LOCUS       sequence-334468-        7965 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GoldenBraid Double Transcriptional Unit - 
            pCmYLCV911_E-Luc_NtEUt+pNOS_Red-F_NOSt.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7965)
  AUTHORS   Gonzalez-Grandio E, Demirer GS, Ma W, Brady S, Landry MP
  TITLE     A Ratiometric Dual Color Luciferase Reporter for Fast 
            Characterization of Transcriptional Regulatory Elements in Plants.
  JOURNAL   ACS Synth Biol. 2021 Sep 14. doi: 10.1021/acssynbio.1c00248.
  PUBMED    34520169
REFERENCE   2  (bases 1 to 7965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2021 Sep 14. doi: 10.1021/acssynbio.1c00248."
COMMENT     Sequence Label: pGREAT10
FEATURES             Location/Qualifiers
     source          1..7965
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             1960..1968
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     promoter        2697..2876
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     terminator      4581..4833
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4868..4886)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    5075..5099
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(5190..5778)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5270..5289)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5949..6740)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7384..7819
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    join(7946..7965,1..3)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
ORIGIN
        1 taacggatcc ggtctcagga gtggcagaca tactgtccca caaatgaaga tggaatctgt
       61 aaaagaaaac gcgtgaaata atgcgtctga caaaggttag gtcggctgcc tttaatcaat
      121 accaaagtgg tccctaccac gatggaaaaa ctgtgcagtc ggtttggctt tttctgacga
      181 acaaataaga ttcgtggccg acaggtgggg gtccaccatg tgaaggcatc ttcagactcc
      241 aataatggag caatgacgta agggcttacg aaataagtaa gggtagtttg ggaaatgtcc
      301 actcacccgt cagtctataa atacttagcc cctccctcat tgttaaggga gcaaaatctc
      361 agagagatag tcctagagag agaaagagag caagtagcct agaagtagtc aaggcggcga
      421 agtattcagg caggtggcca ggaagaagaa aagccaagac gacgaaaaca ggtaagagct
      481 aagcttaatg gagagagaga agaacgtggt gtacggcccc gagcccaagc accctctggg
      541 caacttcacc gccggcgaga tgctgtacaa cgctctgcac aagcactccc acatccccca
      601 ggccatcctg gacgtgatgg gcaacgagtc cctttcctac caggagttct tcgacactac
      661 tgtgaagctg ggccagagcc tccagaactg tggctacaag atgaacgatg tcgtgtcgat
      721 ctgtgcagag aacaacaaga gattcttcat ccccatcatc tccgcctggt acatcggcat
      781 ggtggtggcc cctgtgaacg aggactatat cccagacgag ctgtgtaaag tgaccggcat
      841 ctccaagccg atcctggtct ttaccactag gaagatcctg cctaaggttt tggaggttaa
      901 agacagaacc aactacataa agaggatcat catactggac tctgaagaga acctgctggg
      961 ctgcgagagc ctgcacaact tcatgtccag gtactccgac aacaacctcc aaacattcaa
     1021 gcctctgcac tacgaccctg tggaccaggt agccgccatc ctgtgctcct ccggcacaac
     1081 cggcctgcct aaaggcgtga tgcagaccca caggaacatc tgtgtgagac tcacacacgc
     1141 atctgacccc agagtgggta cacaactcat ccccggcgta tccgtgctgg cctacctgcc
     1201 attcttccac gccttcggct tcagtatcaa cctgggctat ttcatggtgg gcctgagagt
     1261 ggtgatgctc cgaaggttta accaggaggt gttcctgaag gccatccagg actacgaggt
     1321 gaggagcgtg atcaacgttc cctccacaat cctgttcctg tccaagagcc ctctggtgga
     1381 caagtacgac ctatccaccc tggcggagct gtgctgtgga gccgctcctc tggcgaagga
     1441 ggtggccgag atcgccgtga agaggctgaa cctgccaggg atacggtgtg gctacggtct
     1501 aacagagtct acctccgcca acatccatac tctgcacaac gagttcaagt ccggctccct
     1561 gggcaaggtg acaccttaca tggccgccaa gatcatcgac aggaacaccg gcgaggccct
     1621 gggtccaaac caggtgggcg agctgtgcat ctggggacct atggtaacaa aaggctatgt
     1681 gaacaaccca caggctacta aggaggccat cgacgacgac ggctggctgc actctggcga
     1741 cttcggctac tacgacgagg acgagtattt ctacatcgtg gaccggtaca aggagctgat
