LOCUS       sequence-334584-       11292 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpresses GRID1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11292)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 11292)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_GRID1_OE_1
FEATURES             Location/Qualifiers
     source          1..11292
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(640..1431,1432..1500))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1263..1282
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1501..1605)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1631..2086)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1768..1789)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1980..1999
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2228..2244
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2254..2272
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      2293..2428
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    2468..2589
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2479..2498)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2533..2552
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             2667..2900
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    2972..3560
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             3078..3089
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3487..3507
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3602..3643)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    3645..3669
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4060..4159)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4175..4191)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      4255..4341
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4433..4460
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     rep_origin      complement(4790..5378)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5471..6262)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     6693..6709
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6714..6732
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    6750..6849
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(7240..7264)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(7287..7490)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(7316..7336)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        7491..7794
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    7911..8028
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(8088..8483)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     promoter        complement(8495..8995)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8558..8576)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             complement(9170..9214)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9399..9632
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    10125..10250
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             10297..10477
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(10478..10704)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(10732..10752)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(10732..10750)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10771..10787)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10784..10806)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10795..10811
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10819..10825,10826..10843,10844..10849))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10864..10885
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(join(11173..11292,1..469))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
       61 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      121 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      181 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      241 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      301 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      361 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      421 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      481 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      541 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
      601 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
      661 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
      721 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
      781 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
      841 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
      901 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
      961 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     1021 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     1081 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     1141 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     1201 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     1261 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     1321 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     1381 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     1441 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     1501 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     1561 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     1621 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     1681 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
     1741 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     1801 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     1861 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
     1921 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     1981 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
     2041 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
     2101 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
     2161 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
     2221 cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg
     2281 ggtacgtccc taggcctcca aaaaagcctc ctcactactt ctggaatagc tcagaggccg
     2341 aggcggcctc ggcctctgca taaataaaaa aaattagtca gccatggggc ggagaatggg
     2401 cggaactggg cggagttagg ggcgggatgg gcggagttag gggcgggata gctagagcca
     2461 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     2521 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     2581 aaacaagttc ctctcactct ctgatattca tttctttgca agttataaat actgaataat
     2641 aagatgacat gaactactat acgtactgct agagattttc cacactgact aaaagggtct
     2701 gagggatctc tagttaccag agtcacacaa cagacgggca cacactactt gaagcactca
     2761 aggcaagctt tattgaggct taagcagtgg gttccctagt tagccagaga gctcccaggc
     2821 tcagatctgg tctaaccaga gagacccagt acaagcaaaa agcagatctt gtcttcgttg
     2881 ggagtgaatt agcccttcca gtcccccctt ttcttttaaa aagtggctaa gatctacagc
     2941 tgccttgtaa gtcattggtc ttaaagtcga cgcggggagg cggcccaaag ggagatccga
     3001 ctcgtctgag ggcgaaggcg aagacgcgga agaggccgca gagccggcag caggccgcgg
     3061 gaaggaaggt ccgctggatt gagggccgaa gggacgtagc agaaggacgt cccgcgcaga
     3121 atccaggtgg caacacaggc gagcagccaa ggaaaggacg atgatttccc cgacaacacc
     3181 acggaattgt cagtgcccaa cagccgagcc cctgtccagc agcgggcaag gcaggcggcg
     3241 atgagttccg ccgtggcaat agggaggggg aaagcgaaag tcccggaaag gagctgacag
     3301 gtggtggcaa tgccccaacc agtgggggtt gcgtcagcaa acacagtgca caccacgcca
     3361 cgttgcctga caacgggcca caactcctca taaagagaca gcaaccagga tttatacaag
     3421 gaggagaaaa tgaaagccat acgggaagca atagcatgat acaaaggcat taaagcagcg
     3481 tatccacata gcgtaaaagg agcaacatag ttaagaatac cagtcaatct ttcacaaatt
     3541 ttgtaatcca gaggttgatt gtcgacttaa cgcgccaccg gttagcgcta gctcattact
     3601 acgtagaatc gagaccgagg agagggttag ggataggctt accaaccact ttgtacaaga
     3661 aagttgggca gatggaggtc ccgtgggagg tgtccagagc ctctggaagg acgcctccag
     3721 gcacgggctg gaaggacatg gggatggggg tcttcaccgg gctctgccgg aacagccccc
     3781 cgttgggtga cctgtgtttg cactgcatgg agggcatggt cgccgagctg ctcagcggca
     3841 gcggcaggtt gctgctgggc cctgatgaga gtgtccggct ggtgccatgg ctgctctgct
     3901 ctggcagaaa ggtgctgacc gagagctggg tgttctggta ctcccgtgtc ggctccaagg
     3961 tctgggtggg agccaggccc cccatctcca gggccgagag ctcaatcgac gctggggaaa
     4021 tctgcttgtg agcaatgtct tcatccatga ggctgttcat gccaactttt ttgtacaaag
     4081 ttggcattat aaaaaagcat tgctcatcaa tttgttgcaa cgaacaggtc actatcagtc
     4141 aaaataaaat cattatttgg ggcccgagct taagactggc cgtcgtttta caacgtcgtg
     4201 actgggaaaa catccatgct agcgttaacg cgagagtagg gaactgccag gcatcaaata
