctagaa
     6541 aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta
     6601 gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca
     6661 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     6721 gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa
     6781 ggctacttcc ctgattggca gaactacaca ccagggccag ggatcagata tccactgacc
     6841 tttggatggt gctacaagct agtaccagtt gagcaagaga aggtagaaga agccaatgaa
     6901 ggagagaaca cccgcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga
     6961 gaagtattag agtggaggtt tgacagccgc ctagcatttc atcacatggc ccgagagctg
     7021 catccggact gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     7081 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     7141 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     7201 tggaaaatct ctagcagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag
     7261 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     7321 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     7381 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     7441 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
     7501 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     7561 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     7621 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     7681 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     7741 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     7801 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     7861 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     7921 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga
     7981 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     8041 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     8101 aattagtcag caaccatagt cccgccccta actccgccca gttccgccca ttctccgccc
     8161 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
     8221 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
     8281 gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt
     8341 atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag ttgaccagtg
     8401 ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc
     8461 tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga
     8521 ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt
     8581 gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc
     8641 gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg
     8701 ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg
     8761 tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
     8821 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
     8881 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     8941 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     9001 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
     9061 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     9121 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     9181 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     9241 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     9301 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     9361 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     9421 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     9481 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     9541 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     9601 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     9661 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     9721 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     9781 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     9841 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     9901 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     9961 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
    10021 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
    10081 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
    10141 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
    10201 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
    10261 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
    10321 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
    10381 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
    10441 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
    10501 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
    10561 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
    10621 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
    10681 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
    10741 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
    10801 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
    10861 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
    10921 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    10981 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    11041 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    11101 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    11161 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
    11221 cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata
    11281 gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa
    11341 aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt
    11401 taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgtt
//
