LOCUS       sequence-33444-e         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Radoshevich L, Murrow L, Chen N, Fernandez E, Roy S, Fung C, Debnath
            J
  TITLE     ATG12 conjugation to ATG3 regulates mitochondrial homeostasis and 
            cell death.
  JOURNAL   Cell. 2010 Aug 20. 142(4):590-600.
  PUBMED    20723759
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2010 
            Aug 20. 142(4):590-600."
COMMENT     Sequence Label: pMSCV FHA-Stop
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             64..87
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             100..126
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             100..126
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(638..661)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 ccctcactcc ttctctaggc gccggaatta gatctctcga gatcttccgc tcgatgaacc
       61 atggactaca aggacgacga tgacaagctc gatggaggat acccctacga cgtgcccgac
      121 tacgccggag gactcgagcg gatgtcggaa gattcagagg ttgtgctgca gctcccctcg
      181 gctcctgtag gagccggcgg cgagagcctt ccagagctct ccccggaaac agccacccca
      241 gagcccccgt cctcggctgc agtttcgccc ggaacggagg aacctcccgg agacaccaag
      301 aaaaaaattg acatcctgct gaaggctgta ggagacactc ctataatgaa aacaaagaaa
      361 tgggctgtgg agcgaacccg gaccatccaa ggactcattg acttcatcaa aaagttcctt
      421 aaactggtgg cctcggaaca gttgtttatt tatgtgaatc agtcctttgc cccttcccca
      481 gaccaagaag ttggaactct atatgagtgt tttggcagtg atggtaaact ggtcctgcat
      541 tactgcaaat cccaggcatg gtgatgaata gtttagcggc ctcgaggtta acgaattcta
      601 ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct
      661 gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag
      721 gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt
      781 caggaagttc ccccccgccc cgcagctcgc gtcgtg
//
