LOCUS       sequence-334543-        9891 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpresses TMC7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9891)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 9891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_TMC7_OE_1
FEATURES             Location/Qualifiers
     source          1..9891
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        56..359
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        360..563
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     514..534
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    586..610
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2780..2804)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2806..2847
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2889..3477
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2942..2962)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3360..3371)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3549..3782
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3860..3981
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3897..3916)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3951..3970
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4021..4156
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4176..4195)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4177..4195)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4205..4222)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4205..4221)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4214..4236)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4363..4818
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4450..4469)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4660..4681
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4844..4948
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4949..5017,5018..5809)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5167..5186)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5980..6568
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6469..6488
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6722..6739
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6856..6877
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6892..6897,6898..6915,6916..6922)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6930..6946
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6935..6957
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6954..6970
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6954..6970
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6989..7009
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6991..7009
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7037..7263
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7264..7444
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7491..7616
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8109..8342
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8527..8571
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8746..9246
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     9165..9183
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             9258..9653
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    9713..9830
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
       61 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
      121 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
      181 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
      241 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
      301 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
      361 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
      421 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      481 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      541 gggaggtcta tataagcaga gctctctggc taactgtcgg gatcaacaag tttgtacaaa
      601 aaagttggca tgagcgagtc cagcggcagt gcgctccagc ccggcaggcc cagccggcag
      661 ccggcggtcc atccagagaa cctctctcta gactccagtt gcttctcttc tccacctgtg
      721 aacttcctcc aagaattgcc aagctaccgg tccattgcac gtaggagaac gactgtccat
      781 tcctgggaca agcaaagcgg aactttgcta aagccaaccg actcttacag ctcccagctg
      841 gaggacagaa tcgctgaaaa cctcagcagc cattctcttc gaaattatgc actgaacatc
      901 tctgagaagc ggagactaag ggacattcaa gagacacaaa tgaagtatct ctccgaatgg
      961 gaccagtgga agcggtatag cagcaagtct tggaagaggt tcctagagaa ggctcgagag
     1021 atgacgaccc acctggagct gtggcgggag gacatccgca gcatagaagg gaaatttggc
     1081 actgggattc agtcctattt ctccttcttg agattcctgg tgttgctgaa tttggtgata
     1141 tttctgatca tctttatgct ggttttgctc ccagtcttac tcacgaaata caagatcacc
     1201 aacagcagct tcgtgctcat tcctttcaaa gacatggata aacaatgtac agtctatcca
     1261 gtaagcagtt ctggactcat ttacttttac agttatatca tagacttgct ttctggcact
     1321 ggtttcctgg aggaaactag cctcttttac ggacattaca ccattgatgg ggtgaaattt
     1381 cagaacttca cctatgatct gcccctggcg tatttgttaa gcacaatcgc ctccctggcc
     1441 ctgagccttc tttggatagt gaaaaggtcg gtggaaggat tcaaaatcaa cctgattcgg
     1501 agtgaggagc actttcagag ttactgcaac aagatatttg ccggctggga cttctgcatc
     1561 actaaccgca gcatggcgga tctgaagcac agcagcttgc ggtacgagct ccgagcagat
     1621 ctggaggaag aaagaatgcg gcagaaaata gcagaaagga cctcagaaga aacaatacgc
     1681 atttactctt tgagactgtt tttgaactgt attgttctgg ctgttttagg ggcatgcttt
     1741 tatgcaatat acgtagcaac tgtcttctcg caagagcaca tgaaaaagga aatcgacaag
     1801 atggtttttg gagagaacct cttcatattg tatctaccgt ctattgtgat cacgctggcc
     1861 aattttatca ccccaatgat ctttgccaag atcatccgct atgaggatta ttctccaggc
     1921 tttgagatcc gtctgacaat ccttaggtgt gtctttatgc ggctggccac catatgtgtc
     1981 ctggtgttca cgctgggctc caagatcaca tcctgtgatg atgacacatg tgacctttgc
     2041 ggctacaacc agaaactcta cccgtgctgg gagacccaag ttgggcagga aatgtacaag
     2101 ctgatgatct tcgacttcat catcatcttg gctgtgacac tcttcgtgga ttttcctaga
     2161 aagctcctgg tgacctactg ttcctcttgc aagctgattc agtgctgggg gcagcaggag
     2221 tttgccattc ctgataacgt cctggggata gtttacgggc aaaccatctg ctggatcgga
     2281 gcctttttct caccccttct ccctgcaatt gcaaccctga aattcattat catcttctat
     2341 gtgaaagagt ggagtctgct ttacacctgc agaccctccc ccaggccgtt cagagcatcc
     2401 aattctaatt tcttcttcct gttggtgttg ttgatcgggc tgtgtttggc aataatacct
     2461 ctgacaatca gcatatcacg catcccttcc tcgaaagcct gtgggccgtt caccaacttc
     2521 aacaccacct gggaggtcat ccccaagaca gtgagcacct tccccagctc gctgcagtcc
     2581 ttcatccatg gtgtcacatc cgaagccttt gcagttcctt tcttcatgat tatttgcctc
     2641 atcatgtttt acttcattgc cttagctgga gcacacaaac gggtggtcat ccagctccga
     2701 gagcagctat ccctggaaag tcgtgacaag tgctacctaa tccagaaact aacagaagcc
     2761 caaagggaca tgaggaacta cccaactttc ttgtacaaag tggttggtaa gcctatccct
     2821 aaccctctcc tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt
     2881 aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     2941 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     3001 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     3061 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     3121 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     3181 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     3241 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     3301 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     3361 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     3421 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcgtc
