LOCUS       sequence-334494-       11813 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express Ikkbeta (S177E, S181E) in 
            mammalian cells under control of EF1a promoter. Barcoded for 
            transcript detection in single cell RNA-seq.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11813)
  AUTHORS   Parekh U, McDonald D, Dailamy A, Wu Y, Cordes T, Zhang K, Tipps A, 
            Metallo C, Mali P
  TITLE     Charting oncogenicity of genes and variants across lineages via 
            multiplexed screens in teratomas.
  JOURNAL   iScience. 2021 Sep 20;24(10):103149. doi: 
            10.1016/j.isci.2021.103149. eCollection 2021 Oct 22.
  PUBMED    34646987
REFERENCE   2  (bases 1 to 11813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2021-09-20"; volume: "24(10):103149. doi"; pages: " 
            10.1016/j.isci.2021.103149. eCollection 2021 Oct 22"
COMMENT     Sequence Label: LV_EF1a_FLAG-Ikkbeta(S177E,S181E)-P2A-Hygro_Ba...
FEATURES             Location/Qualifiers
     source          1..11813
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2693..3871
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2924..3862
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3819..3839
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     3888..3907
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3888..3906
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      3964..3973
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3973..3996
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             6286..6342
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6343..7368
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6343..7368
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(7560..7593)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             7618..7798
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7824..7841)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7830..8054
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8100..8528
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8187..8206)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8397..8418
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8537..8557)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8542..8871
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8722..8857
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8784..8803
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8919..8966
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8985..9359
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9489..9610
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9526..9545)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9580..9599
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9659..9675)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9659..9675)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9672..9694)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9683..9699
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9707..9713,9714..9731,9732..9737))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9752..9773
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9890..9907)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10061..10649)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10141..10160)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10820..11611,11612..11680))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11443..11462
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11681..11785)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta atcgataagc tttgcaaaga tggataaagt tttaaacaga
     2641 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg
     2701 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
     2761 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
     2821 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
     2881 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg
     2941 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac
     3001 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc
     3061 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg
     3121 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa
     3181 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag
     3241 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg
     3301 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc
     3361 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc
     3421 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg
     3481 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg
     3541 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc
     3601 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc
     3661 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc
     3721 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt
     3781 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca
     3841 aagttttttt cttccatttc aggtgtcgtg aggaattagc ttggtactaa tacgactcac
     3901 tatagggaga cccaagctgg ctaggtaagc ttgatatcga attcctgcag cccgggggat
     3961 cccgccacca tggactacaa agacgatgac gacaagcttg cggccgcaat gagctggtca
     4021 ccttccctga caacgcagac atgtggggcc tgggaaatga aagagcgcct tgggacaggg
     4081 ggatttggaa atgtcatccg atggcacaat caggaaacag gtgagcagat tgccatcaag
     4141 cagtgccggc aggagctcag cccccggaac cgagagcggt ggtgcctgga gatccagatc
     4201 atgagaaggc tgacccaccc caatgtggtg gctgcccgag atgtccctga ggggatgcag
     4261 aacttggcgc ccaatgacct gcccctgctg gccatggagt actgccaagg aggagatctc
     4321 cggaagtacc tgaaccagtt tgagaactgc tgtggtctgc gggaaggtgc catcctcacc
     4381 ttgctgagtg acattgcctc tgcgcttaga taccttcatg aaaacagaat catccatcgg
     4441 gatctaaagc cagaaaacat cgtcctgcag caaggagaac agaggttaat acacaaaatt
     4501 attgacctag gatatgccaa ggagctggat cagggcgaac tttgcacaga attcgtgggg
     4561 accctgcagt acctggcccc agagctactg gagcagcaga agtacacagt gaccgtcgac
     4621 tactggagct tcggcaccct ggcctttgag tgcatcacgg gcttccggcc cttcctcccc
     4681 aactggcagc ccgtgcagtg gcattcaaaa gtgcggcaga agagtgaggt ggacattgtt
     4741 gttagcgaag acttgaatgg aacggtgaag ttttcaagct ctttacccta ccccaataat
     4801 cttaacagtg tcctggctga gcgactggag aagtggctgc aactgatgct gatgtggcac
     4861 ccccgacaga ggggcacgga tcccacgtat gggcccaatg gctgcttcaa ggccctggat
     4921 gacatcttaa acttaaagtt ggttcatatc ttgaacatgg tcacgggcac catccacacc
     4981 taccctgtga cagaggatga gagtctgcag agcttgaagg ccagaatcca acaggacacg
     5041 ggcatcccag aggaggacca ggagctgctg caggaagcgg gcctggcgtt gatccccgat
     5101 aagcctgcca ctcagtgtat ttcagacggc aagttaaatg agggccacac attggacatg
     5161 gatcttgttt ttctctttga caacagtaaa atcacctatg agactcagat ctccccacgg
     5221 ccccaacctg aaagtgtcag ctgtatcctt caagagccca agaggaatct cgccttcttc
     5281 cagctgagga aggtgtgggg ccaggtctgg cacagcatcc agaccctgaa ggaagattgc
     5341 aaccggctgc agcagggaca gcgagccgcc atgatgaatc tcctccgaaa caacagctgc
     5401 ctctccaaaa tgaagaattc catggcttcc atgtctcagc agctcaaggc caagttggat
     5461 ttcttcaaaa ccagcatcca gattgacctg gagaagtaca gcgagcaaac cgagtttggg
