LOCUS       sequence-334499-       11102 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For generating the conditional transgenic mice expressing BRET-FRET 
            hybrid biosensor of ERK..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11102)
  AUTHORS   Ichise H, Tsukamoto S, Hirashima T, Konishi Y, Oki C, Tsukiji S, 
            Iwano S, Miyawaki A, Sumiyama K, Terai K, Matsuda M
  TITLE     Functional visualization of NK Cell-mediated killing of metastatic 
            single tumor cells.
  JOURNAL   Elife. 2022 Feb 3;11. pii: 76269. doi: 10.7554/eLife.76269.
  PUBMED    35113018
REFERENCE   2  (bases 1 to 11102)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-02-3"; volume: "11. pii: 76269. doi"; pages: " 
            10.7554/eLife.76269"
COMMENT     Sequence Label: pT2ADW-lox-mCherry-hyBRET-ERK-NLS
FEATURES             Location/Qualifiers
     source          1..11102
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..34)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(47..49,50..52,53..760)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLLCTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLL
                     EFLTAAGITEGMNELY"
     CDS             935..1282
                     /codon_start=1
                     /product="long flexible linker consisting of SAGG repeats 
                     (Komatsu et al., 2011)"
                     /label=EV linker
                     /translation="SAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGS
                     AGGSAGGXGSAGGSAGGSTSAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGG
                     SAGGSAGGSAGG"
     CDS             2975..2995
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    3010..3598
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3063..3083)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3481..3492)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3663..3797
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3687..3706)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3741..3760
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(3919..4081,4082..4093)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(4123..4142)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4124..4142)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4152..4169)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4152..4168)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4161..4183)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4310..4764
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4396..4415)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4606..4627
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4790..4894
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4895..4963,4964..5755)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5113..5132)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5926..6514
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6415..6434
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6668..6685
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6802..6823
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6838..6843,6844..6861,6862..6868)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     6900..6916
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6900..6916
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6935..6955
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6937..6955
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    join(7012..7023,7024..7211)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     enhancer        8589..8968
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          9248..10264
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     10188..10210
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     10272..10291
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(10318..10351)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(10500..10518)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     10768..10787
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             11069..11095
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
ORIGIN
        1 ataacttcgt atagcataca ttatacgaag ttataccgaa ttcggcatgg tgagcaaggg
       61 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
      121 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
      181 gaagcttcta tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
      241 gggctacggc ctgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt
      301 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
      361 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
      421 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
      481 ctacaacagc cacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa
      541 cttcaagatc cgccacaaca tcgaggacgg cggcgtgcag ctcgccgacc actaccagca
      601 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca
      661 gtccgccctg ttcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcct
      721 gaccgccgcc gggatcactg agggcatgaa cgagctgtac ctcgagatgg cggacgagga
      781 gaagctgccg cccggctggg agaagcgcat gagccgcagc tcaggccgag tgtactactt
      841 caaccacatc actaacgcca gccagtggga gcggcccagc ggcaacagca gcagtggtgg
      901 caaaaacggg cagggggagc ctgccagggg taccagtgct ggtggtagtg ctggtggtag
      961 tgctggtggt agtgctggtg gtagtgctgg tggttccggc agtgctggtg gtagtgctgg
     1021 tggtagtacc agtgctggtg gtagtgctgg tggtagtgct ggtggtagtg ctggtggtag
     1081 tgctggtggt tscggcagtg ctggtggtag tgctggtggt agtaccagtg ctggtggtag
     1141 tgctggtggt agtgctggtg gtagtgctgg tggtagtgct ggtggttccg gcagtgctgg
     1201 tggtagtgct ggtggtagta ccagtgctgg tggtagtgct ggtggtagtg ctggtggtag
