LOCUS       sequence-334610-       10472 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Non-integrating (episomal) expression of human OCT3/4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10472)
  AUTHORS   Okita K, Yamakawa T, Matsumura Y, Sato Y, Amano N, Watanabe A, 
            Goshima N, Yamanaka S
  TITLE     An Efficient Non-viral Method to Generate Integration-Free Human iPS
            Cells from Cord Blood and Peripheral Blood Cells.
  JOURNAL   Stem Cells. 2012 Nov 29. doi: 10.1002/stem.1293.
  PUBMED    23193063
REFERENCE   2  (bases 1 to 10472)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cells.
            2012 Nov 29. doi: 10.1002/stem.1293."
COMMENT     Sequence Label: pCE-hOCT3/4
FEATURES             Location/Qualifiers
     source          1..10472
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(83..102)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    148..203
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(202..221)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(564..580)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(564..580)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(577..599)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    588..604
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(612..618,619..636,637..642))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    657..678
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             1157..3082
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGXGGGXGXGGGXGGXGGXGXGGGXGXGGGXGGXGXGGGX
                     GGXGGXGXGGGXGXGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGXGAGGXGGXGAGGXGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     rep_origin      3384..5173
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(5714..5731)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5885..6473)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5965..5984)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6644..7435,7436..7504))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7267..7286
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7505..7609)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        7640..8019
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          8299..9315
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     9239..9261
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     9323..9342
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
ORIGIN
        1 aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg gccaatgccc
       61 tggctcacaa ataccactga gatctttttc cctctgccaa aaattatggg gacatcatga
      121 agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt
      181 gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac
      241 atcagaatga gtatttggtt tagagtttgg caacatatgc ccatatgctg gctgccatga
      301 acaaaggttg gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct
      361 tattccatag aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat
      421 ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc
      481 tctcctgact actcccagtc atagctgtcc ctcttctctt atggagatcc ctcgacctgc
      541 agcccaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
      601 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
      661 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
      721 tcgtgccagc ggatctcaat tctcatgttt gacagcttat catcgataag ctgatcctca
      781 caggccgcac ccagcttttc ttccgttgcc ccagtagcat ctctgtctgg tgaccttgaa
      841 gaggaagagg aggggtcccg agaatcccca tccctaccgt ccagcaaaaa gggggacgag
      901 gaatttgagg cctggcttga ggctcaggac gcaaatcttg aggatgttca gcgggagttt
      961 tccgggctgc gagtaattgg tgatgaggac gaggatggtt cggaggatgg ggaattttca
     1021 gacctggatc tgtctgacag cgaccatgaa ggggatgagg gtgggggggc tgttggaggg