     4261 aaacgaaagg ctcagtcgga agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     4321 gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgtgaa gcaacggccc
     4381 ggagggtggc gggcaggacg cccgccataa actgccaggc atcaaactaa gcagaaggcc
     4441 atcctgacgg atggcctttt tgcgtttcta caaactcttc ctggctagcg gtacgcgtat
     4501 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     4561 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     4621 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     4681 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     4741 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     4801 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     4861 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     4921 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     4981 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     5041 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     5101 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     5161 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     5221 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     5281 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     5341 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     5401 cggggtctga cgctcagtgg aacgacgcgt aactcacgtt aagggatttt ggtcatgggt
     5461 ggctcgaggg ttatttgccg actaccttgg tgatctcgcc tttcacgtag tggacaaatt
     5521 cttccaactg atctgcgcgc gaggccaagc gatcttcttc ttgtccaaga taagcctgtc
     5581 tagcttcaag tatgacgggc tgatactggg ccggcaggcg ctccattgcc cagtcggcag
     5641 cgacatcctt cggcgcgatt ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa
     5701 gcactacatt tcgctcatcg ccagcccagt cgggcggcga gttccatagc gttaaggttt
     5761 catttagcgc ctcaaataga tcctgttcag gaaccggatc aaagagttcc tccgccgctg
     5821 gacctaccaa ggcaacgcta tgttctcttg cttttgtcag caagatagcc agatcaatgt
     5881 cgatcgtggc tggctcgaag atacctgcaa gaatgtcatt gcgctgccat tctccaaatt
     5941 gcagttcgcg cttagctgga taacgccacg gaatgatgtc gtcgtgcaca acaatggtga
     6001 cttctacagc gcggagaatc tcgctctctc caggggaagc cgaagtttcc aaaaggtcgt
     6061 tgatcaaagc tcgccgcgtt gtttcatcaa gccttacggt caccgtaacc agcaaatcaa
     6121 tatcactgtg tggcttcagg ccgccatcca ctgcggagcc gtacaaatgt acggccagca
     6181 acgtcggttc gagatggcgc tcgatgacgc caactacctc tgatagttga gtcgatactt
     6241 cggcgatcac cgcttccctc ataatgttta actttgtttt agggcgactg ccctgctgcg
     6301 taacatcgtt gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct
     6361 tggatgcccg aggcatagac tgtaccccaa aaaaacagtc ataacaagcc atgaaaaccg
     6421 ccactgcgcc gttaccaccg ctgcgttcgg tcaaggttct ggaccagttg cgtgagcgca
     6481 tacgctactt gcattacagc ttacgaaccg aacaggctta tgtccactgg gttcgtgcct
     6541 tcatccgttt ccacggtgtg cgtcacccgg caaccttggg tagcagcgaa gtcgaggcat
     6601 ttctgtcctg gctggtctag aggaggcatg atgatatatt tttatcttgt gcaatgtaac
     6661 atcagagatt ttgagacacg ggccagagct gccaggaaac agctatgacc atgtaatacg
     6721 actcactata ggggatatca gctggatggc aaataatgat tttattttga ctgatagtga
     6781 cctgttcgtt gcaacaaatt gataagcaat gctttcttat aatgccaact ttgtacaaga
     6841 aagttgggca gatggaggtc ccgtgggagg tgtccagagc ctctggaagg acgcctccag
     6901 gcacgggctg gaaggacatg gggatggggg tcttcaccgg gctctgccgg aacagccccc
     6961 cgttgggtga cctgtgtttg cactgcatgg agggcatggt cgccgagctg ctcagcggca
     7021 gcggcaggtt gctgctgggc cctgatgaga gtgtccggct ggtgccatgg ctgctctgct
     7081 ctggcagaaa ggtgctgacc gagagctggg tgttctggta ctcccgtgtc ggctccaagg
     7141 tctgggtggg agccaggccc cccatctcca gggccgagag ctcaatcgac gctggggaaa
     7201 tctgcttgtg agcaatgtct tcatccatga ggctgttcat gccaactttt ttgtacaaac
     7261 ttgttgatcc cgacagttag ccagagagct ctgcttatat agacctccca ccgtacacgc
     7321 ctaccgccca tttgcgtcaa tggggcggag ttgttacgac attttggaaa gtcccgttga
     7381 ttttggtgcc aaaacaaact cccattgacg tcaatggggt ggagacttgg aaatccccgt
     7441 gagtcaaacc gctatccacg cccattgatg tactgccaaa accgcatcac catggtaata
     7501 gcgatgacta atacgtagat gtactgccaa gtaggaaagt cccataaggt catgtactgg
     7561 gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt acttggcata
     7621 tgatacactt gatgtactgc caagtgggca gtttaccgta aatactccac ccattgacgt
     7681 caatggaaag tccctattgg cgttactatg ggaacatacg tcattattga cgtcaatggg
     7741 cgggggtcgt tgggcggtca gccaggcggg ccatttaccg taagttatgt aacgcggaac