     3481 gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     3541 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac
     3601 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     3661 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     3721 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     3781 cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     3841 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
     3901 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     3961 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
     4021 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     4081 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     4141 ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc
     4201 gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     4261 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     4321 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg
     4381 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     4441 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     4501 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     4561 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     4621 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     4681 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     4741 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     4801 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     4861 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     4921 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     4981 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     5041 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     5101 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     5161 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     5221 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     5281 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     5341 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     5401 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     5461 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     5521 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     5581 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     5641 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     5701 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     5761 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     5821 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     5881 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     5941 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     6001 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     6061 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     6121 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     6181 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     6241 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     6301 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     6361 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     6421 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     6481 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     6541 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     6601 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     6661 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     6721 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     6781 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     6841 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     6901 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     6961 cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct
     7021 ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg
     7081 atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa
     7141 gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga
     7201 cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata
     7261 aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     7321 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     7381 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     7441 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
     7501 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
     7561 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
     7621 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     7681 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     7741 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     7801 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     7861 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     7921 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
     7981 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     8041 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     8101 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     8161 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     8221 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     8281 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     8341 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     8401 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     8461 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     8521 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     8581 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     8641 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     8701 tcaccattat cgtttcagac ccacctccca accccgaggg gacccttgcg ccttttccaa
     8761 ggcagccctg ggtttgcgca gggacgcggc tgctctgggc gtggttccgg gaaacgcagc
     8821 ggcgccgacc ctgggtctcg cacattcttc acgtccgttc gcagcgtcac ccggatcttc
     8881 gccgctaccc ttgtgggccc cccggcgacg cttcctgctc cgcccctaag tcgggaaggt
     8941 tccttgcggt tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct cactagtacc
     9001 ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg ggctgtggcc
     9061 aatagcggct gctcagcagg gcgcgccgag agcagcggcc gggaaggggc ggtgcgggag
     9121 gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc cgcattctgc
     9181 aagcctccgg agcgcacgtc ggcagtcggc tccctcgttg accgaatcac cgacctctct
     9241 ccccaggggg taccaccatg gccaagcctt tgtctcaaga agaatccacc ctcattgaaa
     9301 gagcaacggc tacaatcaac agcatcccca tctctgaaga ctacagcgtc gccagcgcag
     9361 ctctctctag cgacggccgc atcttcactg gtgtcaatgt atatcatttt actgggggac
     9421 cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc ggcagctggc aacctgactt
     9481 gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag cccctgcgga cggtgccgac
     9541 aggtgcttct cgatctgcat cctgggatca aagccatagt gaaggacagt gatggacagc
     9601 cgacggcagt tgggattcgt gaattgctgc cctctggtta tgtgtgggag ggcctgcagc
     9661 tgcagtagta agaattctag atcttgagac aaatggcagt attcatccac aattttaaaa
     9721 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     9781 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     9841 acagggacag cagagatcca ctttggcgcc ggctcgaggg ggcccggggt t
//
