LOCUS       sequence-334577-       11044 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpresses KCNH8.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11044)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 11044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_KCNH8_OE_1
FEATURES             Location/Qualifiers
     source          1..11044
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        146..449
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        450..653
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     604..624
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    676..700
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4023..4047)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4049..4090
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4132..4720
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4185..4205)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4603..4614)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4792..5025
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5103..5224
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5140..5159)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5194..5213
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5264..5399
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5419..5438)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5420..5438)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5448..5465)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5448..5464)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5457..5479)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5606..6061
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5693..5712)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5903..5924
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6087..6191
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6192..6260,6261..7052)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6410..6429)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7223..7811
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7712..7731
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7965..7982
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8099..8120
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8135..8140,8141..8158,8159..8165)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8173..8189
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8178..8200
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8197..8213
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8197..8213
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8232..8252
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8234..8252
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8280..8506
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8507..8687
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8734..8859
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9352..9585
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9770..9814
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9989..10489
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     10408..10426
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             10501..10896
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    join(10956..11044,1..29)
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccac
       61 tttggcgccg gctcgagggg gcccggggtt attaatagta atcaattacg gggtcattag
      121 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      181 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      241 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      301 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      361 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      421 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      481 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      541 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      601 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
      661 taactgtcgg gatcaacaag tttgtacaaa aaagcaggct atgccggtta tgaaaggatt
      721 actggcgccg caaaacacct tcctggacac catcgccacc cgttttgacg gaacacatag
      781 caacttcatc cttgccaatg cccaggtggc taagggtttc cccatagtct actgttccga
      841 tggcttctgc gagcttgctg gatttgcccg aactgaagtc atgcagaaga gttgtagctg
      901 caagttctta tttggggttg aaaccaatga gcaactgatg cttcaaatag aaaagtcact
      961 ggaggagaaa acagaattca aaggagaaat tatgttctac aagaaaaacg ggtctccatt
     1021 ttggtgccta ctggatattg ttcccataaa gaatgaaaaa ggagatgtag tactttttct
     1081 ggcctcgttc aaagatataa cagatacaaa agtgaagatt actccagaag ataaaaaaga
     1141 agacaaagtc aaaggaagat caagagcagg gacccacttt gactcagccc ggagacggag
     1201 tcgagcagtc ctttatcaca tctctgggca cctgcaaaga agagaaaaga acaaattgaa
     1261 aataaataac aatgtttttg tagataaacc agcatttccg gagtataaag tttctgatgc
     1321 aaaaaagtcc aaattcatac ttctgcattt tagcactttt aaagctggct gggactggct
     1381 tattttgttg gcaacgtttt atgttgctgt gactgtacct tacaacgttt gctttattgg
     1441 caatgacgac ctgtccacaa ctcggagcac aaccgtcagt gacattgcag tggagattct
     1501 ttttattata gatattattt taaatttccg aacaacttat gtcagcaagt ctggccaagt
     1561 tatctttgaa gcaagatcaa tttgcatcca ctatgtcaca acctggttca tcattgattt
     1621 aatcgctgcc ctgccttttg atcttctgta tgctttcaac gtcacagtgg tgtctctcgt
     1681 gcatcttcta aagacagtgc gcctcttgcg tcttttgcgt ctgctgcaga agttagaccg
     1741 ctattcccaa cacagtacta tcgtcctgac tctgctcatg tccatgtttg cactccttgc
     1801 acactggatg gcgtgtatct ggtacgtcat tggaaaaatg gagagggaag acaacagcct
     1861 tctgaagtgg gaagttggtt ggcttcatga gttgggaaag agactggaat ctccatacta
     1921 tggcaacaat accttggggg gcccgtcgat ccgaagtgcc tatattgccg ctctgtactt
     1981 cacgctgagc agcctcacca gcgtgggttt tgggaacgtc tctgctaata cagatgcaga
     2041 aaagatcttc tccatctgca ccatgctgat tggtgccttg atgcacgcct tggtgtttgg
     2101 aaacgtgaca gcaatcatac agaggatgta ctccagatgg tccctctatc acactagaac
     2161 taaggatctg aaagatttca tccgtgtcca tcacttgccc caacaactca agcagaggat
     2221 gctcgaatat tttcaaacaa cctggtcagt caacaatgga atagattcaa atgagctttt
     2281 gaaagacttt ccagatgaac tgcgttctga catcactatg cacttgaaca aggagatctt
     2341 acagttgtcc ctttttgaat gtgccagccg gggctgcctc aggtctctgt ctctacacat
     2401 caaaacctct ttctgtgctc cgggggagta tctgctgcgt caaggggatg ctttgcaggc
     2461 catctacttt gtatgctcgg gctccatgga agttcttaaa gacagcatgg tgctggctat
     2521 tcttgggaaa ggggatttaa ttggagcaaa tctatcaatt aaggaccaag tgatcaagac
     2581 caatgcagat gtaaaggctt taacctactg tgatctccag tgtatcatcc tcaaaggact
     2641 ctttgaagtg ctagaccttt acccagaata tgctcacaaa ttcgtggaag acattcagca
     2701 tgacctcaca tacaacctcc gagaaggtca tgagagtgat gtgatatcaa gactatcaaa
     2761 caaatctatg gtctcacagt cagagcccaa gggaaatggc aacatcaaca agcgactccc
     2821 atccattgtg gaagatgagg aagaggagga ggagggggag gaagaggagg cagtctccct
     2881 ctctcccatc tgcacaaggg gatcttcttc gcgcaacaag aaggttggaa gcaataaagc
     2941 ctacctgggc ttaagcttaa agcaactggc ctcgggaacg gtgccctttc actcgcctat
     3001 cagagtctcc aggtcaaatt cccccaaaac caagcaggaa attgaccccc ccaaccataa
     3061 taaaaggaaa gagaagaact tgaaattgca actttcaact ttgaataatg ctggaccccc
     3121 agacctcagt ccaaggattg ttgatggaat tgaagatgga aacagcagtg aagaaagtca
     3181 gacttttgat tttggctctg aacgaatcag atcagagccc agaatttctc ctcctcttgg
     3241 agatccagag attggagctg ctgttctctt catcaaagca gaggagacca agcagcagat
     3301 aaacaaactc aacagtgagg taacaacatt gactcaggaa gtttctcagt tgggtaaaga
     3361 catgagaaat gtgatccagc ttctggaaaa cgttctgtca cctcagcagc catcacggtt
     3421 ttgctctttg cacagcacct ctgtgtgtcc ctccagggag agcttacaga ccagaacgag
     3481 ctggagtgca caccagcctt gcctacactt gcaaacaggc ggggctgctt atacccaagc
     3541 acaactttgt agcagtaata tcacctcaga catttggagt gtggatccct cctctgtggg
     3601 gagcagcccc caacgaactg gagctcatga gcaaaatcct gcagacagtg aactttatca
     3661 ttctccaagc cttgattatt caccttccca ctaccaggtt gtccaagaag gtcatttgca
     3721 atttttaagg tgcatctctc cacattcaga ttctacgttg acgcctctgc agtccatttc
