LOCUS       sequence-334541-        9642 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpresses SCNN1B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9642)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 9642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_SCNN1B_OE_1
FEATURES             Location/Qualifiers
     source          1..9642
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        81..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        385..588
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     539..559
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    611..635
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2556..2580)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2582..2623
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2665..3253
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2718..2738)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3136..3147)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3325..3558
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3636..3757
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3673..3692)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3727..3746
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3797..3932
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3952..3971)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3953..3971)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3981..3998)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3981..3997)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3990..4012)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4139..4594
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4226..4245)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4436..4457
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4620..4724
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4725..4793,4794..5585)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4943..4962)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5756..6344
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6245..6264
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6498..6515
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6632..6653
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6668..6673,6674..6691,6692..6698)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6706..6722
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6711..6733
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6730..6746
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6730..6746
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6765..6785
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6767..6785
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6813..7039
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7040..7220
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7267..7392
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7885..8118
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8303..8347
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8522..9022
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8941..8959
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             9034..9429
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    9489..9606
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgccggctcg agggggcccg gggttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
      421 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
      481 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
      541 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact
      601 gtcgggatca acaagtttgt acaaaaaagt tggcatgcac gtgaagaagt acctgctgaa
      661 gggcctgcat cggctgcaga agggccccgg ctacacgtac aaggagctgc tggtgtggta
      721 ctgcgacaac accaacaccc acggccccaa gcgcaccatc tgtgaggggc ccaagaagaa
      781 agccatgtgg ttcctgctca ccctgctctt cgccgccctc gtctgctggc agtggggcat
      841 cttcatcagg acctacttga gctgggaggt cagcgtctcc ctctccgtag gcttcaagac
      901 catggacttc cctgccgtca ccatctgcaa tgctagcccc ttcaagtatt ccaaaatcaa
      961 gcatttgctg aaggacctgg atgagctgat ggaagctgtc ctggagagaa tcctggctcc
     1021 tgagctaagc catgccaatg ccaccaggaa cctgaacttc tccatctgga accacacacc
     1081 cctggtcctt attgatgaac ggaaccccca ccaccccatg gtccttgatc tctttggaga
     1141 caaccacaat ggcttaacaa gcagctcagc atcagaaaag atctgtaatg cccacgggtg
     1201 caaaatggcc atgagactat gtagcctcaa caggacccag tgtaccttcc ggaacttcac
     1261 cagtgctacc caggcattga cagagtggta catcctgcag gccaccaaca tctttgcaca
     1321 ggtgccacag caggagctag tagagatgag ctaccccggc gagcagatga tcctggcctg
     1381 cctattcgga gctgagccct gcaactaccg gaacttcacg tccatcttct accctcacta
     1441 tggcaactgt tacatcttca actggggcat gacagagaag gcacttcctt cggccaaccc
     1501 tggaactgaa ttcggcctga agttgatcct ggacataggc caggaagact acgtcccctt
     1561 ccttgcgtcc acggccgggg tcaggctgat gcttcacgag cagaggtcat accccttcat
     1621 cagagatgag ggcatctacg ccatgtcggg gacagagacg tccatcgggg tactcgtgga
     1681 caagcttcag cgcatggggg agccctacag cccgtgcacc gtgaatggtt ctgaggtccc
     1741 cgtccaaaac ttctacagtg actacaacac gacctactcc atccaggcct gtcttcgctc
     1801 ctgcttccaa gaccacatga tccgtaactg caactgtggc cactacctgt acccactgcc
     1861 ccgtggggag aaatactgca acaaccggga cttcccagac tgggcccatt gctactcaga
     1921 tctacagatg agcgtggcgc agagagagac ctgcattggc atgtgcaagg agtcctgcaa
     1981 tgacacccag tacaagatga ccatctccat ggctgactgg ccttctgagg cctccgagga
     2041 ctggattttc cacgtcttgt ctcaggagcg ggaccaaagc accaatatca ccctgagcag
     2101 gaagggaatt gtcaagctca acatctactt ccaagaattt aactatcgca ccattgaaga
     2161 atcagcagcc aataacatcg tctggctgct ctcgaatctg ggtggccagt ttggcttctg
     2221 gatggggggc tctgtgctgt gcctcatcga gtttggggag atcatcatcg actttgtgtg
     2281 gatcaccatc atcaagctgg tggccttggc caagagccta cggcagcggc gagcccaagc
     2341 cagctacgct ggcccaccgc ccaccgtggc cgagctggtg gaggcccaca ccaactttgg
     2401 cttccagcct gacacggccc cccgcagccc caacactggg ccctacccca gtgagcaggc
     2461 cctgcccatc ccaggcaccc cgccccccaa ctatgactcc ctgcgtctgc agccgctgga
     2521 cgtcatcgag tctgacagtg agggtgatgc catctaccca actttcttgt acaaagtggt
     2581 tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat gagctagcgc
     2641 taaccggtgg cgcgttaagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg
     2701 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     2761 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     2821 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     2881 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     2941 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     3001 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     3061 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     3121 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     3181 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     3241 gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     3301 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     3361 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     3421 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     3481 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     3541 tgtggaaaat ctctagcagt acgtatagta gttcatgtca tcttattatt cagtatttat
