LOCUS       sequence-334493-        9996 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GoldenBraid Double Transcriptional Unit - 
            pEstradiol_Inducible_E-Luc_NtEUt+pNOS_Red-F_NOSt.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9996)
  AUTHORS   Gonzalez-Grandio E, Demirer GS, Ma W, Brady S, Landry MP
  TITLE     A Ratiometric Dual Color Luciferase Reporter for Fast 
            Characterization of Transcriptional Regulatory Elements in Plants.
  JOURNAL   ACS Synth Biol. 2021 Sep 14. doi: 10.1021/acssynbio.1c00248.
  PUBMED    34520169
REFERENCE   2  (bases 1 to 9996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2021 Sep 14. doi: 10.1021/acssynbio.1c00248."
COMMENT     Sequence Label: pGREAT26
FEATURES             Location/Qualifiers
     source          1..9996
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        205..251
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             280..531
                     /codon_start=1
                     /gene="lexA (truncated)"
                     /product="DNA-binding domain of E. coli LexA"
                     /label=LexA BD
                     /note="binds the 16-bp palindromic sequence 
                     5'-CTGTATATATATACAG-3'"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAA"
     CDS             547..780
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             790..1725
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             838..1731
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
     promoter        2455..2501
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     RBS             3991..3999
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     promoter        4728..4907
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     terminator      6612..6864
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(6899..6917)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    7106..7130
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(7221..7809)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7301..7320)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7980..8771)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      9415..9850
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    join(9977..9996,1..3)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
ORIGIN
        1 taacggatcc ggtctcagga gatagtttaa actgaaggcg ggaaacgaca atctgatcca
       61 agctcaagct aagcttgcat gcctgcagga tatcgtggat ccaagcttgc cacgtgccgc
      121 cacgtgccgc cacgtgccgc cacgtgcctc tagaggatcc atctccactg acgtaaggga
      181 tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca
      241 tttggagagg acacgctggg atccccaatt ccgggcggaa tgaaagcgtt aacggccagg
      301 caacaagagg tgtttgatct catccgtgat cacatcagcc agacaggtat gccgccgacg
      361 cgtgcggaaa tcgcgcagcg tttggggttc cgttccccaa acgcggctga agaacatctg
      421 aaggcgctgg cacgcaaagg cgttattgaa attgtttccg gcgcatcacg cgggattcgt
      481 ctgttgcagg aagaggaaga agggttgccg ctggtaggtc gtgtggctgc cggtgaaccg
      541 tcgagcgccc ccccgaccga tgtcagcctg ggggacgagc tccacttaga cggcgaggac
      601 gtggcgatgg cgcatgccga cgcgctagac gatttcgatc tggacatgtt gggggacggg
      661 gattccccgg gtccgggatt taccccccac gactccgccc cctacggcgc tctggatatg
      721 gccgacttcg agtttgagca gatgtttacc gatgcccttg gaattgacga gtacggtggg
      781 gatccgtctg ctggagacat gagagctgcc aacctttggc caagcccgct catgatcaaa
      841 cgctctaaga agaacagcct ggccttgtcc ctgacggccg accagatggt cagtgccttg
      901 ttggatgctg agccccccat actctattcc gagtatgatc ctaccagacc cttcagtgaa
      961 gcttcgatga tgggcttact gaccaacctg gcagacaggg agctggttca catgatcaac
     1021 tgggcgaaga gggtgccagg ctttgtggat ttgaccctcc atgatcaggt ccaccttcta
