LOCUS       sequence-334536-        9153 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpresses KCNS2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9153)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 9153)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_KCNS2_OE_1
FEATURES             Location/Qualifiers
     source          1..9153
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        24..327
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        328..531
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     482..502
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    554..578
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(1013..1024)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             2036..2077
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2119..2707
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2172..2192)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2590..2601)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2779..3012
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3090..3211
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3127..3146)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3181..3200
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3251..3386
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3406..3425)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3407..3425)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3435..3452)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3435..3451)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3444..3466)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3593..4048
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3680..3699)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3890..3911
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4074..4178
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4179..4247,4248..5039)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4397..4416)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5210..5798
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5699..5718
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5952..5969
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6086..6107
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6122..6127,6128..6145,6146..6152)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6160..6176
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6165..6187
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6184..6200
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6184..6200
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6219..6239
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6221..6239
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6267..6493
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6494..6674
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6721..6846
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7339..7572
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7757..7801
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7976..8476
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8395..8413
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8488..8883
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8943..9060
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
       61 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      121 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
      181 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      241 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      301 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
      361 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
      421 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
      481 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta
      541 actgtcggga tcaacaagtt tgtacaaaaa acttggcatg accggccaga gcctgtggga
      601 cgtgtcggag gctaacgtcg aggacgggga gatccgcatc aatgtgggcg gcttcaagag
      661 gaggctgcgc tcgcacacgc tgctgcgctt ccccgagacg cgcctgggcc gcttgctgct
      721 ctgccactcg cgcgaggcca ttctggagct ctgcgatgac tacgacgacg tccagcggga
      781 gttctacttc gaccgcaacc ctgagctctt cccctacgtg ctgcatttct atcacaccgg
      841 caagcttcac gtcatggctg agctatgtgt cttctccttc agccaggaga tcgagtactg
      901 gggcatcaac gagttcttca ttgactcctg ctgcagctac agctaccatg gccgcaaagt
      961 agagcccgag caggagaagt gggacgagca gagtgaccag gagagcacca cgtcttcctt
     1021 cgatgagatc cttgccttct acaacgacgc ctccaagttc gatgggcagc ccctcggcaa
     1081 cttccgcagg cagctgtggc tggcgctgga caaccccggc tactcagtgc tgagcagggt
     1141 cttcagcatc ctgtccatcc tggtggtgat ggggtccatc atcaccatgt gcctcaatag
     1201 cctgcccgat ttccaaatcc ctgacagcca gggcaaccct ggcgaggacc ctaggttcga
     1261 aatcgtggag cactttggca ttgcctggtt cacatttgag ctggtggcca ggtttgctgt
     1321 ggcccctgac ttcctcaagt tcttcaagaa tgccctaaac cttattgacc tcatgtccat
     1381 cgtccccttt tacatcactc tggtggtgaa cctggtggtg gagagcacac ctactttagc
     1441 caacttgggc agggtggccc aggtcctgag gctgatgcgg atcttccgca tcttaaagct
     1501 ggccaggcac tccactggcc tccgctccct gggggccact ttgaaataca gctacaaaga
     1561 agtagggctg ctcttgctct acctctccgt ggggatttcc atcttctccg tggtggccta
     1621 caccattgaa aaggaggaga acgagggcct ggccaccatc cctgcctgct ggtggtgggc
     1681 taccgtcagt atgaccacag tggggtacgg ggatgtggtc ccagggacca cggcaggaaa
     1741 gctgactgcc tctgcctgca tcttggcagg catcctcgtg gtggtcctgc ccatcacctt
     1801 gatcttcaat aagttctccc acttttaccg gcgccaaaag caacttgaga gtgccatgcg
     1861 cagctgtgac tttggagatg gaatgaagga ggtcccttcg gtcaatttaa gggactatta
     1921 tgcccataaa gttaaatccc ttatggcaag cctgacgaac atgagcagga gctcaccaag
     1981 tgaactcagt ttaaatgatt ccctacgtta ccaaactttc ttgtacaaag tggttggtaa
     2041 gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta gcgctaaccg
     2101 gtggcgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     2161 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     2221 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     2281 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     2341 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     2401 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     2461 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     2521 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     2581 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     2641 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     2701 tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     2761 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     2821 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     2881 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     2941 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     3001 aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg
     3061 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     3121 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     3181 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     3241 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     3301 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     3361 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     3421 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     3481 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     3541 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     3601 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     3661 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     3721 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     3781 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     3841 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     3901 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     3961 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     4021 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     4081 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     4141 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     4201 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     4261 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     4321 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     4381 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     4441 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     4501 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     4561 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     4621 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     4681 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     4741 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     4801 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     4861 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     4921 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     4981 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     5041 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     5101 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     5161 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     5221 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     5281 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     5341 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     5401 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     5461 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     5521 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     5581 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     5641 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     5701 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     5761 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     5821 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     5881 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     5941 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     6001 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     6061 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     6121 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     6181 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
     6241 aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa
     6301 catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg
     6361 attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac
     6421 atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc
     6481 tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     6541 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     6601 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     6661 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     6721 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     6781 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     6841 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     6901 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     6961 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     7021 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     7081 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     7141 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
     7201 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     7261 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     7321 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     7381 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     7441 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     7501 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     7561 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     7621 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     7681 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     7741 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     7801 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     7861 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     7921 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccttgcg
     7981 ccttttccaa ggcagccctg ggtttgcgca gggacgcggc tgctctgggc gtggttccgg
     8041 gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc acgtccgttc gcagcgtcac
     8101 ccggatcttc gccgctaccc ttgtgggccc cccggcgacg cttcctgctc cgcccctaag
     8161 tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct
     8221 cactagtacc ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg
     8281 ggctgtggcc aatagcggct gctcagcagg gcgcgccgag agcagcggcc gggaaggggc
     8341 ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc
     8401 cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc tccctcgttg accgaatcac
     8461 cgacctctct ccccaggggg taccaccatg gccaagcctt tgtctcaaga agaatccacc
     8521 ctcattgaaa gagcaacggc tacaatcaac agcatcccca tctctgaaga ctacagcgtc
     8581 gccagcgcag ctctctctag cgacggccgc atcttcactg gtgtcaatgt atatcatttt
     8641 actgggggac cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc ggcagctggc
     8701 aacctgactt gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag cccctgcgga
     8761 cggtgccgac aggtgcttct cgatctgcat cctgggatca aagccatagt gaaggacagt
     8821 gatggacagc cgacggcagt tgggattcgt gaattgctgc cctctggtta tgtgtgggag
     8881 ggcctgcagc tgcagtagta agaattctag atcttgagac aaatggcagt attcatccac
     8941 aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     9001 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     9061 cgggtttatt acagggacag cagagatcca ctttggcgcc ggctcgaggg ggcccggggt
     9121 tattaatagt aatcaattac ggggtcatta gtt
//
