LOCUS       sequence-334481-        8308 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encoding membrane-anchored tdTomato..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8308)
  AUTHORS   Ichise H, Tsukamoto S, Hirashima T, Konishi Y, Oki C, Tsukiji S, 
            Iwano S, Miyawaki A, Sumiyama K, Terai K, Matsuda M
  TITLE     Functional visualization of NK Cell-mediated killing of metastatic 
            single tumor cells.
  JOURNAL   Elife. 2022 Feb 3;11. pii: 76269. doi: 10.7554/eLife.76269.
  PUBMED    35113018
REFERENCE   2  (bases 1 to 8308)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-02-3"; volume: "11. pii: 76269. doi"; pages: " 
            10.7554/eLife.76269"
COMMENT     Sequence Label: pT2Aneo-tdTomato-CAAX
FEATURES             Location/Qualifiers
     source          1..8308
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    84..633
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(228..245)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     455..474
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             636..1430
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(690..709)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1300..1319
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             1437..1457
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1508..1642
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1532..1551)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1586..1605
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(1764..1926,1927..1938)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(1968..1987)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1969..1987)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1997..2014)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1997..2013)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2006..2028)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2155..2609
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2241..2260)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2451..2472
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2635..2739
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2740..2808,2809..3600)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2958..2977)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3771..4359
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4260..4279
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4513..4530
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4647..4668
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4683..4688,4689..4706,4707..4713)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4721..4737
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4726..4748
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4745..4761
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4745..4761
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4780..4800
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4782..4800
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    join(4857..4868,4869..5056)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     enhancer        5078..5457
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5459..5736
                     /label=chicken beta-actin promoter
     intron          5737..6753
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     6677..6699
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     6761..6780
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             6812..8239
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 gcggccgctc tagagtcgac gttaactaac ttaagctagc aacggtttcc ctctagcggg
       61 atcaattccg cccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg
      121 tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg
      181 gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg
      241 aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca
      301 aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct
      361 ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca
      421 cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa
      481 ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg
      541 cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg
      601 ggacgtggtt ttcctttgaa aaacacgata ataccatgat tgaacaagat ggattgcatg
      661 caggttcgcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
      721 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
      781 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
      841 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
      901 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
      961 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     1021 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     1081 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     1141 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
     1201 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     1261 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     1321 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     1381 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga actagaccta
     1441 agaagaagcg taaggtgtaa gtcgacggcc gccaccgcgg tggagctcga attaattcat
     1501 cgatgatgat ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat
     1561 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     1621 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
     1681 gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga
     1741 ttatgatcct ctagatcaga tctttcttgc ttttactttt acttccttaa tactcaagta
     1801 caattttaat ggagtacttt tttactttta ctcaagtaag attctagcca gatactttta
     1861 cttttaattg agtaaaattt tccctaagta cttgtacttt cacttgagta aaatttttga
     1921 gtacttttta cacctctgtc aagaaccata tgccggtacc caattcgccc tatagtgagt
     1981 cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     2041 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     2101 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc
     2161 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accaccacac
     2221 ccgccgcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     2281 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     2341 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     2401 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     2461 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     2521 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     2581 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     2641 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     2701 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     2761 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     2821 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     2881 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     2941 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     3001 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     3061 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     3121 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     3181 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     3241 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     3301 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     3361 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     3421 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     3481 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     3541 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     3601 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     3661 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     3721 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     3781 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     3841 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     3901 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     3961 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     4021 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     4081 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     4141 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     4201 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     4261 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     4321 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     4381 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     4441 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     4501 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     4561 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     4621 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     4681 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     4741 cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg
     4801 gaacaaaagc tggagctcgg atctgctggg cttgctgaag gtagggggtc aagaaccaga
     4861 ggtgtaaagt acttgagtaa ttttacttga ttactgtact taagtattat ttttggggat
     4921 ttttacttta cttgagtaca attaaaaatc aatactttta cttttactta attacatttt
     4981 tttagaaaaa aaagtacttt ttactcctta caattttatt tacagtcaaa aagtacttat
     5041 tttttggaga tcacttgggc cctctagatg catgctcgac attgattatt gactagttat
     5101 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     5161 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     5221 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     5281 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     5341 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     5401 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc
     5461 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt
     5521 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg gggggggggg
     5581 cgcgcgccgg gggggggggg gggggggggg gggsggggsg gggsgaggcg gagaggtgcg
     5641 gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg
     5701 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc
     5761 cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta
     5821 ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta gcgcttggtt
     5881 taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc
     5941 cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc
     6001 gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt
     6061 gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg
     6121 ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt
     6181 gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc ctccccgagt tgctgagcac
     6241 ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc
     6301 ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc cggggagggc
     6361 tcgggggagg ggcgcggcgg cccccggagc gccggcggct gtcgaggcgc ggcgagccgc
     6421 agccattgcc ttttatggta atcgtgcgag agggcgcagg gacttccttt gtcccaaatc
     6481 tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag
     6541 cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc
     6601 cgtccccttc tccctctcca gcctcggggc tgtccgcggg gggacggctg ccttcggggg
     6661 ggacggggca gggcggggtt cggcttctgg cgtgtgaccg gcggctctag agcctctgct
     6721 aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct ggttattgtg
     6781 ctgtctcatc attttggcaa aggaattcgc catggtgagc aagggcgagg aggtcatcaa
     6841 agagttcatg cgcttcaagg tgcgcatgga gggctccatg aacggccacg agttcgagat
     6901 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
     6961 caagggcggc cccctgccct tcgcctggga catcctgtcc ccccagttca tgtacggctc
     7021 caaggcgtac gtgaagcacc ccgccgacat ccccgattac aagaagctgt ccttccccga
     7081 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggtctggtga ccgtgaccca
     7141 ggactcctcc ctgcaggacg gcacgctgat ctacaaggtg aagatgcgcg gcaccaactt
     7201 cccccccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg
     7261 cctgtacccc cgcgacggcg tgctgaaggg cgagatccac caggccctga agctgaagga
     7321 cggcggccac tacctggtgg agttcaagac catctacatg gccaagaagc ccgtgcaact
     7381 gcccggctac tactacgtgg acaccaagct ggacatcacc tcccacaacg aggactacac
     7441 catcgtggaa cagtacgagc gctccgaggg ccgccaccac ctgttcctgg ggcatggcac
     7501 cggcagcacc ggcagcggca gctccggcac cgcctcctcc gaggacaaca acatggccgt
     7561 catcaaagag ttcatgcgct tcaaggtgcg catggagggc tccatgaacg gccacgagtt
     7621 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
     7681 ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc agttcatgta
     7741 cggctccaag gcgtacgtga agcaccccgc cgacatcccc gattacaaga agctgtcctt
     7801 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggtc tggtgaccgt
     7861 gacccaggac tcctccctgc aggacggcac gctgatctac aaggtgaaga tgcgcggcac
     7921 caacttcccc cccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctccac
     7981 cgagcgcctg tacccccgcg acggcgtgct gaagggcgag atccaccagg ccctgaagct
     8041 gaaggacggc ggccactacc tggtggagtt caagaccatc tacatggcca agaagcccgt
     8101 gcaactgccc ggctactact acgtggacac caagctggac atcacctccc acaacgagga
     8161 ctacaccatc gtggaacagt acgagcgctc cgagggccgc caccacctgt tcctgtacgg
     8221 catggacgag ctgtacaagt ctagaaagat gagcaaagat ggtaaaaaga agaaaaagaa
     8281 gtcaaagaca aagtgtgtaa ttatgtaa
//