     1081 gaatgtgcct ggctagagat cctgatgatt ggtcttgtct ggcgctccat ggagcaccca
     1141 gggaagctac tgtttgctcc taacttgctc ttggacagga accagggaaa atgtgtagag
     1201 ggcatggtgg agatcttcga catgctgctg gctacatcat ctcggttccg catgatgaat
     1261 ctgcagggag aggagtttgt gtgcctcaaa tctattattt tgcttaattc tggagtgtac
     1321 acatttctgt ccagcaccct gaagtctctg gaagagaagg accatatcca ccgagtcctg
     1381 gacaagatca cagacacttt gatccacctg atggccaagg caggcctgac cctgcagcag
     1441 cagcaccagc ggctggccca gctcctcctc atcctctccc acatcaggca catgagtaac
     1501 aaaggcatgg agcatctgta cagcatgaag tgcaagaacg tggtgcccct ctatgacctg
     1561 ctgctggaga tgctggacgc ccaccgccta catgcgccca ctagccgtgg aggggcatcc
     1621 gtggaggaga cagaccaaag ccacttggcc actgcgggct ctacttcatc gcattccttg
     1681 caaaagtatt acatcacggg ggaggcagag ggtttccctg ccacagtctg agagctccct
     1741 ggcggaattc ccagagatgt tagctgaaat catcactaat cagataccaa aatattcaaa
     1801 tggaaatatc aaaaagcttc tgtttcatca aaaatgactc gacctaactg agtaagctag
     1861 cttgttcgag tattatggca ttgggaaaac tgtttttctt gtaccatttg ttgtgcttgt
     1921 aatttactgt gttttttatt cggttttcgc tatcgaactg tgaaatggaa atggatggag
     1981 aagagttaat gaatgatatg gtccttttgt tcattctcaa attaatatta tttgtttttt
     2041 ctcttatttg ttgtgtgttg aatttgaaat tataagagat atgcaaacat tttgttttga
     2101 gtaaaaatgt gtcaaatcgt ggcctctaat gaccgaagtt aatatgagga gtaaaacatc
     2161 ccaaacagct tgggctgcag gtcgaggcta aaaaactaat cgcattatca tcccctcgac
     2221 gtactgtaca tataaccact ggttttatat acagcagtac tgtacatata accactggtt
     2281 ttatatacag cagtcgacgt actgtacata taaccactgg ttttatatac agcagtactg
     2341 tacatataac cactggtttt atatacagca gtcgaggtaa gattagatat ggatatgtat
     2401 atggatatgt atatggtggt aatgccatgt aatatgctcg actctaggat cttcgcaaga
     2461 cccttcctct atataaggaa gttcatttca tttggagagg acacgctgaa gctagtcaat
     2521 ggagagagag aagaacgtgg tgtacggccc cgagcccaag caccctctgg gcaacttcac
     2581 cgccggcgag atgctgtaca acgctctgca caagcactcc cacatccccc aggccatcct
     2641 ggacgtgatg ggcaacgagt ccctttccta ccaggagttc ttcgacacta ctgtgaagct
     2701 gggccagagc ctccagaact gtggctacaa gatgaacgat gtcgtgtcga tctgtgcaga
     2761 gaacaacaag agattcttca tccccatcat ctccgcctgg tacatcggca tggtggtggc
     2821 ccctgtgaac gaggactata tcccagacga gctgtgtaaa gtgaccggca tctccaagcc
     2881 gatcctggtc tttaccacta ggaagatcct gcctaaggtt ttggaggtta aagacagaac
     2941 caactacata aagaggatca tcatactgga ctctgaagag aacctgctgg gctgcgagag
     3001 cctgcacaac ttcatgtcca ggtactccga caacaacctc caaacattca agcctctgca
     3061 ctacgaccct gtggaccagg tagccgccat cctgtgctcc tccggcacaa ccggcctgcc
     3121 taaaggcgtg atgcagaccc acaggaacat ctgtgtgaga ctcacacacg catctgaccc
     3181 cagagtgggt acacaactca tccccggcgt atccgtgctg gcctacctgc cattcttcca
     3241 cgccttcggc ttcagtatca acctgggcta tttcatggtg ggcctgagag tggtgatgct
     3301 ccgaaggttt aaccaggagg tgttcctgaa ggccatccag gactacgagg tgaggagcgt
     3361 gatcaacgtt ccctccacaa tcctgttcct gtccaagagc cctctggtgg acaagtacga
     3421 cctatccacc ctggcggagc tgtgctgtgg agccgctcct ctggcgaagg aggtggccga
     3481 gatcgccgtg aagaggctga acctgccagg gatacggtgt ggctacggtc taacagagtc
     3541 tacctccgcc aacatccata ctctgcacaa cgagttcaag tccggctccc tgggcaaggt
     3601 gacaccttac atggccgcca agatcatcga caggaacacc ggcgaggccc tgggtccaaa
     3661 ccaggtgggc gagctgtgca tctggggacc tatggtaaca aaaggctatg tgaacaaccc
     3721 acaggctact aaggaggcca tcgacgacga cggctggctg cactctggcg acttcggcta
     3781 ctacgacgag gacgagtatt tctacatcgt ggaccggtac aaggagctga tcaaatacaa
     3841 gggctatcag gtcgcccctg tggagctgga ggagatcctc cttcagcacc caggcatcag
     3901 ggacgtggcc gtcgtgggta tccctgacat cgaggccggc gagctgccag ccggcttcgt
     3961 ggtgaagcag cccggcgccc aactcaccgc taaggaggtg tacgacttcc tggcccagag
