LOCUS       sequence-334571-        9351 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpresses PANX2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9351)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 9351)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_PANX2_OE_1
FEATURES             Location/Qualifiers
     source          1..9351
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        59..362
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        363..566
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     517..537
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    589..613
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2243..2267)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2269..2310
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2352..2940
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2405..2425)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2823..2834)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3012..3245
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3323..3444
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3360..3379)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3414..3433
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3484..3619
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3639..3658)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3640..3658)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3668..3685)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3668..3684)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3677..3699)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3826..4281
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3913..3932)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4123..4144
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4307..4411
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4412..4480,4481..5272)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4630..4649)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5443..6031
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5932..5951
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6185..6202
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6319..6340
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6355..6360,6361..6378,6379..6385)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6393..6409
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6398..6420
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6417..6433
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6417..6433
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6452..6472
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6454..6472
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6500..6726
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6727..6907
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6954..7079
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7572..7805
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7990..8034
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8209..8709
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8628..8646
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8721..9116
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    9176..9293
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
       61 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      121 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      181 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      241 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      301 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      361 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
      421 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      481 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
      541 ggtgggaggt ctatataagc agagctctct ggctaactgt cgggatcaac aagtttgtac
      601 aaaaaagttg gcaccatgta cgtgcccgcg ctgggctggg agttcctggc ctccacgcgc
      661 ctcacctccg agctcaactt cctgctgcag gagatcgaca actgttacca ccgggcggcc
      721 gagggccgcg cgcccaagat cgagaagcag atccagtcca agggcccggg catcacggag
      781 cgcgagaagc gcgagatcat cgagaacgcg gagaaggaga agagcccgga gcagaacctg
      841 ttcgagaagt acctggagcg ccgcggccgc agcaacttcc tggccaagct gtacctggcg
      901 cggcacgtgc tgatcctgct gctgagcgcc gtgcccatct cctacctgtg cacctactac
      961 gccacgcaga agcagaacga gttcacctgc gcgctgggcg cgtccccgga cggggcggca
     1021 ggtgcggggc ccgcggtgcg cgtgagctgc aagctcccgt ccgtgcaact gcagcgcatc
     1081 atcgcgggcg tggacatcgt gctgctgtgc gtcatgaacc tcatcatcct cgtcaacctc
     1141 atccacctct tcatcttccg caagagcaac ttcatcttcg acaagctgca caaggtgggc
     1201 atcaagacgc gccggcagtg gcgccgctcg cagttctgcg acatcaacat cctggccatg
     1261 ttctgcaacg agaaccgcga ccacatcaag tcgctcaacc ggctggactt catcaccaac
     1321 gagagcgacc tcatgtacga caacgtggtc cggcagctgc tggcggcgct ggcgcagtcc
     1381 aaccacgacg ccacccccac ggtgcgcgac tcgggggtgc agaccgtgga ccccagcgcc
     1441 aaccccgccg agcccgacgg cgccgccgag ccgcccgtgg tcaagcggcc gcgcaagaag
     1501 atgaagtgga tccccaccag caacccgctt ccgcagccct tcaaggagcc gctggccatc
     1561 atgcgcgtgg agaacagcaa ggcggagaag ccgaagcccg cgcgcaggaa gacggccacg
     1621 gacacgctga tcgcgccgct gctggaccgc tccgcccacc actacaaggg cggagggggc
     1681 gacccgggcc ccggccccgc ccctgccccc gccccgccgc ccgcccccga caagaagcac
     1741 gcgcgccact tctccctgga cgtgcacccc tacatcctcg gcaccaagaa ggccaaggcc
     1801 gaggcggtgc ccgccgccct gcccgcctcc cggagccagg aggggggctt cctgtcccag
     1861 gcggaggact gtgggctggg cctggccccg gcgcccatca aagatgctcc gctccccgag
     1921 aaggaaatcc cgtaccccac agagccagcc cgggcagggc ttccctcggg gggcccgttc
     1981 cacgtccgct cacctcccgc cgcccctgct gtggcccctc tgacaccagc cagcctgggc
     2041 aaggcggagc ccctcaccat cctgagccga aacgccacac acccgctgct gcacatcaac
     2101 acgctgtacg aggcccggga ggaggaggac gggggccccc gcctgccgca ggacgtgggg
     2161 gacctcatcg ccatccctgc cccacagcag atcctcatcg ccaccttcga cgagccgaga
     2221 acggtcgtga gtactgtgga gtttccaact ttcttgtaca aagtggttgg taagcctatc
     2281 cctaaccctc tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc
     2341 gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     2401 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     2461 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     2521 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     2581 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     2641 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     2701 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     2761 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     2821 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     2881 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     2941 gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     3001 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg
     3061 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     3121 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     3181 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     3241 tagcagtacg tatagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     3301 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag
     3361 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     3421 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg
     3481 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     3541 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     3601 ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg
     3661 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     3721 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     3781 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg
     3841 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     3901 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     3961 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     4021 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     4081 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     4141 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     4201 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     4261 aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat
     4321 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     4381 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     4441 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     4501 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     4561 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     4621 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     4681 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     4741 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     4801 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     4861 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     4921 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     4981 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     5041 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     5101 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     5161 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     5221 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     5281 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     5341 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     5401 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     5461 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     5521 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     5581 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     5641 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     5701 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     5761 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     5821 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     5881 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     5941 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     6001 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     6061 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     6121 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     6181 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     6241 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     6301 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     6361 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     6421 aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa
     6481 gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg caacatggta
     6541 acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg
     6601 gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt
     6661 ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac
     6721 ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     6781 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     6841 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     6901 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
     6961 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
     7021 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
     7081 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
     7141 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
     7201 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
     7261 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
     7321 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
     7381 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga
     7441 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
     7501 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
     7561 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc
     7621 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag
     7681 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg
     7741 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag
     7801 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat
     7861 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg
     7921 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac
     7981 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat
     8041 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc
     8101 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga
     8161 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggaccctt gcgccttttc
     8221 caaggcagcc ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc cgggaaacgc
     8281 agcggcgccg accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt cacccggatc
     8341 ttcgccgcta cccttgtggg ccccccggcg acgcttcctg ctccgcccct aagtcgggaa
     8401 ggttccttgc ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg tctcactagt
     8461 accctcgcag acggacagcg ccagggagca atggcagcgc gccgaccgcg atgggctgtg
     8521 gccaatagcg gctgctcagc agggcgcgcc gagagcagcg gccgggaagg ggcggtgcgg
     8581 gaggcggggt gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg ttccgcattc
     8641 tgcaagcctc cggagcgcac gtcggcagtc ggctccctcg ttgaccgaat caccgacctc
     8701 tctccccagg gggtaccacc atggccaagc ctttgtctca agaagaatcc accctcattg
     8761 aaagagcaac ggctacaatc aacagcatcc ccatctctga agactacagc gtcgccagcg
     8821 cagctctctc tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat tttactgggg
     8881 gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc tgcggcagct ggcaacctga
     8941 cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt gagcccctgc ggacggtgcc
     9001 gacaggtgct tctcgatctg catcctggga tcaaagccat agtgaaggac agtgatggac
     9061 agccgacggc agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg gagggcctgc
     9121 agctgcagta gtaagaattc tagatcttga gacaaatggc agtattcatc cacaatttta
     9181 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     9241 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
     9301 attacaggga cagcagagat ccactttggc gccggctcga gggggcccgg g
//
