LOCUS       sequence-334425-        1128 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Streptomyces codon-optimised beta-D-glucuronidase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Moore SJ, Lai HE, Chee SM, Toh M, Coode S, Chengan K, Capel P, Corre
            C, de Los Santos EL, Freemont PS
  TITLE     A Streptomyces venezuelae Cell-Free Toolkit for Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2021 Feb 19;10(2):402-411. doi: 
            10.1021/acssynbio.0c00581. Epub 2021 Jan 26.
  PUBMED    33497199
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.0c00581"; journalName: "ACS Synth Biol"; date: 
            "2021-02-19- 19"; volume: "10"; issue: "2"; pages: "402-411"
COMMENT     Sequence Label: pTU1-B-SP44s-GUS
FEATURES             Location/Qualifiers
     source          1..1128
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     17..35
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(39..82)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    85..104
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
ORIGIN
        1 ttaggattac ctataaaata ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt
       61 agctttcgct aaggatgatt tctggaattc gcggccgctt ctagaacgtc tcatgcccta
      121 tttgacaaca tgctgtgcgg tgttgtaaag tgtactttaa ctttaagaag gagatataca
      181 tatgctgcgc ccggtcgaaa ccccgacgcg cgagatcaag aagctcgacg gcctctgggc
      241 cttctcgctc gaccgcgaga actgcggcat cgaccagcgg tggtgggaga gcgccctcca
      301 ggagtcccgc gcgatcgccg tgccgggctc cttcaacgac cagttcgccg acgccgacat
      361 ccgcaactac gccggcaacg tgtggtacca gcgcgaggtg ttcatcccga agggctgggc
      421 cggccagcgc atcgtcctcc gcttcgacgc ggtcacccac tacggcaagg tctgggtcaa
      481 caaccaggag gtcatggagc accagggcgg ctacaccccg ttcgaggccg acgtcacccc
      541 gtacgtgatc gccggcaagt ccgtccgcat caccgtctgc gtgaacaacg agctgaactg
      601 gcagacgatc ccgccgggca tggtcatcac cgacgagaac ggcaagaaga agcagtccta
      661 cttccacgac ttcttcaact acgcgggcat ccaccgctcc gtcatgctct acaccacgcc
      721 gaacacctgg gtcgacgaca tcacggtcgt cacccacgtc gcccaggact gcaaccacgc
      781 ctccgtcgac tggcaggtcg tcgccaacgg cgacgtcagc gtcgagctgc gggacgccga
      841 ccagcaggtc gtggcgaccg gccagggcac ctcgggcacc ctccaggtcg tcaacccgca
      901 cctctggcag cccggcgagg gctacctcta cgagctgtgc gtcaccgcca agtcgcagac
      961 cgagtgcgac atctacccgc tccgcgtcgg catccggtcc gtcgccgtca agggcgagca
     1021 gttcctcatc aacacaagcc gttctacttc accggcttcg gccggcacga ggaagcggac
     1081 ctccgcggca agggcttcga caacgtcctc atgatccacg accacgcg
//