     4021 ggtgtctcac tccaagtacc tgaggggcgg cgtaaggttc gtggactcta tccccaggaa
     4081 cgtgacaggc aagattagtc gaaaagagct gagggaggcc ctgatggaga aggcttctaa
     4141 gctgtaagct taaagcagaa tgctgagcta aaagaaaggc tttttccatt ttcgagagac
     4201 aatgagaaaa gaagaagaag aagaagaaga agaagaagaa gaagaagaaa agagtaaata
     4261 ataaagcccc acaggaggcg aagttcttgt agctccatgt tatctaagtt attgatattg
     4321 tttgccctat attttatttc tgtcattgtg tatgttttgt tcagtttcga tctccttgca
     4381 aaatgcagag attatgagat gaataaacta agttatatta ttatacgtgt taatattctc
     4441 ctcctctctc tagctagcct tttgttttct ctttttctta tttgattttc tttaaatcaa
     4501 tccattttag gagagggcca gggagtgatc cagcaaaaca tgaagattag aagaaacttc
     4561 cctctttttt ttcctgaaaa caatttaacg tcgagattta tctctttttg taatggaatc
     4621 atttctacag ttatgaccgc tgtcaggaga tacatgagaa ttaagggagt cacggtatga
     4681 cccccgccga tgacgcggga caagccgttt tacgtttgga actgacagaa ccgcaacgtt
     4741 gaaggagcca ctgagccgcg ggtttctgga gtttaatgag ctaagcacat acgtcagaaa
     4801 ccattattgc gcgttcaaaa gtcgcctaag gtcactatca gctagcaaat atttcttgtc
     4861 aaaaatgctc cactgacgtt ccataaattc ccctcggtat ccaattagag tctcatattc
     4921 actctcaact cgatcgaggc aatggagaac atggagaacg acgagaacat tgtggtggga
     4981 cccaagccgt tctacccgat cgaggagggt tccgctggca cccagctgcg caagtacatg
     5041 gagcgctacg ctaagctggg tgctatcgcc ttcaccaacg ccgtgaccgg tgtggattac
     5101 tcctacgccg agtacctgga gaagtcctgc tgcctcggaa aggccctgca gaactacggt
     5161 ctggtcgtgg atggccgcat tgccctctgc tcggagaact gcgaggagtt cttcattccc
     5221 gtgatcgctg gtctgttcat cggagtcggc gttgccccaa ccaacgagat ctacaccctg
     5281 cgcgagctgg tccacagcct gggtatcagc aagccgacca tcgttttctc gagcaagaag
     5341 ggcctcgaca aggttatcac cgtgcaaaaa accgtcacca ccatcaagac catcgttatc
     5401 ctggattcca aggttgacta ccgcggttac cagtgcctcg acaccttcat caagcgtaac
     5461 accccacccg gcttccaagc ctcctccttc aaaaccgtgg aggttgatcg caaggagcag
     5521 gtggccctga tcatgaactc ctccggctcc accggtctgc caaagggcgt gcagctgacc
     5581 cacgagaaca ccgtcacccg cttctcgcat gcccgtgatc ccatctacgg caaccaggtg
     5641 tccccaggca ccgccgtcct gaccgtcgtg cccctccacc acggcttcgg catgttcacc
     5701 actctcggct acttgatctg cggcttccgt gtggtgatgc tgactaagtt cgacgaggag
     5761 actttcctca agaccctgca agactacaag tgcacttacg tgatcctggt gccgaccctg
     5821 ttcgccatcc tgaacaagag cgagctgttg aacaaatacg acctgtcgaa cctggtggag
     5881 atcgcttccg gcggcgcccc actgtcgaag gaagttggcg aggccgttgc tcgccgcttc
     5941 aacctgcccg gcgtccgtca gggttacggc ctgaccgaga ctacctcggc tatcatcatc
     6001 acccccgagg gcgacgataa gcccggcgct tccggcaagg ttgttccact cttcaaagcc
     6061 aaggtgatcg acctggacac caagaagtcc ctgggtccca accgtcgcgg agaagtgtgc
     6121 gtcaagggcc ccatgctgat gaagggctac gtcaacaacc cagaggccac caaggagttg
     6181 atcgacgagg agggctggct gcacactggt gacatcggtt actacgacga agagaagcac
     6241 ttcttcatcg ttgaccgcct gaagtccttg atcaagtaca agggctacca ggttcccccc
     6301 gccgagctcg agagcgtcct gctgcagcac ccctccattt tcgatgccgg agtggccggt
     6361 gtccccgatc ccgtggctgg cgagctgcca ggtgccgtcg tcgtgctgga gtccggcaag
     6421 aacatgaccg agaaggaagt gatggattac gtggcctcgc aagtgtccaa cgccaagcgc
     6481 ctgcgcggcg gcgtgcgctt cgtggatgag gtgcccaagg gcctcaccgg aaagatcgac
     6541 ggtcgcgcta tccgcgagat cctcaagaag cccgtggcca agatgtgagc ttggaatgga
     6601 tcttcgatcc cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg
     6661 ccggtcttgc gacgattatc atataatttc tgttgaatta cgttaagcat gtaataatta
     6721 acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat
     6781 acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg
     6841 cggtgtcatc tatgttacta gatcgggaat tgccaagcta attcttgaag atgaaagggc
