LOCUS       sequence-334416-        2352 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick - pG10-90.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Gonzalez-Grandio E, Demirer GS, Ma W, Brady S, Landry MP
  TITLE     A Ratiometric Dual Color Luciferase Reporter for Fast 
            Characterization of Transcriptional Regulatory Elements in Plants.
  JOURNAL   ACS Synth Biol. 2021 Sep 14. doi: 10.1021/acssynbio.1c00248.
  PUBMED    34520169
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2021 Sep 14. doi: 10.1021/acssynbio.1c00248."
COMMENT     Sequence Label: pUPD2+pG10-90
FEATURES             Location/Qualifiers
     source          1..2352
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(346..440)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(461..1120)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1035..1054
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1282..1300
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1304..1347)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1350..1371
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     primer_bind     1565..1585
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        1574..1620
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     terminator      1675..1732
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1756..1773)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(1927..2352,1..163))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2007..2026)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
       61 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      121 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
      181 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
      241 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
      301 ctaaagtata tatgagtaaa cttggtctga cagctcgagg cttggattct caccaataaa
      361 aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac
      421 tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg ccctgccact
      481 cgtcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg
      541 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc
      601 ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg
      661 tgaaactcac ccagggattg gctgatacga aaaacatatt ctcaataaac cctttaggga
      721 aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc
      781 ggaaatcgtc gtggtattca ctccagagtg atgaaaacgt ttcagtttgc tcatggaaaa
      841 cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac
      901 gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact
      961 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt
     1021 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg
     1081 atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg
     1141 aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt
     1201 tggaacctct tacgtgcccg atcaactcga gtgccacctg acgtctaaga aaccattatt
     1261 atcatgacat taacctataa aaataggcgt atcacgaggc agaatttcag ataaaaaaaa
     1321 tccttagctt tcgctaagga tgatttctgg aattcgcggc cgcttctaga gcgatgagtg
     1381 gtctcgggag atagtttaaa ctgaaggcgg gaaacgacaa tctgatccaa gctcaagcta
     1441 agcttgcatg cctgcaggat atcgtggatc caagcttgcc acgtgccgcc acgtgccgcc
     1501 acgtgccgcc acgtgcctct agaggatcca tctccactga cgtaagggat gacgcacaat
     1561 cccactatcc ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagga
     1621 cacgctggga tccaatgtga gaccactcat cgcgactagt agcggccgct gcagtccggc
     1681 aaaaaagggc aaggtgtcac caccctgccc tttttcttta aaaccgaaaa gattacttcg
     1741 cgttatgcag gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     1801 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     1861 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     1921 ggcgtttttc cacaggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     1981 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     2041 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     2101 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     2161 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     2221 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     2281 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     2341 gtggcctaac ta
//
