LOCUS       sequence-334506-        8142 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GoldenBraid Double Transcriptional Unit - 
            pAtUBQ10_E-Luc_NOSt+pNOS_Red-F_NOSt.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8142)
  AUTHORS   Gonzalez-Grandio E, Demirer GS, Ma W, Brady S, Landry MP
  TITLE     A Ratiometric Dual Color Luciferase Reporter for Fast 
            Characterization of Transcriptional Regulatory Elements in Plants.
  JOURNAL   ACS Synth Biol. 2021 Sep 14. doi: 10.1021/acssynbio.1c00248.
  PUBMED    34520169
REFERENCE   2  (bases 1 to 8142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2021 Sep 14. doi: 10.1021/acssynbio.1c00248."
COMMENT     Sequence Label: pGREAT3
FEATURES             Location/Qualifiers
     source          1..8142
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             2131..2139
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     terminator      2311..2563
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        2874..3053
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     terminator      4758..5010
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    5252..5276
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(5367..5955)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5447..5466)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6126..6917)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7561..7996
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    join(8123..8142,1..3)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
ORIGIN
        1 taacggatcc ggtctcagga ggtcgacgag tcagtaataa acggcgtcaa agtggttgca
       61 gccggcacac acgagtcgtg tttatcaact caaagcacaa atacttttcc tcaacctaaa
      121 aataaggcaa ttagccaaaa acaactttgc gtgtaaacaa cgctcaatac acgtgtcatt
      181 ttattattag ctattgcttc accgccttag ctttctcgtg acctagtcgt cctcgtcttt
      241 tcttcttctt cttctataaa acaataccca aagagctctt cttcttcaca attcagattt
      301 caatttctca aaatcttaaa aactttctct caattctctc taccgtgatc aaggtaaatt
      361 tctgtgttcc ttattctctc aaaatcttcg attttgtttt cgttcgatcc caatttcgta
      421 tatgttcttt ggtttagatt ctgttaatct tagatcgaag atgattttct gggtttgatc
      481 gttagatatc atcttaattc tcgattaggg tttcatagat atcatccgat ttgttcaaat
      541 aatttgagtt ttgtcgaata attactcttc gatttgtgat ttctatctag atctggtgtt
      601 agtttctagt ttgtgcgatc gaatttgtcg attaatctga gtttttctga ttaacagaat
      661 ggagagagag aagaacgtgg tgtacggccc cgagcccaag caccctctgg gcaacttcac
      721 cgccggcgag atgctgtaca acgctctgca caagcactcc cacatccccc aggccatcct
      781 ggacgtgatg ggcaacgagt ccctttccta ccaggagttc ttcgacacta ctgtgaagct
      841 gggccagagc ctccagaact gtggctacaa gatgaacgat gtcgtgtcga tctgtgcaga
      901 gaacaacaag agattcttca tccccatcat ctccgcctgg tacatcggca tggtggtggc
      961 ccctgtgaac gaggactata tcccagacga gctgtgtaaa gtgaccggca tctccaagcc
     1021 gatcctggtc tttaccacta ggaagatcct gcctaaggtt ttggaggtta aagacagaac
     1081 caactacata aagaggatca tcatactgga ctctgaagag aacctgctgg gctgcgagag
     1141 cctgcacaac ttcatgtcca ggtactccga caacaacctc caaacattca agcctctgca
     1201 ctacgaccct gtggaccagg tagccgccat cctgtgctcc tccggcacaa ccggcctgcc
     1261 taaaggcgtg atgcagaccc acaggaacat ctgtgtgaga ctcacacacg catctgaccc
     1321 cagagtgggt acacaactca tccccggcgt atccgtgctg gcctacctgc cattcttcca
     1381 cgccttcggc ttcagtatca acctgggcta tttcatggtg ggcctgagag tggtgatgct
     1441 ccgaaggttt aaccaggagg tgttcctgaa ggccatccag gactacgagg tgaggagcgt
     1501 gatcaacgtt ccctccacaa tcctgttcct gtccaagagc cctctggtgg acaagtacga
     1561 cctatccacc ctggcggagc tgtgctgtgg agccgctcct ctggcgaagg aggtggccga
     1621 gatcgccgtg aagaggctga acctgccagg gatacggtgt ggctacggtc taacagagtc
     1681 tacctccgcc aacatccata ctctgcacaa cgagttcaag tccggctccc tgggcaaggt
     1741 gacaccttac atggccgcca agatcatcga caggaacacc ggcgaggccc tgggtccaaa
