LOCUS       sequence-33442-e        1004 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Radoshevich L, Murrow L, Chen N, Fernandez E, Roy S, Fung C, Debnath
            J
  TITLE     ATG12 conjugation to ATG3 regulates mitochondrial homeostasis and 
            cell death.
  JOURNAL   Cell. 2010 Aug 20. 142(4):590-600.
  PUBMED    20723759
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2010 
            Aug 20. 142(4):590-600."
COMMENT     Sequence Label: pBABEpuro KR ATG3
FEATURES             Location/Qualifiers
     source          1..1004
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(11..28)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(38..79)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 cgattccgtc atggtgatgg tgatgatgac cggtacgcgt agaatcgaga ccgaggagag
       61 ggttagggat aggcttacct tcgaaccgcg ggccctctag actcgagcgg ccgcaaatca
      121 ttgtgaagtg tcttgtgtag tcatattcta ttgttggaat gacagcttga acaaatttca
      181 aaaaaattaa aagatacata tgaacaccaa gctctccccc gccttctgca actgtctcaa
      241 taattttctt catcacttca gcatgcctgc atgggtgaac tgaacacata ggaggtggtg
      301 ggagatgagg atggttttca atggtcactg ttctcttcac atgatcttga ctgatgtctt
      361 catacatgtg ctcaactgtt aaaggctgcc gttgctcatc atagccaaac aaccatagcc
      421 gtggtgtctg gtaatatttg tcgtaagtga tgtacagatc gtatgttctc gtttgtaaaa
      481 tagcatcttc acctccagcg tcagccttag ctttgcaggc ttccactatt ttccttgtgt
      541 ctagggtagc ctcatctgtt tccaacaatc cactctcttc atattcttcc atgtctgcag
      601 cttccccttc atcttcctcg tcttcttcat cacacagtgc tgagcaatct tggagtttta
      661 tactgtcctt gctttccagt gtaatctcct taactgcttc agtaattcct gtaatacctg
      721 tgttgtgata tgtatctacc catcccccat caccatcatc ttcttcaatg atagcttcca
      781 attcatccga atactccatc tgtttacacc gcttgtagca tggaacattt ttggttacca
      841 aaaattgttt gtctgtcgga agatatgcct tcactttcaa ttcttcccct gtagcccatt
      901 gccatgttgg acagtggtgg actaagtgat ctccagctgc cacaaactct tctggagtga
      961 ttacacccgt ttccttaaat tttgattcct tgaggaccgg ggtc
//