     1261 tgctggtggt agtgctggtg gttccggacc agatgtccct agaactccag tggataaagc
     1321 aaagctgtca ttccaatttc cgggcggccg catggtgagc aagggcgagg agctgttcac
     1381 cggggtggtg cccatcctgg tcgagctgga cggcgacgtc aacggccaca agttcagcgt
     1441 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     1501 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgtcct ggggcgtgca
     1561 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     1621 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     1681 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     1741 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactact tcagcgggaa
     1801 cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca
     1861 caacatcgag gacggcggcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     1921 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccttaagcaa
     1981 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcttgaccg ccgccggggg
     2041 taccaaggtg tacgaccccg agcagaggaa gaggatgatc accggccccc agtggtgggc
     2101 caggtgcaag cagatgaacg tgctggacag cttcatcaac tactacgaca gcgagaagca
     2161 cgccgagaac gccgtgatct tcctgcacgg caacgccact agcagctacc tgtggaggca
     2221 cgtggtgccc cacatcgagc ccgtggccag gtgcatcatc cccgatctga tcggcatggg
     2281 caagagcggc aagagcggca acggcagcta caggctgctg gaccactaca agtacctgac
     2341 cgcctggttc gagcttctga acctgcccaa gaagatcatc ttcgtgggcc acgactgggg
     2401 cgccgccctg gccttccact acgcctacga gcaccaggac aggatcaagg ccatcgtgca
     2461 catggagagc gtggtggacg tgatcgagag ctgggacgag tggccagaca tcgaggagga
     2521 catcgccctg atcaagagcg aggagggcga gaagatggtg ctggagaaca acttcttcgt
     2581 ggagaccgtg ctgcccagca agatcatgag aaagctggag cccgaggagt tcgccgccta
     2641 cctggagccc ttcaaggaga agggcgaggt gagaagaccc accctgagct ggcccagaga
     2701 gatccccctg gtgaagggcg gcaagcccga cgtggtgcag atcgtgagaa actacaacgc
     2761 ctacctgaga gccagcgacg acctgcccaa gctgttcatc gagggcgacc ccggcttctt
     2821 cagcaacgcc atcgtggagg gcgccaagaa gttccccaac accgagttcg tgaaggtgaa
     2881 gggcctgcac ttcctccagg aggacgcccc cgacgagatg ggcaagtaca tcaagagctt
     2941 cgtggagaga gtgctgaaga acgagcagtc tagacctaag aagaagcgta aggtgtaagg
     3001 atcctcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     3061 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     3121 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     3181 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     3241 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     3301 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     3361 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     3421 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     3481 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     3541 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat
     3601 ctcgacaaag gatccaatgg aggccgccac cgcggtggag ctcgaattaa ttcatcgatg
     3661 atgatccaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
     3721 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     3781 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
     3841 aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg
     3901 atcctctaga tcagatcttt cttgctttta cttttacttc cttaatactc aagtacaatt
     3961 ttaatggagt acttttttac ttttactcaa gtaagattct agccagatac ttttactttt
     4021 aattgagtaa aattttccct aagtacttgt actttcactt gagtaaaatt tttgagtact
     4081 ttttacacct ctgtcaagaa ccatatgccg gtacccaatt cgccctatag tgagtcgtat
     4141 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     4201 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     4261 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     4321 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccmc cacacccgcc
     4381 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     4441 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     4501 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     4561 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     4621 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     4681 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     4741 acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     4801 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     4861 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     4921 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     4981 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     5041 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     5101 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     5161 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     5221 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     5281 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     5341 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     5401 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     5461 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     5521 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     5581 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     5641 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     5701 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     5761 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     5821 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     5881 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     5941 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     6001 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     6061 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     6121 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     6181 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     6241 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     6301 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     6361 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     6421 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     6481 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     6541 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     6601 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     6661 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     6721 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     6781 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     6841 acactttatg cttccggctc gtatgttgtg tggaattgtg atcggataac aatttcacac