     1081 ggcaggagtc tgcactccct gtattcactg agcgtcgtct aataaagatg tctattgatc
     1141 tcttttagtg tgaatcatgt ctgacgaggg gccaggtaca ggacctggaa atggcctagg
     1201 agagaaggga gacacatctg gaccagaagg ctccggcggc agtggacctc aaagaagagg
     1261 gggtgataac catggacgag gacggggaag aggacgagga cgaggaggcg gaagaccagg
     1321 agccccgggc ggctcaggat cagggccaag acatagagat ggtgtccgga gaccccaaaa
     1381 acgtccaagt tgcattggct gcaaagggac ccacggtgga acaggagcag gagcaggagc
     1441 gggaggggca ggagcaggag gggcaggagc aggaggaggg gcaggagcag gaggaggggc
     1501 aggaggggca ggaggggcag gaggggcagg agsaggagga ggggsaggag saggaggagg
     1561 ggsaggaggg gsaggagggg saggagsagg aggaggggsa ggagsaggag gaggggsagg
     1621 aggggsagga gsaggaggag gggsaggagg ggsaggaggg gsaggagsag gaggaggggs
     1681 aggagsagga ggaggggcag gaggggcagg agcaggagga ggggcaggag gggcaggagg
     1741 ggcaggagca ggaggagggg caggagcagg aggggcagga ggggcaggag gggcaggagc
     1801 aggaggggca ggagcaggag gaggggcagg aggggcagga ggggcaggag caggaggggc
     1861 aggagcagga ggggcaggag caggaggggc aggagcagga ggggcaggag gggcaggagc
     1921 aggaggggca ggaggggcag gagcaggagg ggcaggaggg gcaggagcag gaggaggggc
     1981 aggaggggca ggagcaggag gaggggcagg aggggsagga gcaggagggg saggaggggs
     2041 aggagcagga ggggsaggag gggcaggagc aggaggggca ggaggggcag gagcaggagg
     2101 aggggcagga gcaggagggg caggagcagg aggtggaggc cggggtcgag gaggcagtgg
     2161 aggccggggt cgaggaggta gtggaggccg gggtcgagga ggtagtggag gccgccgggg
     2221 tagaggacgt gaaagagcca gggggggaag tcgtgaaaga gccaggggga gaggtcgtgg
     2281 acgtggagaa aagaggccca ggagtcccag tagtcagtca tcatcatccg ggtctccacc
     2341 gcgcaggccc cctccaggta gaaggccatt tttccaccct gtaggggaag ccgattattt
     2401 tgaataccac caagaaggtg gcccagatgg tgagcctgac gtgcccccgg gagcgataga
     2461 gcagggcccc gcagatgacc caggagaagg cccaagcact ggaccccggg gtcagggtga
     2521 tggaggcagg cgcaaaaaag gagggtggtt tggaaagcat cgtggtcaag gaggttccaa
     2581 cccgaaattt gagaacattg cagaaggttt aagagctctc ctggctagga gtcacgtaga
     2641 aaggactacc gacgaaggaa cttgggtcgc cggtgtgttc gtatatggag gtagtaagac
     2701 ctccctttac aacctaaggc gaggaactgc ccttgctatt ccacaatgtc gtcttacacc
     2761 attgagtcgt ctcccctttg gaatggcccc tggacccggc ccacaacctg gcccgctaag
     2821 ggagtccatt gtctgttatt tcatggtctt tttacaaact catatatttg ctgaggtttt
     2881 gaaggatgcg attaaggacc ttgttatgac aaagcccgct cctacctgca atatcagggt
     2941 gactgtgtgc agctttgacg atggagtaga tttgcctccc tggtttccac ctatggtgga
     3001 aggggctgcc gcggagggtg atgacggaga tgacggagat gaaggaggtg atggagatga
     3061 gggtgaggaa gggcaggagt gatgtaactt gttaggagac gccctcaatc gtattaaaag
     3121 ccgtgtattc ccccgcacta aagaataaat ccccagtaga catcatgcgt gctgttggtg
     3181 tatttctggc catctgtctt gtcaccattt tcgtcctccc aacatggggc aattgggcat
     3241 acccatgttg tcacgtcact cagctccgcg ctcaacacct tctcgcgttg gaaaacatta
     3301 gcgacattta cctggtgagc aatcagacat gcgacggctt tagcctggcc tccttaaatt
     3361 cacctaagaa tgggagcaac cagcaggaaa aggacaagca gcgaaaattc acgccccctt
     3421 gggaggtggc ggcatatgca aaggatagca ctcccactct actactgggt atcatatgct
     3481 gactgtatat gcatgaggat agcatatgct acccggatac agattaggat agcatatact
     3541 acccagatat agattaggat agcatatgct acccagatat agattaggat agcctatgct
     3601 acccagatat aaattaggat agcatatact acccagatat agattaggat agcatatgct
     3661 acccagatat agattaggat agcctatgct acccagatat agattaggat agcatatgct
     3721 acccagatat agattaggat agcatatgct atccagatat ttgggtagta tatgctaccc
     3781 agatataaat taggatagca tatactaccc taatctctat taggatagca tatgctaccc
     3841 ggatacagat taggatagca tatactaccc agatatagat taggatagca tatgctaccc
     3901 agatatagat taggatagcc tatgctaccc agatataaat taggatagca tatactaccc
     3961 agatatagat taggatagca tatgctaccc agatatagat taggatagcc tatgctaccc
     4021 agatatagat taggatagca tatgctatcc agatatttgg gtagtatatg ctacccatgg