     1801 ccaggtgggc gagctgtgca tctggggacc tatggtaaca aaaggctatg tgaacaaccc
     1861 acaggctact aaggaggcca tcgacgacga cggctggctg cactctggcg acttcggcta
     1921 ctacgacgag gacgagtatt tctacatcgt ggaccggtac aaggagctga tcaaatacaa
     1981 gggctatcag gtcgcccctg tggagctgga ggagatcctc cttcagcacc caggcatcag
     2041 ggacgtggcc gtcgtgggta tccctgacat cgaggccggc gagctgccag ccggcttcgt
     2101 ggtgaagcag cccggcgccc aactcaccgc taaggaggtg tacgacttcc tggcccagag
     2161 ggtgtctcac tccaagtacc tgaggggcgg cgtaaggttc gtggactcta tccccaggaa
     2221 cgtgacaggc aagattagtc gaaaagagct gagggaggcc ctgatggaga aggcttctaa
     2281 gctgtaagct tggaatggat cttcgatccc gatcgttcaa acatttggca ataaagtttc
     2341 ttaagattga atcctgttgc cggtcttgcg acgattatca tataatttct gttgaattac
     2401 gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg
     2461 attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac
     2521 taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgggaatt gccaagctaa
     2581 ttcttgaaga tgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     2641 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     2701 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     2761 gcttcaataa tgggaccgac tcgcgctgtc aggagataca tgagaattaa gggagtcacg
     2821 gtatgacccc cgccgatgac gcgggacaag ccgttttacg tttggaactg acagaaccgc
     2881 aacgttgaag gagccactga gccgcgggtt tctggagttt aatgagctaa gcacatacgt
     2941 cagaaaccat tattgcgcgt tcaaaagtcg cctaaggtca ctatcagcta gcaaatattt
     3001 cttgtcaaaa atgctccact gacgttccat aaattcccct cggtatccaa ttagagtctc
     3061 atattcactc tcaactcgat cgaggcaatg gagaacatgg agaacgacga gaacattgtg
     3121 gtgggaccca agccgttcta cccgatcgag gagggttccg ctggcaccca gctgcgcaag
     3181 tacatggagc gctacgctaa gctgggtgct atcgccttca ccaacgccgt gaccggtgtg
     3241 gattactcct acgccgagta cctggagaag tcctgctgcc tcggaaaggc cctgcagaac
     3301 tacggtctgg tcgtggatgg ccgcattgcc ctctgctcgg agaactgcga ggagttcttc
     3361 attcccgtga tcgctggtct gttcatcgga gtcggcgttg ccccaaccaa cgagatctac
     3421 accctgcgcg agctggtcca cagcctgggt atcagcaagc cgaccatcgt tttctcgagc
     3481 aagaagggcc tcgacaaggt tatcaccgtg caaaaaaccg tcaccaccat caagaccatc
     3541 gttatcctgg attccaaggt tgactaccgc ggttaccagt gcctcgacac cttcatcaag
     3601 cgtaacaccc cacccggctt ccaagcctcc tccttcaaaa ccgtggaggt tgatcgcaag
     3661 gagcaggtgg ccctgatcat gaactcctcc ggctccaccg gtctgccaaa gggcgtgcag
     3721 ctgacccacg agaacaccgt cacccgcttc tcgcatgccc gtgatcccat ctacggcaac
     3781 caggtgtccc caggcaccgc cgtcctgacc gtcgtgcccc tccaccacgg cttcggcatg
     3841 ttcaccactc tcggctactt gatctgcggc ttccgtgtgg tgatgctgac taagttcgac
     3901 gaggagactt tcctcaagac cctgcaagac tacaagtgca cttacgtgat cctggtgccg
     3961 accctgttcg ccatcctgaa caagagcgag ctgttgaaca aatacgacct gtcgaacctg
     4021 gtggagatcg cttccggcgg cgccccactg tcgaaggaag ttggcgaggc cgttgctcgc
     4081 cgcttcaacc tgcccggcgt ccgtcagggt tacggcctga ccgagactac ctcggctatc
     4141 atcatcaccc ccgagggcga cgataagccc ggcgcttccg gcaaggttgt tccactcttc
     4201 aaagccaagg tgatcgacct ggacaccaag aagtccctgg gtcccaaccg tcgcggagaa
     4261 gtgtgcgtca agggccccat gctgatgaag ggctacgtca acaacccaga ggccaccaag
     4321 gagttgatcg acgaggaggg ctggctgcac actggtgaca tcggttacta cgacgaagag
     4381 aagcacttct tcatcgttga ccgcctgaag tccttgatca agtacaaggg ctaccaggtt
     4441 ccccccgccg agctcgagag cgtcctgctg cagcacccct ccattttcga tgccggagtg
     4501 gccggtgtcc ccgatcccgt ggctggcgag ctgccaggtg ccgtcgtcgt gctggagtcc
     4561 ggcaagaaca tgaccgagaa ggaagtgatg gattacgtgg cctcgcaagt gtccaacgcc
     4621 aagcgcctgc gcggcggcgt gcgcttcgtg gatgaggtgc ccaagggcct caccggaaag
     4681 atcgacggtc gcgctatccg cgagatcctc aagaagcccg tggccaagat gtgagcttgg
     4741 aatggatctt cgatcccgat cgttcaaaca tttggcaata aagtttctta agattgaatc
     4801 ctgttgccgg tcttgcgacg attatcatat aatttctgtt gaattacgtt aagcatgtaa
     4861 taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc
