LOCUS       sequence-33445-e         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Poltorak A, He X, Smirnova I, Liu MY, Van Huffel C, Du X, Birdwell 
            D, Alejos E, Silva M, Galanos C, Freudenberg M, Ricciardi-Castagnoli
            P, Layton B, Beutler B
  TITLE     Defective LPS signaling in C3H/HeJ and C57BL/10ScCr mice: mutations 
            in Tlr4 gene.
  JOURNAL   Science. 1998 Dec 11. 282(5396):2085-8.
  PUBMED    9851930
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            1998 Dec 11. 282(5396):2085-8."
COMMENT     Sequence Label: p3xFLAG-CMV-Tlr4(Lps)
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             852..860
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 tccacttgcc cttgctccat accacccccc tccaccccat aatattatag aaggacacct
       61 agtcagacaa aatgatgcaa cttaatttta ttaggacaag gctggtgggc actggagtgg
      121 caacttccag ggccaggaga ggcactgggg aggggtcaca gggatgccac ccgggatcct
      181 ctagagtcga ctcaggtcca agttgccgtt tcttgttctt cctctgctgt ttgctcagga
      241 ttcgaggctt ttccatccaa tagggcattt ttaagtcttc tccagaagat gtgcctcccc
      301 agaggattgt cctcccattc caggtaggtg tttctgctaa gaaggcgata caattccacc
      361 tgctgcctca gcagggactt ctcaaccttc tcaaggacaa tgaagatgat gccagagcgg
      421 ctgctcagaa actgccatgt ttgagcaatc tcatattcaa agatacacca acggctctga
      481 ataaagtgtc tagacactac cacaataacc ttccggctct tgtggaagcc ttcctggatg
      541 atgttggcag caatggctac accatgaata aagtctctgt agtgaaggca gaggtgaaag
      601 cggggcactc cttcttctaa attctttacc agctcatttc tcacccagtc ctcattctga
      661 ctcgagtaga tcacaaatgc atcatagatg ctttctcctc tgctgtactt tttacagcca
      721 gcaataagta tcaggtgaaa atagaagtgg tatatcagaa atgctacagt ggataccaca
      781 atcacactga ccactgacac actgatgatt gtcttgtaca tataacaggt agaattatta
      841 aaatccaaca ctaaggaggt attcatctct acaggtgttg cacatgtca
//