     4081 caacattagc ccaccgtgct ctcagcgacc tcgtgaatat gaggaccaac aaccctgtgc
     4141 ttggcgctca ggcgcaagtg tgtgtaattt gtcctccaga tcgcagcaat cgcgccccta
     4201 tcttggcccg cccacctact tatgcaggta ttccccgggg tgccattagt ggttttgtgg
     4261 gcaagtggtt tgaccgcagt ggttagcggg gttacaatca gccaagttat tacaccctta
     4321 ttttacagtc caaaaccgca gggcggcgtg tgggggctga cgcgtgcccc cactccacaa
     4381 tttcaaaaaa aagagtggcc acttgtcttt gtttatgggc cccattggcg tggagccccg
     4441 tttaattttc gggggtgtta gagacaacca gtggagtccg ctgctgtcgg cgtccactct
     4501 ctttcccctt gttacaaata gagtgtaaca acatggttca cctgtcttgg tccctgcctg
     4561 ggacacatct taataacccc agtatcatat tgcactagga ttatgtgttg cccatagcca
     4621 taaattcgtg tgagatggac atccagtctt tacggcttgt ccccacccca tggatttcta
     4681 ttgttaaaga tattcagaat gtttcattcc tacactagta tttattgccc aaggggtttg
     4741 tgagggttat attggtgtca tagcacaatg ccaccactga accccccgtc caaattttat
     4801 tctgggggcg tcacctgaaa ccttgttttc gagcacctca catacacctt actgttcaca
     4861 actcagcagt tattctatta gctaaacgaa ggagaatgaa gaagcaggcg aagattcagg
     4921 agagttcact gcccgctcct tgatcttcag ccactgccct tgtgactaaa atggttcact
     4981 accctcgtgg aatcctgacc ccatgtaaat aaaaccgtga cagctcatgg ggtgggagat
     5041 atcgctgttc cttaggaccc ttttactaac cctaattcga tagcatatgc ttcccgttgg
     5101 gtaacatatg ctattgaatt agggttagtc tggatagtat atactactac ccgggaagca
     5161 tatgctaccc gtttagggtt aacaaggggg ccttataaac actattgcta atgccctctt
     5221 gagggtccgc ttatcggtag ctacacaggc ccctctgatt gacgttggtg tagcctcccg
     5281 tagtcttcct gggcccctgg gaggtacatg tcccccagca ttggtgtaag agcttcagcc
     5341 aagagttaca cataaaggca atgttgtgtt gcagtccaca gactgcaaag tctgctccag
     5401 gatgaaagcc actcagtgtt ggcaaatgtg cacatccatt tataaggatg tcaactacag
     5461 tcagagaacc cctttgtgtt tggtcccccc ccgtgtcaca tgtggaacag ggcccagttg
     5521 gcaagttgta ccaaccaact gaagggatta catgcactgc cccgcgaaga aggggcagag
     5581 atgtcgtagt caggtttagt tcgtccgggg cggggatcga tcctctagag tcgacgctag
     5641 cggatccgct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     5701 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     5761 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     5821 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     5881 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     5941 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     6001 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     6061 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     6121 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     6181 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     6241 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     6301 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     6361 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     6421 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     6481 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     6541 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     6601 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     6661 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     6721 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     6781 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     6841 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     6901 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     6961 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     7021 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     7081 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     7141 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     7201 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     7261 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     7321 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     7381 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     7441 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     7501 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     7561 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     7621 gaaaagtgcc acctgggtcg acattgatta ttgactagtt attaatagta atcaattacg