     6901 aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca
     6961 aaagctggag ctcggatctg ctgggcttgc tgaaggtagg gggtcaagaa ccagaggtgt
     7021 aaagtacttg agtaatttta cttgattact gtacttaagt attatttttg gggattttta
     7081 ctttacttga gtacaattaa aaatcaatac ttttactttt acttaattac atttttttag
     7141 aaaaaaaagt actttttact ccttacaatt ttatttacag tcaaaaagta cttatttttt
     7201 ggagatcact tgggccctct agatgcatgc cggctggccc aggccatcgg cattccggag
     7261 cccagggtcc agatttggtt tcagaatgag aggtcacgcc agctgaggca gcaccggcgg
     7321 gaatctcggc cctggcccgg gagacgcggc ccgccagaag gccggcgaaa gcggaccgcc
     7381 gtcaccggat cccagaccgc cctgctcctc cgagcctttg agaaggatcg ctttccaggc
     7441 atcgccgccc gggaggagct ggccagagag acgggcctcc cggagtccag gattcagatc
     7501 tggtttcaga atcgaagggc caggcacccg ggacagggtg gcagggcgcc cgcgcaggca
     7561 ggcggcctgt gcagcgcggc ccccggcggg ggtcaccctg ctccctcgtg ggtcgccttc
     7621 gcccacaccg gcgcgtgggg aacggggctt cccgcacccc acgtgccctg cgcgcctggg
     7681 gctctcccac agggggcttt cgtgagccag gcagcgaggg ccgcccccgc gctgcagccc
     7741 agccaggccg cgccggcaga ggggatctcc caacctgccc cggcgcgcgg ggatttcgcc
     7801 tacgccgccc cggctcctcc ggacggggcg ctctcccacc ctcaggctcc tcgctggcct
     7861 ccgcacccgg gcaaaagccg ggaggaccgg gacccgcagc gcgacggcct gccgggcccc
     7921 tgcgcggtgg cacagcctgg gcccgctcaa gcggggccgc agggccaagg ggtgcttgcg
     7981 ccacccacgt cccaggggag tccgtggtgg ggctggggcc ggggtcccca ggtcgccggg
     8041 gcggcgtggg aaccccaagc cggggcagct ccacctcccc agcccgcgcc cccggacgcc
     8101 tccgcctccg cgcggcaggg gcagatgcaa ggcatcccgg cgccctccca ggcgctccag
     8161 gagccggcgc cctggtctgc actcccctgc ggcctgctgc tggatgagct cctggcgagc
     8221 ccggagtttc tgcagcaggc gcaacctctc ctagaaacgg aggccccggg ggagctggag
     8281 gcctcggaag aggccgcctc gctggaagca cccctcagcg aggaagaata ccgggctctg
     8341 ctggaggagc tttaggacgc ggggttggga cggggtcggg tggttcgggg cagggccgtg
     8401 gcctctcttt cgcggggaac acctggctgg ctacggaggg gcgtgtctcc gccccgcccc
     8461 ctccaccggg ctgaccggcc tgggattcct gccttctagg tctaggcccg gtgagagact
     8521 ccacaccgcg gagaactgcc attctttcct gggcatcccg gggatcccag agccggccca
     8581 gcatgctcga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
     8641 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
     8701 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
     8761 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
     8821 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
     8881 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
     8941 ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct
     9001 ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt aattattttg
     9061 tgcagcgatg ggggcggggg gggggggggg gcgcgcgccg gggggggggg gggggggsgg
     9121 ggggsggggs ggggsgrggs ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg
     9181 aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg
     9241 gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg
     9301 ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc
     9361 ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct
     9421 gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg
     9481 tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg
     9541 tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc
     9601 ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg
     9661 gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc
     9721 cctgcacccc cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac
     9781 ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg
     9841 cggggcgggg ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag
     9901 cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga
     9961 gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg
    10021 ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg
    10081 cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg
    10141 ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg
    10201 gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct
    10261 acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattata
    10321 acttcgtata gcatacatta tacgaagtta taccatggtg agcaagggcg aggaggataa
    10381 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
    10441 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
    10501 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
    10561 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa
    10621 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
    10681 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct
    10741 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
    10801 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag
    10861 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa
    10921 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca
    10981 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccactct
    11041 cggcatggac gagctgtcta gactgcagct gcctcctctg gaacgcctga ctctggatta
    11101 ag
//
