LOCUS       sequence-334412-       10682 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral Expression of EF1a.SLC6A1.A288V.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10682)
  TITLE     Rarebase PBC plasmids
REFERENCE   2  (bases 1 to 10682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MS2592.EF1a.SLC6A1.A288V.P2A.PURO.JL.p166
FEATURES             Location/Qualifiers
     source          1..10682
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2413..2624
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2662..2671
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4474..4530
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4531..5127
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     5024..5044
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5158..5746
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5211..5231)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5629..5640)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5749..5765)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5750..5766)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6271..6451
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6477..6494)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6483..6707
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6753..7181
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6840..6859)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7050..7071
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7190..7210)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7355..7489
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7416..7435
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7551..7598
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7617..7991
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8121..8242
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8158..8177)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8212..8231
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8291..8307)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8291..8307)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8304..8326)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8315..8331
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8339..8345,8346..8363,8364..8369))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8384..8405
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8522..8539)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8693..9281)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8773..8792)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9452..10243,10244..10312))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10075..10094
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10313..10417)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10492..10511)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttaa ttagctagct aggtcttgaa aggagtggga attggctccg
     2401 gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg
     2461 tcggcaattg atccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg
     2521 tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg
     2581 ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggaccgg ttctagacgt
     2641 acgctagagg atcgaaccct tgccaccatg gcgaccaacg gcagcaaggt ggccgacggg
     2701 cagatctcca ccgaggtcag cgaggcccct gtggccaatg acaagcccaa aaccttggtg
     2761 gtcaaggtgc agaagaaggc ggcagacctc cccgaccggg acacgtggaa gggccgcttc
     2821 gacttcctca tgtcctgtgt gggctatgcc atcggcctgg gcaacgtctg gaggttcccc
     2881 tatctctgcg ggaaaaatgg tgggggagcc ttcctgatcc cctatttcct gacactcatc
     2941 tttgcggggg tcccactctt cctgctggag tgctccctgg gccagtacac ctccatcggg
     3001 gggctagggg tatggaagct ggctcctatg ttcaagggcg tgggccttgc ggctgctgtg
     3061 ctatcattct ggctgaacat ctactacatc gtcatcatct cctgggccat ttactacctg
     3121 tacaactcct tcaccacgac actgccgtgg aaacagtgcg acaacccctg gaacacagac
     3181 cgctgcttct ccaactacag catggtcaac actaccaaca tgaccagcgc tgtggtggag
     3241 ttctgggagc gcaacatgca tcagatgacg gacgggctgg ataagccagg tcagatccgc
     3301 tggccactgg ccatcacgct ggccatcgcc tggatccttg tgtatttctg tatctggaag
     3361 ggtgttggct ggactggaaa ggtggtctac ttttcagcca cataccccta catcatgctg
     3421 atcatcctgt tcttccgtgg agtgacgctg cccggggcca aggagggcat cctcttctac
     3481 atcacaccca acttccgcaa gctgtctgac tccgaggtgt ggctggatgt ggcaacccag
     3541 atcttcttct catacgggct gggcctgggg tccctgatcg ctctcgggag ctacaactct
     3601 ttccacaaca atgtctacag ggactccatc atcgtctgct gcatcaattc gtgcaccagc
     3661 atgttcgcag gattcgtcat cttctccatc gtgggcttca tggcccatgt caccaagagg
     3721 tccattgctg atgtggcggc ctcaggcccc gggctggcgt tcctggcata cccagaggcg
     3781 gtgacccagc tgcctatctc cccactctgg gccatcctct tcttctccat gctgttgatg
     3841 ctgggcattg acagccagtt ctgcactgtg gagggcttca tcacagccct ggtggatgag
     3901 taccccaggc tcctccgcaa ccgcagagag ctcttcattg ctgctgtctg catcatctcc
     3961 tacctgatcg gtctctctaa catcactcag gggggtattt atgtcttcaa actctttgac
     4021 tactactctg ccagtggcat gagcctgctg ttcctcgtgt tctttgaatg tgtctctatt
     4081 tcctggtttt acggtgtcaa ccgattctat gacaatatcc aagagatggt tggatccagg
     4141 ccctgcatct ggtggaaact ctgctggtct ttcttcacac caatcattgt ggcgggcgtg
     4201 ttcattttca gtgctgtgca gatgacgcca ctcaccatgg gaaactatgt tttccccaag
     4261 tggggccagg gtgtgggctg gctgatggct ctgtcttcca tggtcctcat ccccgggtac
     4321 atggcctaca tgttcctcac cttaaagggc tccctgaagc agcgcatcca agtcatggtc
     4381 cagcccagcg aagacatcgt tcgcccagag aatggtcctg agcagcccca ggcgggcagc
     4441 tccaccagca aggaggccta catcggatct ggtgccacga atttctcgct actcaagcag
     4501 gccggtgatg tcgaggaaaa ccctggtcct atggagtaca agcccacggt gcgcctcgcc
     4561 acccgcgacg acgtccccag ggctgtacgc accctcgccg ccgcgttcgc cgactacccc
     4621 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     4681 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     4741 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     4801 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     4861 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     4921 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     4981 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     5041 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     5101 tgcatgaccc gcaagcccgg tgcctgagaa ttcgaattcg atatcaagct tatcggtaat
     5161 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     5221 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     5281 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     5341 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     5401 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     5461 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     5521 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     5581 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     5641 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     5701 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga
     5761 cctcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct
     5821 gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag
     5881 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     5941 aaggggggac tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg
     6001 atctaccaca cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc
     6061 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagca agagaaggta
     6121 gaagaagcca atgaaggaga gaacacccgc ttgttacacc ctgtgagcct gcatgggatg
     6181 gatgacccgg agagagaagt attagagtgg aggtttgaca gccgcctagc atttcatcac
     6241 atggcccgag agctgcatcc ggactgtact gggtctctct ggttagacca gatctgagcc
     6301 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     6361 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     6421 cccttttagt cagtgtggaa aatctctagc agggcccgtt taaacccgct gatcagcctc
     6481 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     6541 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     6601 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     6661 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     6721 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     6781 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     6841 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     6901 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     6961 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     7021 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     7081 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     7141 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
     7201 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     7261 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     7321 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccagttcc
     7381 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     7441 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     7501 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt
     7561 aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg
     7621 ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt
     7681 tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg
     7741 tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca
     7801 ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg
     7861 tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt
     7921 gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg
     7981 agcaggactg acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg
     8041 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     8101 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
     8161 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     8221 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
     8281 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     8341 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     8401 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     8461 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     8521 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     8581 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     8641 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     8701 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     8761 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     8821 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     8881 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     8941 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     9001 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     9061 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     9121 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     9181 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     9241 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     9301 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     9361 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     9421 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     9481 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     9541 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     9601 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     9661 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     9721 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     9781 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     9841 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     9901 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     9961 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
    10021 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
    10081 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
    10141 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
    10201 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
    10261 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
    10321 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
    10381 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
    10441 ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg
    10501 ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga
    10561 gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa
    10621 gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg
    10681 tt
//