     7681 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     7741 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     7801 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     7861 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     7921 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     7981 tggcagtaca tctacgtatt agtcatcgct attaccatgg tcgaggtgag ccccacgttc
     8041 tgcttcactc tccccatctc ccccccctcc ccacccccaa ttttgtattt atttattttt
     8101 taattatttt gtgcagcgat gggggcgggg gggggggggg ggcgcgcgcc rggggggggg
     8161 gggsggggsg gggggsgggg sggggsgrgg sggrgaggtg cggcggcagc caatcagagc
     8221 ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc ctataaaaag
     8281 cgaagcgcgc ggcgggcggg agtcgctgcg cgctgccttc gccccgtgcc ccgctccgcc
     8341 gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca ggtgagcggg
     8401 cgggacggcc cttctcctcc gggctgtaat tagcgcttgg tttaatgacg gcttgtttct
     8461 tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg gccctttgtg cggggggagc
     8521 ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg ccgcgtgcgg ctccgcgctg
     8581 cccggcggct gtgagcgctg cgggcgcggc gcggggcttt gtgcgctccg cagtgtgcgc
     8641 gaggggagcg cggccggggg cggtgccccg cggtgcgggg ggggctgcga ggggaacaaa
     8701 ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc gtcggtcggg
     8761 ctgcaacccc ccctgcaccc ccctccccga gttgctgagc acggcccggc ttcgggtgcg
     8821 gggctccgta cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg
     8881 ggggtgccgg gcggggcggg gccgcctcgg gccggggagg gctcggggga ggggcgcggc
     8941 ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg
     9001 taatcgtgcg agagggcgca gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct
     9061 gggaggcgcc gccgcacccc ctctagcggg cgcggggcga agcggtgcgg cgccggcagg
     9121 aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc gccgtcccct tctccctctc
     9181 cagcctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg cagggcgggg
     9241 ttcggcttct ggcgtgtgac cggcggctct agagcctctg ctaaccatgt tcatgccttc
     9301 ttctttttcc tacagctcct gggcaacgtg ctggttattg tgctgtctca tcattttggc
     9361 aaagaattcg cccttcacca tggcgggaca cctggcttcg gatttcgcct tctcgccccc
     9421 tccaggtggt ggaggtgatg ggccaggggg gccggagccg ggctgggttg atcctcggac
     9481 ctggctaagc ttccaaggcc ctcctggagg gccaggaatc gggccggggg ttgggccagg
     9541 ctctgaggtg tgggggattc ccccatgccc cccgccgtat gagttctgtg gggggatggc
     9601 gtactgtggg ccccaggttg gagtggggct agtgccccaa ggcggcttgg agacctctca
     9661 gcctgagggc gaagcaggag tcggggtgga gagcaactcc gatggggcct ccccggagcc
     9721 ctgcaccgtc acccctggtg ccgtgaagct ggagaaggag aagctggagc aaaacccgga
     9781 ggagtcccag gacatcaaag ctctgcagaa agaactcgag caatttgcca agctcctgaa
     9841 gcagaagagg atcaccctgg gatatacaca ggccgatgtg gggctcaccc tgggggttct
     9901 atttgggaag gtattcagcc aaacgaccat ctgccgcttt gaggctctgc agcttagctt
     9961 caagaacatg tgtaagctgc ggcccttgct gcagaagtgg gtggaggaag ctgacaacaa
    10021 tgaaaatctt caggagatat gcaaagcaga aaccctcgtg caggcccgaa agagaaagcg
    10081 aaccagtatc gagaaccgag tgagaggcaa cctggagaat ttgttcctgc agtgcccgaa
    10141 acccacactg cagcagatca gccacatcgc ccagcagctt gggctcgaga aggatgtggt
    10201 ccgagtgtgg ttctgtaacc ggcgccagaa gggcaagcga tcaagcagcg actatgcaca
    10261 acgagaggat tttgaggctg ctgggtctcc tttctcaggg ggaccagtgt cctttcctct
    10321 ggccccaggg ccccattttg gtaccccagg ctatgggagc cctcacttca ctgcactgta
    10381 ctcctcggtc cctttccctg agggggaagc ctttccccct gtctctgtca ccactctggg
    10441 ctctcccatg cattcaaact gaggtaaggg cg
//
