LOCUS       sequence-334419-        2595 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick - NtEUt.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Gonzalez-Grandio E, Demirer GS, Ma W, Brady S, Landry MP
  TITLE     A Ratiometric Dual Color Luciferase Reporter for Fast 
            Characterization of Transcriptional Regulatory Elements in Plants.
  JOURNAL   ACS Synth Biol. 2021 Sep 14. doi: 10.1021/acssynbio.1c00248.
  PUBMED    34520169
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2021 Sep 14. doi: 10.1021/acssynbio.1c00248."
COMMENT     Sequence Label: pUPD2+NtEUt
FEATURES             Location/Qualifiers
     source          1..2595
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     297..316
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     544..562
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(566..609)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    612..633
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      1180..1237
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1261..1278)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1432..2020)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1512..1531)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2203..2297)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2318..2595,1..382))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt
       61 cactccagag tgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa
      121 cactatccca tatcaccagc tcaccgtctt tcattgccat acgaaattcc ggatgagcat
      181 tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta
      241 cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa
      301 ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat
      361 atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa
      421 aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc
      481 cgatcaactc gagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
      541 aaaaataggc gtatcacgag gcagaatttc agataaaaaa aatccttagc tttcgctaag
      601 gatgatttct ggaattcgcg gccgcttcta gagcgatgag tggtctcggc ttaaagcaga
      661 atgctgagct aaaagaaagg ctttttccat tttcgagaga caatgagaaa agaagaagaa
      721 gaagaagaag aagaagaaga agaagaagaa aagagtaaat aataaagccc cacaggaggc
      781 gaagttcttg tagctccatg ttatctaagt tattgatatt gtttgcccta tattttattt
      841 ctgtcattgt gtatgttttg ttcagtttcg atctccttgc aaaatgcaga gattatgaga
      901 tgaataaact aagttatatt attatacgtg ttaatattct cctcctctct ctagctagcc
      961 ttttgttttc tctttttctt atttgatttt ctttaaatca atccatttta ggagagggcc
     1021 agggagtgat ccagcaaaac atgaagatta gaagaaactt ccctcttttt tttcctgaaa
     1081 acaatttaac gtcgagattt atctcttttt gtaatggaat catttctaca gttatgaccg
     1141 cttgagacca ctcatcgcga ctagtagcgg ccgctgcagt ccggcaaaaa agggcaaggt
     1201 gtcaccaccc tgcccttttt ctttaaaacc gaaaagatta cttcgcgtta tgcaggcttc
     1261 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     1321 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     1381 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccacag
     1441 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     1501 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     1561 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     1621 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     1681 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     1741 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     1801 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     1861 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     1921 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     1981 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     2041 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     2101 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     2161 aagtatatat gagtaaactt ggtctgacag ctcgaggctt ggattctcac caataaaaaa
     2221 cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg
     2281 atctatcaac aggagtccaa gcgagctcga tatcaaatta cgccccgccc tgccactcgt
     2341 cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat
     2401 gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca
     2461 tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga
     2521 aactcaccca gggattggct gatacgaaaa acatattctc aataaaccct ttagggaaat
     2581 aggccaggtt ttcac
//
