LOCUS       sequence-334569-        9354 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpresses KCNN1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9354)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 9354)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_KCNN1_OE_1
FEATURES             Location/Qualifiers
     source          1..9354
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        46..349
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        350..553
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    576..600
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2233..2257)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2259..2300
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2342..2930
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2395..2415)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2813..2824)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3002..3235
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3313..3434
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3350..3369)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3404..3423
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3474..3609
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3629..3648)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3630..3648)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3658..3675)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3658..3674)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3667..3689)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3816..4271
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3903..3922)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4113..4134
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4297..4401
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4402..4470,4471..5262)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4620..4639)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5433..6021
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5922..5941
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6175..6192
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6309..6330
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6345..6350,6351..6368,6369..6375)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6383..6399
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6388..6410
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6407..6423
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6407..6423
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6442..6462
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6444..6462
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6490..6716
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6717..6897
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6944..7069
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7562..7795
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7980..8024
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8199..8699
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8618..8636
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8711..9106
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    9166..9283
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
       61 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
      121 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
      181 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
      241 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
      301 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
      361 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
      421 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
      481 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
      541 tataagcaga gctctctggc taactgtcgg gatcaacaag tttgtacaaa aaagttggca
      601 ccatgaacag ccacagctac aatggcagcg tggggcggcc gctgggcagc gggccgggcg
      661 ccctgggacg agaccctccg gaccctgagg ccggccaccc cccacaaccc ccgcacagcc
      721 cgggcctcca ggtggtagtg gccaagagtg agccagcccg gccctcaccc ggcagccccc
      781 gggggcagcc ccaggaccag gacgatgacg aggatgatga ggaagatgag gccggcaggc
      841 agagagcctc ggggaaaccc tcaaatgtgg gccaccgcct gggccaccgg cgggcgctct
      901 tcgagaagcg gaagcgcctc agcgactatg ccctcatttt cggcatgttt ggcatcgtcg
      961 tcatggtgac ggagaccgag ctgtcctggg gggtgtacac caaggagtct ctgtactcat
     1021 tcgcactcaa atgcctcatc agcctctcca cggccatcct gctgggtctc gttgtcctct
     1081 accatgcccg ggagatccag ctgttcatgg tggacaacgg ggctgatgac tggcgcatcg
     1141 ccatgacctg cgagcgcgtg ttcctcatct cgctagagct ggcagtgtgc gccattcacc
     1201 cggtgcccgg ccactaccgc ttcacgtgga cggcgcggct ggccttcacg tacgcgccct
     1261 cggtggccga ggccgacgtg gacgtgctgc tgtccatccc catgttcctg cgcctctacc
     1321 tgctgggccg ggtgatgcta ctgcacagca aaatcttcac ggacgcctcg agccgcagca
     1381 tcggggccct caacaagatc accttcaaca cgcgcttcgt catgaagaca ctcatgacca
     1441 tctgccccgg caccgtgctg ctggtcttca gcatctcctc ctggatcatc gcagcctgga
     1501 ccgtgcgcgt ctgcgagagg taccacgaca agcaggaagt gaccagcaac ttcctggggg
     1561 ccatgtggct gatttccatc accttcctct ccattggcta cggcgacatg gtgccccaca
     1621 cctactgcgg gaagggtgtg tgcctgctca ctggcatcat gggagctggc tgtaccgcgc
     1681 tcgtggtggc tgtggtggct cggaagctgg agctcaccaa ggctgagaag cacgtgcaca
     1741 acttcatgat ggacactcag ctcaccaagc gggtaaaaaa cgccgctgct aacgttctca
     1801 gggagacgtg gctcatctac aaacatacca ggctggtgaa gaagccagac caagcccggg
     1861 ttcggaaaca ccagcgtaag ttcctccaag ccatccatca ggctcagaag ctccggagtg
     1921 tgaagatcga gcaagggaag ctgaacgacc aggctaacac gcttaccgac ctagccaaga
     1981 cccagaccgt catgtacgac cttgtatcgg agctgcacgc tcagcacgag gagctggagg
     2041 cccgcctggc caccctggaa agccgcttgg atgcgctggg tgcctctcta caggccctgc
     2101 ctggcctcat cgcccaagcc atacgcccac ccccgcctcc cctgcctccc aggcccggcc
     2161 ccggccccca agaccaggca gcccggagct ccccctgccg gtggacgccc gtggccccct
     2221 cggactgcgg gttgccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc
     2281 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga
     2341 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     2401 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     2461 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     2521 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     2581 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     2641 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     2701 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     2761 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     2821 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     2881 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc gtcgacttta
     2941 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     3001 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     3061 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     3121 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     3181 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtacg
     3241 tatagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     3301 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     3361 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     3421 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc
     3481 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     3541 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     3601 ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact
     3661 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     3721 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     3781 ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag
     3841 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     3901 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     3961 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     4021 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     4081 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     4141 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     4201 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     4261 gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     4321 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     4381 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     4441 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     4501 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     4561 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     4621 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     4681 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     4741 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     4801 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     4861 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     4921 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     4981 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     5041 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     5101 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     5161 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     5221 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     5281 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     5341 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     5401 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     5461 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     5521 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     5581 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     5641 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     5701 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     5761 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     5821 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     5881 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     5941 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     6001 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     6061 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     6121 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     6181 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     6241 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     6301 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     6361 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     6421 gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg
     6481 caagcttaat gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt
     6541 agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt
     6601 ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca
     6661 ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt
     6721 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     6781 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     6841 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
     6901 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
     6961 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
     7021 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
     7081 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
     7141 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg
     7201 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
     7261 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
     7321 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
     7381 caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata
     7441 tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag
     7501 gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa
     7561 taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa
     7621 tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt
     7681 tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc
     7741 agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga
     7801 tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga
     7861 gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa
     7921 ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa
     7981 agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca
     8041 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt
     8101 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat
     8161 tatcgtttca gacccacctc ccaaccccga ggggaccctt gcgccttttc caaggcagcc
     8221 ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc cgggaaacgc agcggcgccg
     8281 accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt cacccggatc ttcgccgcta
     8341 cccttgtggg ccccccggcg acgcttcctg ctccgcccct aagtcgggaa ggttccttgc
     8401 ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg tctcactagt accctcgcag
     8461 acggacagcg ccagggagca atggcagcgc gccgaccgcg atgggctgtg gccaatagcg
     8521 gctgctcagc agggcgcgcc gagagcagcg gccgggaagg ggcggtgcgg gaggcggggt
     8581 gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg ttccgcattc tgcaagcctc
     8641 cggagcgcac gtcggcagtc ggctccctcg ttgaccgaat caccgacctc tctccccagg
     8701 gggtaccacc atggccaagc ctttgtctca agaagaatcc accctcattg aaagagcaac
     8761 ggctacaatc aacagcatcc ccatctctga agactacagc gtcgccagcg cagctctctc
     8821 tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc
     8881 agaactcgtg gtgctgggca ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt
     8941 cgcgatcgga aatgagaaca ggggcatctt gagcccctgc ggacggtgcc gacaggtgct
     9001 tctcgatctg catcctggga tcaaagccat agtgaaggac agtgatggac agccgacggc
     9061 agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg gagggcctgc agctgcagta
     9121 gtaagaattc tagatcttga gacaaatggc agtattcatc cacaatttta aaagaaaagg
     9181 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     9241 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     9301 cagcagagat ccactttggc gccggctcga gggggcccgg ggttattaat agta
//