     7801 tccatatatg ggctatgaac taatgacccc gtaattgatt actattaata accccgggcc
     7861 ccctcgagcc ggcgccaaag tggatctctg ctgtccctgt aataaacccg aaaattttga
     7921 atttttgtaa tttgtttttg taattcttta gtttgtatgt ctgttgctat tatgtctact
     7981 attctttccc ctgcactgta ccccccaatc cccccttttc ttttaaaatt gtggatgaat
     8041 actgccattt gtctcaagat ctagaattct tactactgca gctgcaggcc ctcccacaca
     8101 taaccagagg gcagcaattc acgaatccca actgccgtcg gctgtccatc actgtccttc
     8161 actatggctt tgatcccagg atgcagatcg agaagcacct gtcggcaccg tccgcagggg
     8221 ctcaagatgc ccctgttctc atttccgatc gcgacgatac aagtcaggtt gccagctgcc
     8281 gcagcagcag cagtgcccag caccacgagt tctgcacaag gtcccccagt aaaatgatat
     8341 acattgacac cagtgaagat gcggccgtcg ctagagagag ctgcgctggc gacgctgtag
     8401 tcttcagaga tggggatgct gttgattgta gccgttgctc tttcaatgag ggtggattct
     8461 tcttgagaca aaggcttggc catggtggta ccccctgggg agagaggtcg gtgattcggt
     8521 caacgaggga gccgactgcc gacgtgcgct ccggaggctt gcagaatgcg gaacaccgcg
     8581 cgggcaggaa cagggcccac actaccgccc cacaccccgc ctcccgcacc gccccttccc
     8641 ggccgctgct ctcggcgcgc cctgctgagc agccgctatt ggccacagcc catcgcggtc
     8701 ggcgcgctgc cattgctccc tggcgctgtc cgtctgcgag ggtactagtg agacgtgcgg
     8761 cttccgtttg tcacgtccgg cacgccgcga accgcaagga accttcccga cttaggggcg
     8821 gagcaggaag cgtcgccggg gggcccacaa gggtagcggc gaagatccgg gtgacgctgc
     8881 gaacggacgt gaagaatgtg cgagacccag ggtcggcgcc gctgcgtttc ccggaaccac
     8941 gcccagagca gccgcgtccc tgcgcaaacc cagggctgcc ttggaaaagg cgcaagggtc
     9001 ccctcggggt tgggaggtgg gtctgaaacg ataatggtga atatccctgc ctaactctat
     9061 tcactataga aagtacagca aaaactattc ttaaacctac caagcctcct actatcatta
     9121 tgaataattt tatataccac agccaatttg ttatgttaaa ccaattccac aaacttgccc
     9181 atttatctaa ttccaataat tcttgttcat tcttttcttg ctggttttgc gattcttcaa
     9241 ttaaggagtg tattaagctt gtgtaattgt taatttctct gtcccactcc atccaggtcg
     9301 tgtgattcca aatctgttcc agagatttat tactccaact agcattccaa ggcacagcag
     9361 tggtgcaaat gagttttcca gagcaacccc aaatccccag gagctgttga tcctttaggt
     9421 atctttccac agccaggatt cttgcctgga gctgcttgat gccccagact gtgagttgca
     9481 acagatgctg ttgcgcctca atagccctca gcaaattgtt ctgctgctgc actataccag
     9541 acaataattg tctggcctgt accgtcagcg tcattgacgc tgcgcccata gtgcttcctg
     9601 ctgctcccaa gaacccaagg aacaaagctc ctattcccac tgctcttttt tctctctgca
     9661 ccactcttct ctttgccttg gtgggtgcta ctcctaatgg ttcaattttt actactttat
     9721 atttatataa ttcacttctc caattgtccc tcatatctcc tcctccaggt ctgaagatca
     9781 gcggccgctt gctgtgcggt ggtcttactt ttgttttgct cttcctctat cttgtctaaa
     9841 gcttccttgg tgtcttttat ctctatcctt tgatgcacac aatagagggt tgctactgta
     9901 ttatataatg atctaagttc ttctgatcct gtctgaaggg atggttgtag ctgtcccagt
     9961 atttgtctac agccttctga tgtttctaac aggccaggat taactgcgaa tcgttctagc
    10021 tccctgcttg cccatactat atgttttaat ttatattttt tctttccccc tggccttaac
    10081 cgaatttttt cccatcgcga tctaattctc ccccgcttaa tactgacgct ctcgcaccca
    10141 tctctctcct tctagcctcc gctagtcaaa atttttggcg tactcaccag tcgccgcccc
    10201 tcgcctcttg ccgtgcgcgc ttcagcaagc cgagtcctgc gtcgagagag ctcctctggt
    10261 ttccctttcg ctttcaagtc cctgttcggg cgccactgct agagattttc cacactgact
    10321 aaaagggtct gagggatctc tagttaccag agtcacacaa cagacgggca cacactactt
    10381 gaagcactca aggcaagctt tattgaggct taagcagtgg gttccctagt tagccagaga
    10441 gctcccaggc tcagatctgg tctaaccaga gagacccgtt tatgtatcga gctaggcact
    10501 taaatacaat atctctgcaa tgcggcaatt cagtggttcg tccaatccat gtcagacccg
    10561 tctgttgcct tcctaataag gcacgatcgt accaccttac ttccaccaat cggcatgcac
    10621 ggtgcttttt ctctccttgt aaggcatgtt gctaactcat cgttaccatg ttgcaagact
    10681 acaagagtat tgcataagac tacattaagc ttgcagctcc agcttttgtt ccctttagtg
    10741 agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
    10801 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
    10861 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
    10921 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
    10981 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
    11041 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
    11101 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
    11161 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
    11221 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
    11281 ctccctcgtg cg
//