     3601 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
     3661 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     3721 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
     3781 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     3841 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     3901 ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt
     3961 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     4021 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     4081 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     4141 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     4201 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     4261 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     4321 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     4381 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     4441 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     4501 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     4561 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
     4621 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     4681 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     4741 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     4801 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     4861 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     4921 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     4981 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     5041 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     5101 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     5161 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     5221 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     5281 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     5341 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     5401 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     5461 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     5521 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     5581 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     5641 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     5701 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     5761 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     5821 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     5881 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     5941 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     6001 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     6061 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     6121 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     6181 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     6241 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     6301 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     6361 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     6421 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     6481 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     6541 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     6601 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     6661 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     6721 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
     6781 aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc
     6841 ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc
     6901 atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg
     6961 tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg
     7021 cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc
     7081 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     7141 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     7201 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
     7261 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
     7321 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
     7381 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
     7441 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
     7501 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
     7561 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
     7621 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
     7681 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat
     7741 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     7801 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     7861 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     7921 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     7981 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     8041 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     8101 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     8161 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     8221 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     8281 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     8341 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     8401 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     8461 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
     8521 cttgcgcctt ttccaaggca gccctgggtt tgcgcaggga cgcggctgct ctgggcgtgg
     8581 ttccgggaaa cgcagcggcg ccgaccctgg gtctcgcaca ttcttcacgt ccgttcgcag
     8641 cgtcacccgg atcttcgccg ctacccttgt gggccccccg gcgacgcttc ctgctccgcc
     8701 cctaagtcgg gaaggttcct tgcggttcgc ggcgtgccgg acgtgacaaa cggaagccgc
     8761 acgtctcact agtaccctcg cagacggaca gcgccaggga gcaatggcag cgcgccgacc
     8821 gcgatgggct gtggccaata gcggctgctc agcagggcgc gccgagagca gcggccggga
     8881 aggggcggtg cgggaggcgg ggtgtggggc ggtagtgtgg gccctgttcc tgcccgcgcg
     8941 gtgttccgca ttctgcaagc ctccggagcg cacgtcggca gtcggctccc tcgttgaccg
     9001 aatcaccgac ctctctcccc agggggtacc accatggcca agcctttgtc tcaagaagaa
     9061 tccaccctca ttgaaagagc aacggctaca atcaacagca tccccatctc tgaagactac
     9121 agcgtcgcca gcgcagctct ctctagcgac ggccgcatct tcactggtgt caatgtatat
     9181 cattttactg ggggaccttg tgcagaactc gtggtgctgg gcactgctgc tgctgcggca
     9241 gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc
     9301 tgcggacggt gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc catagtgaag
     9361 gacagtgatg gacagccgac ggcagttggg attcgtgaat tgctgccctc tggttatgtg
     9421 tgggagggcc tgcagctgca gtagtaagaa ttctagatct tgagacaaat ggcagtattc
     9481 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     9541 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     9601 aattttcggg tttattacag ggacagcaga gatccacttt gg
//
