LOCUS       sequence-33447-e         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Poltorak A, He X, Smirnova I, Liu MY, Van Huffel C, Du X, Birdwell 
            D, Alejos E, Silva M, Galanos C, Freudenberg M, Ricciardi-Castagnoli
            P, Layton B, Beutler B
  TITLE     Defective LPS signaling in C3H/HeJ and C57BL/10ScCr mice: mutations 
            in Tlr4 gene.
  JOURNAL   Science. 1998 Dec 11. 282(5396):2085-8.
  PUBMED    9851930
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            1998 Dec 11. 282(5396):2085-8."
COMMENT     Sequence Label: p3xFLAG-CMV-Tlr4
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             33..77
                     /codon_start=1
                     /product="leader sequence from mouse preprotrypsin"
                     /label=preprotrypsin leader
                     /translation="MSALLILALVGAAVA"
     CDS             78..143
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 ctcgtttagt gaaccgtcag aattaattca ccatgtctgc acttctgatc ctagctcttg
       61 ttggagctgc agttgctgac tacaaagacc atgacggtga ttataaagat catgacatcg
      121 attacaagga tgacgatgac aagcttgcgg ccgcattgaa tccctgcata gaggtagttc
      181 ctaatattac ctaccaatgc atggatcaga aactcagcaa agtccctgat gacattcctt
      241 cttcaaccaa gaacatagat ctgagcttca accccttgaa gatcttaaaa agctatagct
      301 tctccaattt ttcagaactt cagtggctgg atttatccag gtgtgaaatt gaaacaattg
      361 aagacaaggc atggcatggc ttacaccacc tctcaaactt gatactgaca ggaaacccta
      421 tccagagttt ttccccagga agtttctctg gactaacaag tttagagaat ctggtggctg
      481 tggagacaaa attggcctct ctagaaagct tccctattgg acagcttata accttaaaga
      541 aactcaatgt ggctcacaat tttatacatt cctgtaagtt acctgcatat ttttccaatc
      601 tgacgaacct agtacatgtg gatctttctt ataactatat tcaaactatt actgtcaacg
      661 acttacagtt tctacgtgaa aatccacaag tcaatctctc tttagacatg tctttgaacc
      721 caattgactt cattcaagac caagcctttc agggaattaa gctccatgaa ctgactctaa
      781 ga
//
