LOCUS       sequence-334428-         990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  StrepFlex MoClo vector for cloning in E. coli and Streptomyces 
            expression - equivalent of pTU1-A-RFP from EcoFlex (ACS Synth. Biol.
            2016, 5, 10, 1059–1069).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Moore SJ, Lai HE, Chee SM, Toh M, Coode S, Chengan K, Capel P, Corre
            C, de Los Santos EL, Freemont PS
  TITLE     A Streptomyces venezuelae Cell-Free Toolkit for Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2021 Feb 19;10(2):402-411. doi: 
            10.1021/acssynbio.0c00581. Epub 2021 Jan 26.
  PUBMED    33497199
REFERENCE   2  (bases 1 to 990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.0c00581"; journalName: "ACS Synth Biol"; date: 
            "2021-02-19- 19"; volume: "10"; issue: "2"; pages: "402-411"
COMMENT     Sequence Label: pSF1C-A-RFP
FEATURES             Location/Qualifiers
     source          1..990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      297..368
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      384..411
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(451..468)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(696..715)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gatgggagct tcaccgaacg tatgtacccg gaagacggtg ctctgaaagg tgaaatcaaa
       61 atgcgtctga aactgaaaga cggtggtcac tacgacgctg aagttaaaac cacctacatg
      121 gctaaaaaac cggttcagct gccgggtgct tacaaaaccg acatcaaact ggacatcacc
      181 tcccacaacg aagactacac catcgttgaa cagtacgaac gtgctgaagg tcgtcactcc
      241 accggtgctt aataacgctg atagtgctag tgtagatcgc tactagagcc aggcatcaaa
      301 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
      361 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttataggt
      421 ctcatgtttg ccagagacgc tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt
      481 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atttaagccg aacaggaagc
      541 acagctccta ctgaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
      601 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
      661 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      721 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
      781 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
      841 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
      901 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
      961 ggcagcagcc actggtaaca ggattagcag
//
