LOCUS       sequence-334454-        9108 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpresses GABRA5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9108)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 9108)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_GABRA5_OE_1
FEATURES             Location/Qualifiers
     source          1..9108
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        16..319
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        320..523
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     474..494
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    546..570
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1957..1981)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1983..2024
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2066..2654
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2119..2139)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2537..2548)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2726..2959
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3037..3158
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3074..3093)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3128..3147
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3198..3333
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3353..3372)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3354..3372)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3382..3399)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3382..3398)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3391..3413)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3540..3995
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3627..3646)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3837..3858
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4021..4125
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4126..4194,4195..4986)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4344..4363)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5157..5745
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5646..5665
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5899..5916
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6033..6054
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6069..6074,6075..6092,6093..6099)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6107..6123
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6112..6134
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6131..6147
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6131..6147
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6166..6186
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6168..6186
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6214..6440
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6441..6621
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6668..6793
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7286..7519
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7704..7748
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7923..8423
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8342..8360
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8435..8830
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8890..9007
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
       61 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
      121 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
      181 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
      241 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
      301 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
      361 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
      421 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
      481 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc taactgtcgg
      541 gatcaacaag tttgtacaaa aaagttggca tggagaatgg aatgttctct ggttttatca
      601 tgatcaaaaa cctccttctc ttttgtattt ccatgaactt atccagtcac tttggctttt
      661 cacagatgcc aaccagttca gtgaaagatg agaccaatga caacatcacg atatttacca
      721 ggatcttgga tgggctcttg gatggctacg acaacagact tcggcccggg ctgggagagc
      781 gcatcactca ggtgaggacc gacatctacg tcaccagctt cggcccggtg tccgacacgg
      841 aaatggagta caccatagac gtgtttttcc gacaaagctg gaaagatgaa aggcttcggt
      901 ttaaggggcc catgcagcgc ctccctctca acaacctcct tgccagcaag atctggaccc
      961 cagacacgtt cttccacaac gggaagaagt ccatcgctca caacatgacc acgcccaaca
     1021 agctgctgcg gctggaggac gacggcaccc tgctctacac catgcgcttg accatctctg
     1081 cagagtgccc catgcagctt gaggacttcc cgatggatgc gcacgcttgc cctctgaaat
     1141 ttggcagcta tgcgtaccct aattctgaag tcgtctacgt ctggaccaac ggctccacca
     1201 agtcggtggt ggtggcggaa gatggctcca gactgaacca gtaccacctg atggggcaga
     1261 cggtgggcac tgagaacatc agcaccagca caggcgaata cacaatcatg acagctcact
     1321 tccacctgaa aaggaagatt ggctactttg tcatccagac ctaccttccc tgcataatga
     1381 ccgtgatctt atcacaggtg tccttttggc tgaaccggga atcagtccca gccaggacag
     1441 tttttggggt caccacggtg ctgaccatga cgaccctcag catcagcgcc aggaactctc
     1501 tgcccaaagt ggcctacgcc accgccatgg actggttcat agccgtgtgc tatgccttcg
     1561 tcttctcggc gctgatagag tttgccacgg tcaattactt taccaagaga ggctgggcct
     1621 gggatggcaa aaaagccttg gaagcagcca agatcaagaa aaagcgtgaa gtcatactaa
     1681 ataagtcaac aaacgctttt acaactggga agatgtctca ccccccaaac attccgaagg
     1741 aacagacccc agcagggacg tcgaatacaa cctcagtctc agtaaaaccc tctgaagaga
     1801 agacttctga aagcaaaaag acttacaaca gtatcagcaa aattgacaaa atgtcccgaa
     1861 tcgtattccc agtcttgttc ggcactttca acttagttta ctgggcaacg tatttgaata
     1921 gggagccggt gataaaagga gccgcctctt caaaataccc aactttcttg tacaaagtgg
     1981 ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg
     2041 ctaaccggtg gcgcgttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt
     2101 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     2161 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     2221 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     2281 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     2341 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     2401 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     2461 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     2521 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     2581 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     2641 ggccgcctcc ccgcgtcgac tttaagacca atgacttaca aggcagctgt agatcttagc
     2701 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     2761 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     2821 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     2881 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     2941 gtgtggaaaa tctctagcag tacgtatagt agttcatgtc atcttattat tcagtattta
     3001 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat
     3061 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     3121 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc
     3181 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     3241 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     3301 tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag
     3361 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     3421 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     3481 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga
     3541 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     3601 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     3661 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     3721 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     3781 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     3841 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     3901 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     3961 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg
     4021 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     4081 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     4141 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     4201 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     4261 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     4321 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     4381 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     4441 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     4501 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     4561 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     4621 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     4681 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     4741 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     4801 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     4861 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     4921 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     4981 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     5041 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     5101 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     5161 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     5221 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     5281 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     5341 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     5401 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     5461 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     5521 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     5581 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     5641 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     5701 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     5761 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     5821 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     5881 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     5941 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     6001 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     6061 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     6121 caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac
     6181 taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt
     6241 cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg
     6301 catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg
     6361 gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt
     6421 gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct
     6481 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     6541 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     6601 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
     6661 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
     6721 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
     6781 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
     6841 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
     6901 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
     6961 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
     7021 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
     7081 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga
     7141 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
     7201 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
     7261 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
     7321 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
     7381 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
     7441 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
     7501 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
     7561 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
     7621 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
     7681 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
     7741 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
     7801 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
     7861 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
     7921 ccttgcgcct tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg
     7981 gttccgggaa acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca
     8041 gcgtcacccg gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc
     8101 ccctaagtcg ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg
     8161 cacgtctcac tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac
     8221 cgcgatgggc tgtggccaat agcggctgct cagcagggcg cgccgagagc agcggccggg
     8281 aaggggcggt gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc
     8341 ggtgttccgc attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc
     8401 gaatcaccga cctctctccc cagggggtac caccatggcc aagcctttgt ctcaagaaga
     8461 atccaccctc attgaaagag caacggctac aatcaacagc atccccatct ctgaagacta
     8521 cagcgtcgcc agcgcagctc tctctagcga cggccgcatc ttcactggtg tcaatgtata
     8581 tcattttact gggggacctt gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc
     8641 agctggcaac ctgacttgta tcgtcgcgat cggaaatgag aacaggggca tcttgagccc
     8701 ctgcggacgg tgccgacagg tgcttctcga tctgcatcct gggatcaaag ccatagtgaa
     8761 ggacagtgat ggacagccga cggcagttgg gattcgtgaa ttgctgccct ctggttatgt
     8821 gtgggagggc ctgcagctgc agtagtaaga attctagatc ttgagacaaa tggcagtatt
     8881 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     8941 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     9001 aaattttcgg gtttattaca gggacagcag agatccactt tggcgccggc tcgagggggc
     9061 ccggggttat taatagtaat caattacggg gtcattagtt catagccc
//
