LOCUS       sequence-334620-        3104 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor DNA for TKIT knockin of Myc to GluA2 in mouse.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Fang H, Bygrave AM, Roth RH, Johnson RC, Huganir RL
  TITLE     An optimized CRISPR/Cas9 approach for precise genome editing in 
            neurons.
  JOURNAL   Elife. 2021 Mar 10;10. pii: 65202. doi: 10.7554/eLife.65202.
  PUBMED    33689678
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-03-10"; volume: "10. pii: 65202. doi"; pages: " 
            10.7554/eLife.65202"
COMMENT     Sequence Label: pMiniT:myc-GluA2 TKIT donor
FEATURES             Location/Qualifiers
     source          1..3104
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(106..123)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(277..865)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(357..376)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1036..1827,1828..1896))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1659..1678
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1897..2001)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2317..2346
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gtctaacatt ctgcgttcct ctttatcctg tctgaaccgg ctgcattaat gaatcggcca
       61 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
      121 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
      181 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
      241 ggccaggaac cgtaaaaagg ccgcattgct ggcgtttttc cataggctcc gcccccctga
      301 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
      361 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
      421 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
      481 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
      541 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
      601 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
      661 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
      721 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
      781 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
      841 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
      901 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
      961 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     1021 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     1081 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     1141 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     1201 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     1261 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     1321 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     1381 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     1441 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     1501 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     1561 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     1621 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     1681 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     1741 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     1801 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     1861 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     1921 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     1981 taaacaaata ggggttccgc gcacatttcc ccgactcgag aattccctct attgtgtcaa
     2041 aaggcatacc tgggagataa ggattcttct gccttcactt cgtggtttta gcagctcctt
     2101 gctaaatttc gacctcaaaa tgcagaggat ctaatttgct gaggaaaacg gtcaaagaag
     2161 gaaaaggagg aaagggaaac gaggggatat tttgtggatg ctctactttt cttggaaatg
     2221 caaaagatta tgcatatttc tgtcctcctt tctcctgttt tatggggact gatttttggt
     2281 gtctcttcta acactagtgg tggcggtggc tctagagaac aaaaactcat ctcagaagag
     2341 gatctcaagc ttggtggcgg tggctcgggc ggaggtgggt cgggtggcgg cggatcaggc
     2401 ggtggcggat ccagcataca gataggtagg taccttttgt gttatgcctt tgaattgtgc
     2461 ataatttggt ggtaatcttt gttcacagtg tgcaaattaa ttcatgtcat gtagacttat
     2521 gtcatacctt gactgcattc cagattgaaa ctacctagaa tatacatgat tttcttaggc
     2581 tgaagcaaaa agaaatgact atgccttttg acacaaccca ggtttagctg gtggctgttc
     2641 cgaattcgga tccctcgagc gatacagcga gtcgctcttt gtggaggtga cgattaccta
     2701 acaatcggtc gattcgtttg atgttatgtt ttgttctcgc tttggttggc aggttacggc
     2761 caagttcggt aagagtgaga gttttacagt caagtaatgc gtggcaagcc aacgttaagc
     2821 tgttgagtcg ttttaagtgt aattcggggc agaattggta aagagagtcg tgtaaaatat
     2881 cgagttcgca catcttgttg tctgattatt gatttttcgc gaaaccattt aatcatatga
     2941 caagatgtgt gtccacctta acttaatgat ttttaccaaa atcattaggg gattcatcag
     3001 cgctgagtgt gtaaattaat ttttatgccg cagcgggcca gcaattctcg tgaatcatcg
     3061 cttaaacggc ctgatttctg aatagctatg cgtaagcggg tgtt
//
