LOCUS       sequence-334567-        9090 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpresses KCNA7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9090)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 9090)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_KCNA7_OE_1
FEATURES             Location/Qualifiers
     source          1..9090
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..524
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     443..461
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             536..931
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    991..1108
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1225..1528
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1529..1732
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1683..1703
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    1755..1779
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3148..3172)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3174..3215
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3257..3845
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3310..3330)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3728..3739)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3917..4150
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4228..4349
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4265..4284)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4319..4338
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4389..4524
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4544..4563)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4545..4563)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4573..4590)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4573..4589)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4582..4604)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4731..5186
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4818..4837)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5028..5049
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5212..5316
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5317..5385,5386..6177)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5535..5554)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6348..6936
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6837..6856
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7090..7107
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7224..7245
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7260..7265,7266..7283,7284..7290)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7298..7314
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7303..7325
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7322..7338
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7322..7338
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7357..7377
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7359..7377
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7405..7631
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7632..7812
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7859..7984
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8477..8710
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8895..8939
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 acctcccaac cccgagggga cccttgcgcc ttttccaagg cagccctggg tttgcgcagg
       61 gacgcggctg ctctgggcgt ggttccggga aacgcagcgg cgccgaccct gggtctcgca
      121 cattcttcac gtccgttcgc agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc
      181 cggcgacgct tcctgctccg cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc
      241 ggacgtgaca aacggaagcc gcacgtctca ctagtaccct cgcagacgga cagcgccagg
      301 gagcaatggc agcgcgccga ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc
      361 gcgccgagag cagcggccgg gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt
      421 gggccctgtt cctgcccgcg cggtgttccg cattctgcaa gcctccggag cgcacgtcgg
      481 cagtcggctc cctcgttgac cgaatcaccg acctctctcc ccagggggta ccaccatggc
      541 caagcctttg tctcaagaag aatccaccct cattgaaaga gcaacggcta caatcaacag
      601 catccccatc tctgaagact acagcgtcgc cagcgcagct ctctctagcg acggccgcat
      661 cttcactggt gtcaatgtat atcattttac tgggggacct tgtgcagaac tcgtggtgct
      721 gggcactgct gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga tcggaaatga
      781 gaacaggggc atcttgagcc cctgcggacg gtgccgacag gtgcttctcg atctgcatcc
      841 tgggatcaaa gccatagtga aggacagtga tggacagccg acggcagttg ggattcgtga
      901 attgctgccc tctggttatg tgtgggaggg cctgcagctg cagtagtaag aattctagat
      961 cttgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
     1021 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
     1081 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact
     1141 ttggcgccgg ctcgaggggg cccggggtta ttaatagtaa tcaattacgg ggtcattagt
     1201 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
     1261 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
     1321 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
     1381 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
     1441 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
     1501 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
     1561 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
     1621 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
     1681 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctctctggct
     1741 aactgtcggg atcaacaagt ttgtacaaaa aagcaggcac catggagccg cggtgcccgc
     1801 cgccgtgcgg ctgctgcgag cggctggtgc tcaacgtggc cgggctgcgc ttcgagacgc
     1861 gggcgcgcac gctgggccgc ttcccggaca ctctgctagg ggacccagcg cgccgcggcc
     1921 gcttctacga cgacgcgcgc cgcgagtatt tcttcgaccg gcaccggccc agcttcgacg
     1981 ccgtgctcta ctactaccag tccggtgggc ggctgcggcg gccggcgcac gtgccgctcg
     2041 acgtcttcct ggaagaggtg gccttctacg ggctgggcgc ggcggccctg gcacgcctgc
     2101 gcgaggacga gggctgcccg gtgccgcccg agcgccccct gccccgccgc gccttcgccc
     2161 gccagctgtg gctgcttttc gagtttcccg agagctctca ggccgcgcgc gtgctcgccg
     2221 tagtctccgt gctggtcatc ctcgtctcca tcgtcgtctt ctgcctcgag acgctgcctg
     2281 acttccgcga cgaccgcgac ggcacggggc ttgctgctgc agccgcagcc ggcccgttcc
     2341 ccgctcggct gaatggctcc agccaaatgc ctggaaatcc accccgcctg cccttcaatg
     2401 acccgttctt cgtggtggag acgctgtgta tttgttggtt ctcctttgag ctgctggtac
     2461 gcctcctggt ctgtccaagc aaggctatct tcttcaagaa cgtgatgaac ctcatcgatt
     2521 ttgtggctat ccttccctac tttgtggcac tgggcaccga gctggcccgg cagcgagggg
     2581 tgggccagca ggccatgtca ctggccatcc tgagagtcat ccgattggtg cgtgtcttcc
     2641 gcatcttcaa gctgtcccgg cactcaaagg gcctgcaaat cttgggccag acgcttcggg
     2701 cctccatgcg tgagctgggc ctcctcatct ttttcctctt catcggtgtg gtcctctttt
     2761 ccagcgccgt ctactttgcc gaagttgacc gggtggactc ccatttcact agcatccctg
     2821 agtccttctg gtgggcggta gtcaccatga ctacagttgg ctatggagac atggcacccg
     2881 tcactgtggg tggcaagata gtgggctctc tgtgtgccat tgcgggcgtg ctgactattt
     2941 ccctgccagt gcccgtcatt gtctccaatt tcagctactt ttatcaccgg gagacagagg
     3001 gcgaagaggc tgggatgttc agccatgtgg acacgcagcc ttgtggccca ctggagggca
     3061 aggccaatgg ggggctggtg gacggggagg tacctgagct accacctcca ctctgggcac
     3121 ccccagggaa acacctggtc accgaagtga cagctttctt gtacaaagtg gttggtaagc
     3181 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt
     3241 ggcgcgttaa gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     3301 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     3361 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     3421 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     3481 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     3541 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     3601 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     3661 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     3721 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     3781 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     3841 cccgcgtcga ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     3901 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt
     3961 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     4021 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     4081 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     4141 atctctagca gtacgtatag tagttcatgt catcttatta ttcagtattt ataacttgca
     4201 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
     4261 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     4321 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa
     4381 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     4441 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     4501 agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta
     4561 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     4621 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     4681 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg
     4741 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     4801 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     4861 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     4921 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     4981 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     5041 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     5101 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     5161 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc
     5221 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     5281 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     5341 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     5401 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     5461 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     5521 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     5581 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     5641 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     5701 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     5761 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     5821 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     5881 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     5941 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     6001 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     6061 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     6121 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     6181 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     6241 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     6301 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     6361 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     6421 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     6481 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     6541 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     6601 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     6661 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     6721 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     6781 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     6841 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     6901 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     6961 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     7021 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     7081 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     7141 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     7201 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     7261 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     7321 acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa
     7381 caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca
     7441 tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat
     7501 tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat
     7561 ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc
     7621 gatacataaa cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     7681 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     7741 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     7801 aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct
     7861 ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact
     7921 ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag
     7981 cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg
     8041 gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt
     8101 cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct
     8161 acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac
     8221 cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat
     8281 agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac
     8341 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
     8401 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
     8461 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
     8521 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
     8581 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
     8641 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
     8701 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
     8761 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
     8821 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
     8881 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
     8941 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
     9001 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
     9061 agggatattc accattatcg tttcagaccc
//