     1801 caaatacaag ggctatcagg tcgcccctgt ggagctggag gagatcctcc ttcagcaccc
     1861 aggcatcagg gacgtggccg tcgtgggtat ccctgacatc gaggccggcg agctgccagc
     1921 cggcttcgtg gtgaagcagc ccggcgccca actcaccgct aaggaggtgt acgacttcct
     1981 ggcccagagg gtgtctcact ccaagtacct gaggggcggc gtaaggttcg tggactctat
     2041 ccccaggaac gtgacaggca agattagtcg aaaagagctg agggaggccc tgatggagaa
     2101 ggcttctaag ctgtaagctt aaagcagaat gctgagctaa aagaaaggct ttttccattt
     2161 tcgagagaca atgagaaaag aagaagaaga agaagaagaa gaagaagaag aagaagaaaa
     2221 gagtaaataa taaagcccca caggaggcga agttcttgta gctccatgtt atctaagtta
     2281 ttgatattgt ttgccctata ttttatttct gtcattgtgt atgttttgtt cagtttcgat
     2341 ctccttgcaa aatgcagaga ttatgagatg aataaactaa gttatattat tatacgtgtt
     2401 aatattctcc tcctctctct agctagcctt ttgttttctc tttttcttat ttgattttct
     2461 ttaaatcaat ccattttagg agagggccag ggagtgatcc agcaaaacat gaagattaga
     2521 agaaacttcc ctcttttttt tcctgaaaac aatttaacgt cgagatttat ctctttttgt
     2581 aatggaatca tttctacagt tatgaccgct gtcaggagat acatgagaat taagggagtc
     2641 acggtatgac ccccgccgat gacgcgggac aagccgtttt acgtttggaa ctgacagaac
     2701 cgcaacgttg aaggagccac tgagccgcgg gtttctggag tttaatgagc taagcacata
     2761 cgtcagaaac cattattgcg cgttcaaaag tcgcctaagg tcactatcag ctagcaaata
     2821 tttcttgtca aaaatgctcc actgacgttc cataaattcc cctcggtatc caattagagt
     2881 ctcatattca ctctcaactc gatcgaggca atggagaaca tggagaacga cgagaacatt
     2941 gtggtgggac ccaagccgtt ctacccgatc gaggagggtt ccgctggcac ccagctgcgc
     3001 aagtacatgg agcgctacgc taagctgggt gctatcgcct tcaccaacgc cgtgaccggt
     3061 gtggattact cctacgccga gtacctggag aagtcctgct gcctcggaaa ggccctgcag
     3121 aactacggtc tggtcgtgga tggccgcatt gccctctgct cggagaactg cgaggagttc
     3181 ttcattcccg tgatcgctgg tctgttcatc ggagtcggcg ttgccccaac caacgagatc
     3241 tacaccctgc gcgagctggt ccacagcctg ggtatcagca agccgaccat cgttttctcg
     3301 agcaagaagg gcctcgacaa ggttatcacc gtgcaaaaaa ccgtcaccac catcaagacc
     3361 atcgttatcc tggattccaa ggttgactac cgcggttacc agtgcctcga caccttcatc
     3421 aagcgtaaca ccccacccgg cttccaagcc tcctccttca aaaccgtgga ggttgatcgc
     3481 aaggagcagg tggccctgat catgaactcc tccggctcca ccggtctgcc aaagggcgtg
     3541 cagctgaccc acgagaacac cgtcacccgc ttctcgcatg cccgtgatcc catctacggc
     3601 aaccaggtgt ccccaggcac cgccgtcctg accgtcgtgc ccctccacca cggcttcggc
     3661 atgttcacca ctctcggcta cttgatctgc ggcttccgtg tggtgatgct gactaagttc
     3721 gacgaggaga ctttcctcaa gaccctgcaa gactacaagt gcacttacgt gatcctggtg
     3781 ccgaccctgt tcgccatcct gaacaagagc gagctgttga acaaatacga cctgtcgaac
     3841 ctggtggaga tcgcttccgg cggcgcccca ctgtcgaagg aagttggcga ggccgttgct
     3901 cgccgcttca acctgcccgg cgtccgtcag ggttacggcc tgaccgagac tacctcggct
     3961 atcatcatca cccccgaggg cgacgataag cccggcgctt ccggcaaggt tgttccactc
     4021 ttcaaagcca aggtgatcga cctggacacc aagaagtccc tgggtcccaa ccgtcgcgga
     4081 gaagtgtgcg tcaagggccc catgctgatg aagggctacg tcaacaaccc agaggccacc
     4141 aaggagttga tcgacgagga gggctggctg cacactggtg acatcggtta ctacgacgaa
     4201 gagaagcact tcttcatcgt tgaccgcctg aagtccttga tcaagtacaa gggctaccag
     4261 gttccccccg ccgagctcga gagcgtcctg ctgcagcacc cctccatttt cgatgccgga
     4321 gtggccggtg tccccgatcc cgtggctggc gagctgccag gtgccgtcgt cgtgctggag
     4381 tccggcaaga acatgaccga gaaggaagtg atggattacg tggcctcgca agtgtccaac
     4441 gccaagcgcc tgcgcggcgg cgtgcgcttc gtggatgagg tgcccaaggg cctcaccgga
     4501 aagatcgacg gtcgcgctat ccgcgagatc ctcaagaagc ccgtggccaa gatgtgagct
     4561 tggaatggat cttcgatccc gatcgttcaa acatttggca ataaagtttc ttaagattga
     4621 atcctgttgc cggtcttgcg acgattatca tataatttct gttgaattac gttaagcatg
     4681 taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc
     4741 cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat
     4801 tatcgcgcgc ggtgtcatct atgttactag atcgggaatt gccaagctaa ttcttgaaga
     4861 tgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     4921 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     4981 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     5041 tgggaccgac tcgcgctgtc atgagaccgg atcctgacag gatatattgg cgggtaaact
     5101 aagtcgctgt atgtgtttgt ttgagatctc atgtgagcaa aaggccagca aaaggccagg
     5161 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     5221 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     5281 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     5341 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     5401 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     5461 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     5521 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     5581 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     5641 ggtatctgcg ctctgctgaa gccagttacc ttcggaagaa gagttggtag ctcttgatcc
     5701 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     5761 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     5821 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     5881 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatgt gtaacattgg
     5941 tctagtgatt atttgccgac taccttggtg atctcgcctt tcacgtagtg gacaaattct
     6001 tccaactgat ctgcgcgcga ggccaagcga tcttcttctt gtccaagata agcctgtcta
     6061 gcttcaagta tgacgggctg atactgggcc ggcaggcgct ccattgccca gtcggcagcg
     6121 acatccttcg gcgcgatttt gccggttact gcgctgtacc aaatgcggga caacgtaagc
     6181 actacatttc gctcatcacc agcccagtcg ggcggcgagt tccatagcgt taaggtttca
     6241 tttagcgcct caaatagatc ctgttcagga accggatcaa agagttcctc cgccgctgga
     6301 cctaccaagg caacgctatg ttctcttgct tttgtcagca agatagccag atcaatgtcg
     6361 atcgtggctg gctcgaagat acctgcaaga atgtcattgc gctgccattc tccaaattgc
     6421 agttcgcgct tagctggata acgccacgga atgatgtcgt cgtgcacaac aatggtgact
     6481 tctacagcgc ggagaatctc gctctctcca ggggaagccg aagtttccaa aaggtcgttg
     6541 atcaaagctc gccgcgttgt ttcatcaagc cttacggtca ccgtaaccag caaatcaata
     6601 tcactgtgtg gcttcaggcc gccatccact gcggagccgt acaaatgtac ggccagcaac
     6661 gtcggttcga gatggcgctc gatgacgcca actacctctg atagttgagt cgatacttcg
     6721 gcgatcaccg cttccctcat aatgtttaac tttgttttag ggcgactgcc ctgctgcgta
     6781 acatcgttgc tgctccataa catcaaacat cgacccacgg cgtaacgcgc ttgctgcttg
     6841 gatgcccgag gcatagactg taccccaaaa aaacagtcat aacaagccat gaaaaccgcc
     6901 actgcgccgt taccaccgct gcgttcggtc aaggttctgg accagttgcg tgagcgcata
     6961 cgctacttgc attacagctt acgaaccgaa caggcttatg tccactgggt tcgtgccttc
     7021 atccgtttcc acggtgtgcg tcacccggca accttgggta gcagcgaagt cgaggcattt
     7081 ctgtcctggc tggaacaccc cttgtattac tgtttatgta agcagacagt tttattgttc
     7141 atgatgatat atttttatct tgtgcaatgt aacatcagag attttgagac acaacgtggc
     7201 tttgttgaat aaatcgaact tttgctgagt tgaaggatca gatcacgcat cttcccgaca
     7261 acgcagaccg ttccgtggca aagcaaaagt tcaaaatcac caactggtcc acctacaaca
     7321 aagctctcat caaccgtggc tccctcactt tctggctgga tgatggggcg attcaggcga
     7381 tccccatcca acagcccgcc gtcgagcggg cttttttatc cccggaagcc tgtggataga
     7441 gggtagttat ccacgtgaaa ccgctaatgc cccgcaaagc cttgattcac ggggctttcc
     7501 ggcccgctcc aaaaactatc cacgtgaaat cgctaatcag ggtacgtgaa atcgctaatc
     7561 ggagtacgtg aaatcgctaa taaggtcacg tgaaatcgct aatcaaaaag gcacgtgaga
     7621 acgctaatag ccctttcaga tcaacagctt gcaaacaccc ctcgctccgg caagtagtta
     7681 cagcaagtag tatgttcaat tagcttttca attatgaata tatatatcaa ttattggtcg
     7741 cccttggctt gtggacaatg cgctacgcgc accggctccg cccgtggaca accgcaagcg
     7801 gttgcccacc gtcgagcgcc tttgcccaca acccggcggc cggccgcaac agatcgtttt
     7861 ataaattttt ttttttgaaa aagaaaaagc ccgaaaggcg gcaacctctc gggcttctgg
     7921 atttccgatc cccggaatta gatcttggca ggatatattg tggtg
//