     4921 aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat
     4981 cgcgcgcggt gtcatctatg ttactagatc gggaattgcc aagctaattc ttgaagatga
     5041 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     5101 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     5161 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatgg
     5221 gaccgactcg cgctgtcatg agaccggatc ctgacaggat atattggcgg gtaaactaag
     5281 tcgctgtatg tgtttgtttg agatctcatg tgagcaaaag gccagcaaaa ggccaggaac
     5341 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     5401 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     5461 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     5521 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     5581 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     5641 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     5701 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     5761 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     5821 atctgcgctc tgctgaagcc agttaccttc ggaagaagag ttggtagctc ttgatccggc
     5881 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     5941 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     6001 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     6061 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgtgta acattggtct
     6121 agtgattatt tgccgactac cttggtgatc tcgcctttca cgtagtggac aaattcttcc
     6181 aactgatctg cgcgcgaggc caagcgatct tcttcttgtc caagataagc ctgtctagct
     6241 tcaagtatga cgggctgata ctgggccggc aggcgctcca ttgcccagtc ggcagcgaca
     6301 tccttcggcg cgattttgcc ggttactgcg ctgtaccaaa tgcgggacaa cgtaagcact
     6361 acatttcgct catcaccagc ccagtcgggc ggcgagttcc atagcgttaa ggtttcattt
     6421 agcgcctcaa atagatcctg ttcaggaacc ggatcaaaga gttcctccgc cgctggacct
     6481 accaaggcaa cgctatgttc tcttgctttt gtcagcaaga tagccagatc aatgtcgatc
     6541 gtggctggct cgaagatacc tgcaagaatg tcattgcgct gccattctcc aaattgcagt
     6601 tcgcgcttag ctggataacg ccacggaatg atgtcgtcgt gcacaacaat ggtgacttct
     6661 acagcgcgga gaatctcgct ctctccaggg gaagccgaag tttccaaaag gtcgttgatc
     6721 aaagctcgcc gcgttgtttc atcaagcctt acggtcaccg taaccagcaa atcaatatca
     6781 ctgtgtggct tcaggccgcc atccactgcg gagccgtaca aatgtacggc cagcaacgtc
     6841 ggttcgagat ggcgctcgat gacgccaact acctctgata gttgagtcga tacttcggcg
     6901 atcaccgctt ccctcataat gtttaacttt gttttagggc gactgccctg ctgcgtaaca
     6961 tcgttgctgc tccataacat caaacatcga cccacggcgt aacgcgcttg ctgcttggat
     7021 gcccgaggca tagactgtac cccaaaaaaa cagtcataac aagccatgaa aaccgccact
     7081 gcgccgttac caccgctgcg ttcggtcaag gttctggacc agttgcgtga gcgcatacgc
     7141 tacttgcatt acagcttacg aaccgaacag gcttatgtcc actgggttcg tgccttcatc
     7201 cgtttccacg gtgtgcgtca cccggcaacc ttgggtagca gcgaagtcga ggcatttctg
     7261 tcctggctgg aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg
     7321 atgatatatt tttatcttgt gcaatgtaac atcagagatt ttgagacaca acgtggcttt
     7381 gttgaataaa tcgaactttt gctgagttga aggatcagat cacgcatctt cccgacaacg
     7441 cagaccgttc cgtggcaaag caaaagttca aaatcaccaa ctggtccacc tacaacaaag
     7501 ctctcatcaa ccgtggctcc ctcactttct ggctggatga tggggcgatt caggcgatcc
     7561 ccatccaaca gcccgccgtc gagcgggctt ttttatcccc ggaagcctgt ggatagaggg
     7621 tagttatcca cgtgaaaccg ctaatgcccc gcaaagcctt gattcacggg gctttccggc
     7681 ccgctccaaa aactatccac gtgaaatcgc taatcagggt acgtgaaatc gctaatcgga
     7741 gtacgtgaaa tcgctaataa ggtcacgtga aatcgctaat caaaaaggca cgtgagaacg
     7801 ctaatagccc tttcagatca acagcttgca aacacccctc gctccggcaa gtagttacag
     7861 caagtagtat gttcaattag cttttcaatt atgaatatat atatcaatta ttggtcgccc
     7921 ttggcttgtg gacaatgcgc tacgcgcacc ggctccgccc gtggacaacc gcaagcggtt
     7981 gcccaccgtc gagcgccttt gcccacaacc cggcggccgg ccgcaacaga tcgttttata
     8041 aatttttttt tttgaaaaag aaaaagcccg aaaggcggca acctctcggg cttctggatt
     8101 tccgatcccc ggaattagat cttggcagga tatattgtgg tg
//
