LOCUS       sequence-334434-         764 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  StrepFlex MoClo vector for E.coli cloning and Streptomyces 
            chromosomal integration - equivalent of pTU2-A-RFP from EcoFlex (ACS
            Synth. Biol. 2016, 5, 10, 1059–1069).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Moore SJ, Lai HE, Chee SM, Toh M, Coode S, Chengan K, Capel P, Corre
            C, de Los Santos EL, Freemont PS
  TITLE     A Streptomyces venezuelae Cell-Free Toolkit for Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2021 Feb 19;10(2):402-411. doi: 
            10.1021/acssynbio.0c00581. Epub 2021 Jan 26.
  PUBMED    33497199
REFERENCE   2  (bases 1 to 764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.0c00581"; journalName: "ACS Synth Biol"; date: 
            "2021-02-19- 19"; volume: "10"; issue: "2"; pages: "402-411"
COMMENT     Sequence Label: pSF2C-A-RFP
FEATURES             Location/Qualifiers
     source          1..764
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(49..76)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(92..163)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gtgagcgagg aagcctgcag ggtctctgta cctaatgaga cgtataaacg cagaaaggcc
       61 cacccgaagg tgagccagtg tgactctagt agagagcgtt caccgacaaa caacagataa
      121 aacgaaaggc ccagtctttc gactgagcct ttcgttttat ttgatgcctg gctctagtag
      181 cgatctacac tagcactatc agcgttatta agcaccggtg gagtgacgac cttcagcacg
      241 ttcgtactgt tcaacgatgg tgtagtcttc gttgtgggag gtgatgtcca gtttgatgtc
      301 ggttttgtaa gcacccggca gctgaaccgg ttttttagcc atgtaggtgg ttttaacttc
      361 agcgtcgtag tgaccaccgt ctttcagttt cagacgcatt ttgatttcac ctttcagagc
      421 accgtcttcc gggtacatac gttcggtgga agcttcccaa cccatggttt ttttctgcat
      481 aaccggaccg tcggacggga agttggtacc acgcagttta actttgtaga tgaactcacc
      541 gtcttgcagg gaggagtcct gggtaacggt aacaacacca ccgtcttcga agttcataac
      601 acgttcccat ttgaaacctt ccgggaagga cagtttcagg tagtccggga tgtcagccgg
      661 gtgtttaacg taagctttgg aaccgtactg gaactgcggg gacaggatgt cccaagcgaa
      721 cggcagcgga ccacctttgg taactttcag tttagcggtc tggg
//