     5521 atcacatcag ataaactgct gctggcctgg agggaaatgg agcaggctgt ggagctctgt
     5581 gggcgggaga acgaagtgaa actcctggta gaacggatga tggctctgca gaccgacatt
     5641 gtggacttac agaggagccc catgggccgg aagcaggggg gaacgctgga cgacctagag
     5701 gagcaagcaa gggagctgta caggagacta agggaaaaac ctcgagacca gcgaactgag
     5761 ggtgacagtc aggaaatggt acggctgctg cttcaggcaa ttcagagctt cgagaagaaa
     5821 gtgcgagtga tctatacgca gctcagtaaa actgtggttt gcaagcagaa ggcgctggaa
     5881 ctgttgccca aggtggaaga ggtggtgagc ttaatgaatg aggatgagaa gactgttgtc
     5941 cggctgcagg agaagcggca gaaggagctc tggaatctcc tgaagattgc ttgtagcaag
     6001 gtccgtggtc ctgtcagtgg aagcccggat agcatgaatg cctctcgact tagccagcct
     6061 gggcagctga tgtctcagcc ctccacggcc tccaacagct tacctgagcc agccaagaag
     6121 agtgaagaac tggtggctga agcacataac ctctgcaccc tgctagaaaa tgccatacag
     6181 gacactgtga gggaacaaga ccagagtttc acggccctag actggagctg gttacagacg
     6241 gaagaagaag agcacagctg cctggagcag gcctcaggat ccggcgcaac aaacttctct
     6301 ctgctgaaac aagccggaga tgtcgaagag aatcctggac cgatgaaaaa gcctgaactc
     6361 accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg
     6421 cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat
     6481 gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac
     6541 tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggaatt cagcgagagc
     6601 ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc
     6661 gaactgcccg ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat
     6721 cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca
     6781 tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg
     6841 gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag
     6901 gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg
     6961 gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa
     7021 tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg
     7081 cgctacttcg agcggaggca tccggagctt gcaggatcgc cgcggctccg ggcgtatatg
     7141 ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca
     7201 gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt
     7261 acacaaatcg cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc
     7321 gatagtggaa accgacgccc cagcactcgt ccgagggcaa aggaataagt tagaactatt
     7381 tcctggctgt tacgcgctag tccatttagt atcggcctac tgacgggctt gttaaacagt
     7441 gaaccgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc
     7501 gaacaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatatca
     7561 taacttcgta tagcatacat tatacgaagt tatcggctag ctggtccgga ctgtactggg
     7621 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     7681 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     7741 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagg
     7801 gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     7861 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     7921 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     7981 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     8041 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
     8101 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     8161 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     8221 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     8281 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     8341 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     8401 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     8461 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     8521 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     8581 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     8641 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     8701 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     8761 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
     8821 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
     8881 ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa tcatcggcat
     8941 agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc aagttgacca
     9001 gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc
     9061 ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg
     9121 tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg
     9181 tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact
     9241 tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt
     9301 tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgac
     9361 acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     9421 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     9481 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     9541 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     9601 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
     9661 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     9721 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     9781 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     9841 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     9901 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     9961 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
    10021 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
    10081 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
    10141 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
    10201 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
    10261 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
    10321 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
    10381 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
    10441 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
    10501 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
    10561 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
    10621 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
    10681 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
    10741 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
    10801 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
    10861 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
    10921 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
    10981 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
    11041 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
    11101 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
    11161 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
    11221 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
    11281 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
    11341 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
    11401 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
    11461 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
    11521 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
    11581 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
    11641 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
    11701 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
    11761 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gac
//
