LOCUS       sequence-334459-        6533 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  DEPRECATED: Please see Addgene item #169249 (coming soon) for an 
            improved version of this plasmid. AAV transfer plasmid for neuronal 
            expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8f..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6533)
  AUTHORS   Grodem S, Nymoen I, Vatne GH, Rogge FS, Bjornsdottir V, Lensjo KK, 
            Fyhn M
  TITLE     An updated suite of viral vectors for in vivo calcium imaging using 
            intracerebral and retro-orbital injections in male mice.
  JOURNAL   Nat Commun. 2023 Feb 4;14(1):608. doi: 10.1038/s41467-023-36324-3.
  PUBMED    36739289
REFERENCE   2  (bases 1 to 6533)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-02-4"; volume: "14(1):608. doi"; pages: " 
            10.1038/s41467-023-36324-3"
COMMENT     Sequence Label: AAV-hSyn-Ribo-jGCaMP8f
FEATURES             Location/Qualifiers
     source          1..6533
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        183..630
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             667..684
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     966..987
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1093..1114)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1354..1373)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    2660..3248
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2713..2733)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3131..3142)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3280..3756
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3479..3498)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3796..3936
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3796..3925
                     /label=AAV2 ITR
     rep_origin      4011..4466
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4098..4117)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4308..4329
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4483..4502)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4602..4624
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4662..4680)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4748..4852
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4853..4921,4922..5713)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5071..5090)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5884..6472
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6373..6392
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtttaatt aagtgtctag actgcagagg gccctgcgta
      181 tgagtgcaag tgggttttag gaccaggatg aggcggggtg ggggtgccta cctgacgacc
      241 gaccccgacc cactggacaa gcacccaacc cccattcccc aaattgcgca tcccctatca
      301 gagaggggga ggggaaacag gatgcggcga ggctcgtgcg cactgccagc ttcagcaccg
      361 cggacagtgc cttcgccccc gcctggcggc gcgcgccacc gccgcctcag cactgaaggc
      421 gcgctgacgt cactcgccgg tcccccgcaa actccccttc ccggccacct tggtcgcgtc
      481 cgcgccgccg ccggcccagc cggaccgcac cacgcgaggc gcgagatagg ggggcacggg
      541 cgcgaccatc tgcgctgcgg cgccggcgac tcagcgctgc ctcagtctgc ggtgggcagc
      601 ggaggagtcg tgtcgtgcct gagagcgcag tcgagaaacc ggctagagga tccacccgcc
      661 accatgcatc atcaccatca tcacacgcgt cgcaagaaga ccttcaagga ggtggccacc
      721 gccgtgaaga tcatcgcccg cctgatgggc ctgaagatca acgtctatat caaggccgac
      781 aagcagaaga acggcatcaa ggcgaacttc cacatccgcc acaacatcga ggacggcggc
      841 gtgcagctcg cctaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
      901 cccgacaacc actacctgag cgtggagtcc aaactttcga aagaccccaa cgagaagcgc
      961 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1021 ctgtacaagg gcggtaccgg agggagcatg gtgagcaagg gcgaggagct gttcaccggg
     1081 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     1141 ggcgagggtg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     1201 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     1261 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     1321 ggctacatcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     1381 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     1441 aaggaggacg gcaacatcct ggggcacaag ctggagtaca acctgccgga ccaactgact
     1501 gaagagcaga tcgcagaatt taaagaggct ttctccctat ttgacaagga cggggatggg
     1561 acaataacaa ccaaggagct ggggacggtg atgcggtctc tcggactgaa ccccacagaa
     1621 gcagagctgc aggacatgat caatgaagta gatgccgacg gtgacggcac aatcgacttc
     1681 cctgagttcc tgacaatgat ggcaagaaaa atgaaataca gggacacgga agaagaaatt
     1741 agagaagcgt tcggtgtgtt tgataaggat ggcaatggct acatcagtgc agcagagctt
     1801 cgccacgtga tgacaaacct tggagagaag ttaacagatg aagaggttga tgaaatgatc
     1861 agggaagcag acatcgatgg ggatggtcag gtaaactacg aagagtttgt acaaatgatg
     1921 acagcgaagt caggccggac tcagatctca agctcaagct tcgagttcag cagcaaagtc
     1981 tcacgcgaca ccctgtacga ggcggtgcgg gaagtcctgc acgggaacca gcgcaagcgc
     2041 cgcaagtttc tggagacggt ggagctgcag atcagcctga agaactacga ccctcagaag
     2101 gacaaacgtt tctcgggcac cgtcaggctc aagtccaccc cacgccccaa gttctcggtg
     2161 tgcgttctgg gggaccagca gcactgtgat gaagccaagg ccgtggatat cccccacatg
     2221 gacatcgagg cgctcaagaa acttaacaaa aacaagaagt tggtcaagaa gctggctaag
     2281 aagtacgatg cctttttggc ctctgagtct ctgattaagc agatcccacg tatcctgggc
     2341 ccaggcctaa acaaggctgg caagttcccc tccctgctga cacacaatga aaacatggtg
     2401 gccaaagtgg atgaggtgaa atcgacaatc aagttccaga tgaagaaggt gctgtgtttg
     2461 gccgtcgctg ttggccacgt gaagatgacc gatgatgagc tagtctacaa cattcatctg
     2521 gctgtcaatt tcttggtgtc cttgcttaag aaaaactggc aaaacgtgcg ggctctgtac
     2581 atcaagagca ccatgggcaa gccccagcgt ctgtattagg gcgcgcccct gcagggaatt
     2641 cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
     2701 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     2761 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     2821 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     2881 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     2941 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     3001 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     3061 gtcctttcct tggctgctcg cctatgttgc cacctggatt ctgcgcggga cgtccttctg
     3121 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     3181 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     3241 ctccccgcat cgataccgag cgctgctcga gagatctacg ggtggcatcc ctgtgacccc
     3301 tccccagtgc ctctcctggc cctggaagtt gccactccag tgcccaccag ccttgtccta
     3361 ataaaattaa gttgcatcat tttgtctgac taggtgtcct tctataatat tatggggtgg
     3421 aggggggtgg tatggagcaa ggggcaagtt gggaagacaa cctgtagggc ctgcggggtc
     3481 tattgggaac caagctggag tgcagtggca caatcttggc tcactgcaat ctccgcctcc
     3541 tgggttcaag cgattctcct gcctcagcct cccgagttgt tgggattcca ggcatgcatg
     3601 accaggctca gctaattttt gtttttttgg tagagacggg gtttcaccat attggccagg
     3661 ctggtctcca actcctaatc tcaggtgatc tacccacctt ggcctcccaa attgctggga
     3721 ttacaggcgt gaaccactgc tcccttccct gtccttctga ttttgtaggt aaccacgtgc
     3781 ggaccgagcg gccgcaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc
     3841 gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg
     3901 cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc
     3961 ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg
     4021 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     4081 cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     4141 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     4201 gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg
     4261 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     4321 ccaaactgga acaacactca actctatctc gggctattct tttgatttat aagggatttt
     4381 gccgatttcg gtctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     4441 taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc
     4501 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     4561 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     4621 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
     4681 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     4741 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     4801 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     4861 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     4921 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     4981 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     5041 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     5101 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     5161 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     5221 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     5281 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     5341 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     5401 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     5461 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     5521 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     5581 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     5641 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     5701 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     5761 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     5821 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     5881 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     5941 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     6001 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     6061 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     6121 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     6181 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     6241 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     6301 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     6361 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     6421 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     6481 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgt
//