     6901 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     6961 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     7021 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atgggaccga
     7081 ctcgcgctgt catgagaccg gatcctgaca ggatatattg gcgggtaaac taagtcgctg
     7141 tatgtgtttg tttgagatct catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     7201 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     7261 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     7321 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     7381 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     7441 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     7501 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     7561 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     7621 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     7681 gctctgctga agccagttac cttcggaaga agagttggta gctcttgatc cggcaaacaa
     7741 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     7801 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     7861 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     7921 aattaaaaat gaagttttaa atcaatctaa agtatatatg tgtaacattg gtctagtgat
     7981 tatttgccga ctaccttggt gatctcgcct ttcacgtagt ggacaaattc ttccaactga
     8041 tctgcgcgcg aggccaagcg atcttcttct tgtccaagat aagcctgtct agcttcaagt
     8101 atgacgggct gatactgggc cggcaggcgc tccattgccc agtcggcagc gacatccttc
     8161 ggcgcgattt tgccggttac tgcgctgtac caaatgcggg acaacgtaag cactacattt
     8221 cgctcatcac cagcccagtc gggcggcgag ttccatagcg ttaaggtttc atttagcgcc
     8281 tcaaatagat cctgttcagg aaccggatca aagagttcct ccgccgctgg acctaccaag
     8341 gcaacgctat gttctcttgc ttttgtcagc aagatagcca gatcaatgtc gatcgtggct
     8401 ggctcgaaga tacctgcaag aatgtcattg cgctgccatt ctccaaattg cagttcgcgc
     8461 ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa caatggtgac ttctacagcg
     8521 cggagaatct cgctctctcc aggggaagcc gaagtttcca aaaggtcgtt gatcaaagct
     8581 cgccgcgttg tttcatcaag ccttacggtc accgtaacca gcaaatcaat atcactgtgt
     8641 ggcttcaggc cgccatccac tgcggagccg tacaaatgta cggccagcaa cgtcggttcg
     8701 agatggcgct cgatgacgcc aactacctct gatagttgag tcgatacttc ggcgatcacc
     8761 gcttccctca taatgtttaa ctttgtttta gggcgactgc cctgctgcgt aacatcgttg
     8821 ctgctccata acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga
     8881 ggcatagact gtaccccaaa aaaacagtca taacaagcca tgaaaaccgc cactgcgccg
     8941 ttaccaccgc tgcgttcggt caaggttctg gaccagttgc gtgagcgcat acgctacttg
     9001 cattacagct tacgaaccga acaggcttat gtccactggg ttcgtgcctt catccgtttc
     9061 cacggtgtgc gtcacccggc aaccttgggt agcagcgaag tcgaggcatt tctgtcctgg
     9121 ctggaacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgatgata
     9181 tatttttatc ttgtgcaatg taacatcaga gattttgaga cacaacgtgg ctttgttgaa
     9241 taaatcgaac ttttgctgag ttgaaggatc agatcacgca tcttcccgac aacgcagacc
     9301 gttccgtggc aaagcaaaag ttcaaaatca ccaactggtc cacctacaac aaagctctca
     9361 tcaaccgtgg ctccctcact ttctggctgg atgatggggc gattcaggcg atccccatcc
     9421 aacagcccgc cgtcgagcgg gcttttttat ccccggaagc ctgtggatag agggtagtta
     9481 tccacgtgaa accgctaatg ccccgcaaag ccttgattca cggggctttc cggcccgctc
     9541 caaaaactat ccacgtgaaa tcgctaatca gggtacgtga aatcgctaat cggagtacgt
     9601 gaaatcgcta ataaggtcac gtgaaatcgc taatcaaaaa ggcacgtgag aacgctaata
     9661 gccctttcag atcaacagct tgcaaacacc cctcgctccg gcaagtagtt acagcaagta
     9721 gtatgttcaa ttagcttttc aattatgaat atatatatca attattggtc gcccttggct
     9781 tgtggacaat gcgctacgcg caccggctcc gcccgtggac aaccgcaagc ggttgcccac
     9841 cgtcgagcgc ctttgcccac aacccggcgg ccggccgcaa cagatcgttt tataaatttt
     9901 tttttttgaa aaagaaaaag cccgaaaggc ggcaacctct cgggcttctg gatttccgat
     9961 ccccggaatt agatcttggc aggatatatt gtggtg
//