     3781 agcaactctc tcatcttctg tctgctcctc ttcggaaaca tctttgcacc tagttctccc
     3841 aagcagatca gaggagggca gcttcagtca gggaactgtg agttccttca gtctggaaaa
     3901 cttaccagga tcttggaacc aggaaggaat ggcatcagct tctacaaaac ctttggagaa
     3961 ccttccactg gaagttgtca caagcacagc agaagtgaaa gataacaaag ccataaatgt
     4021 atacccagct ttcttgtaca aagtggttgg taagcctatc cctaaccctc tcctcggtct
     4081 cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga caatcaacct
     4141 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     4201 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     4261 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     4321 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     4381 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     4441 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     4501 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     4561 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     4621 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     4681 cctcagacga gtcggatctc cctttgggcc gcctccccgc gtcgacttta agaccaatga
     4741 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     4801 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     4861 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     4921 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     4981 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt
     5041 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     5101 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     5161 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     5221 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc
     5281 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     5341 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     5401 agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
     5461 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     5521 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     5581 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     5641 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     5701 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     5761 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     5821 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     5881 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     5941 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     6001 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     6061 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     6121 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     6181 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     6241 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     6301 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     6361 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     6421 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     6481 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     6541 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     6601 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     6661 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     6721 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     6781 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     6841 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     6901 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     6961 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     7021 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     7081 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     7141 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     7201 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     7261 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     7321 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     7381 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     7441 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     7501 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     7561 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     7621 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     7681 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     7741 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     7801 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     7861 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     7921 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     7981 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     8041 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     8101 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     8161 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     8221 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     8281 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     8341 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     8401 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     8461 gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt
     8521 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     8581 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     8641 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     8701 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     8761 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     8821 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     8881 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     8941 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     9001 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     9061 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     9121 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     9181 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     9241 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     9301 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     9361 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     9421 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     9481 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     9541 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     9601 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     9661 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     9721 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     9781 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     9841 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     9901 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     9961 gacccacctc ccaaccccga ggggaccctt gcgccttttc caaggcagcc ctgggtttgc
    10021 gcagggacgc ggctgctctg ggcgtggttc cgggaaacgc agcggcgccg accctgggtc
    10081 tcgcacattc ttcacgtccg ttcgcagcgt cacccggatc ttcgccgcta cccttgtggg
    10141 ccccccggcg acgcttcctg ctccgcccct aagtcgggaa ggttccttgc ggttcgcggc
    10201 gtgccggacg tgacaaacgg aagccgcacg tctcactagt accctcgcag acggacagcg
    10261 ccagggagca atggcagcgc gccgaccgcg atgggctgtg gccaatagcg gctgctcagc
    10321 agggcgcgcc gagagcagcg gccgggaagg ggcggtgcgg gaggcggggt gtggggcggt
    10381 agtgtgggcc ctgttcctgc ccgcgcggtg ttccgcattc tgcaagcctc cggagcgcac
    10441 gtcggcagtc ggctccctcg ttgaccgaat caccgacctc tctccccagg gggtaccacc
    10501 atggccaagc ctttgtctca agaagaatcc accctcattg aaagagcaac ggctacaatc
    10561 aacagcatcc ccatctctga agactacagc gtcgccagcg cagctctctc tagcgacggc
    10621 cgcatcttca ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc agaactcgtg
    10681 gtgctgggca ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga
    10741 aatgagaaca ggggcatctt gagcccctgc ggacggtgcc gacaggtgct tctcgatctg
    10801 catcctggga tcaaagccat agtgaaggac agtgatggac agccgacggc agttgggatt
    10861 cgtgaattgc tgccctctgg ttatgtgtgg gagggcctgc agctgcagta gtaagaattc
    10921 tagatcttga gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
    10981 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
    11041 ttac
//
