LOCUS       sequence-334628-        7741 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Reporter plasmid for B1H-dependent expression of the HIS3/GFP operon
            with a single hEGFP protospacer: PS1(upstream of promoter with 
            'CAGCG' PAM)-lac-HIS3 and GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7741)
  AUTHORS   Goldberg GW, Spencer JM, Giganti DO, Camellato BR, Agmon N, Ichikawa
            DM, Boeke JD, Noyes MB
  TITLE     Engineered dual selection for directed evolution of SpCas9 PAM 
            specificity.
  JOURNAL   Nat Commun. 2021 Jan 13;12(1):349. doi: 10.1038/s41467-020-20650-x.
  PUBMED    33441553
REFERENCE   2  (bases 1 to 7741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-01-13"; volume: "12(1):349. doi"; pages: " 
            10.1038/s41467-020-20650-x"
COMMENT     Sequence Label: pGG184
FEATURES             Location/Qualifiers
     source          1..7741
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1..87
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(625..1575)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      1623..1845
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(2337..2431)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2462..3256)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2573..2592)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3183..3202
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     terminator      complement(3405..3432)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(3448..3519)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(3534..4244)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3812..3831)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4081..4099
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     RBS             4250..4258
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(4311..5114)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     terminator      5220..5306
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5398..5425
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     5503..5521
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(5619..5624,5625..5642,5643..5649)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     5659..5681
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5678..5694
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             5690..6352
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     RBS             6400..6408
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             6414..7130
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             6414..7127
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      7145..7216
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      7232..7259
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(7274..7729)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7411..7432)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7623..7642
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
       61 gtgaacgctc tcctgagtag gacaaatccg ccgccctaga cctagggtac gggttttgct
      121 gcccgcaaac gggctgttct ggtgttgcta gtttgttatc agaatcgcag atccggcttc
      181 agccggtttg ccggctgaaa gcgctatttc ttccagaatt gccatgattt tttccccacg
      241 ggaggcgtca ctggctcccg tgttgtcggc agctttgatt cgataagcag catcgcctgt
      301 ttcaggctgt ctatgtgtga ctgttgagct gtaacaagtt gtctcaggtg ttcaatttca
      361 tgttctagtt gctttgtttt actggtttca cctgttctat taggtgttac atgctgttca
      421 tctgttacat tgtcgatctg ttcatggtga acagctttga atgcaccaaa aactcgtaaa
      481 agctctgatg tatctatctt ttttacaccg ttttcatctg tgcatatgga cagttttccc
      541 tttgatatgt aacggtgaac agttgttcta cttttgtttg ttagtcttga tgcttcactg
      601 atagatacaa gagccataag aacctcagat ccttccgtat ttagccagta tgttctctag
      661 tgtggttcgt tgtttttgcg tgagccatga gaacgaacca ttgagatcat acttactttg
      721 catgtcactc aaaaattttg cctcaaaact ggtgagctga atttttgcag ttaaagcatc
      781 gtgtagtgtt tttcttagtc cgttatgtag gtaggaatct gatgtaatgg ttgttggtat
      841 tttgtcacca ttcattttta tctggttgtt ctcaagttcg gttacgagat ccatttgtct
      901 atctagttca acttggaaaa tcaacgtatc agtcgggcgg cctcgcttat caaccaccaa
      961 tttcatattg ctgtaagtgt ttaaatcttt acttattggt ttcaaaaccc attggttaag
     1021 ccttttaaac tcatggtagt tattttcaag cattaacatg aacttaaatt catcaaggct
     1081 aatctctata tttgccttgt gagttttctt ttgtgttagt tcttttaata accactcata
     1141 aatcctcata gagtatttgt tttcaaaaga cttaacatgt tccagattat attttatgaa
     1201 tttttttaac tggaaaagat aaggcaatat ctcttcacta aaaactaatt ctaatttttc
     1261 gcttgagaac ttggcatagt ttgtccactg gaaaatctca aagcctttaa ccaaaggatt
     1321 cctgatttcc acagttctcg tcatcagctc tctggttgct ttagctaata caccataagc
     1381 attttcccta ctgatgttca tcatctgagc gtattggtta taagtgaacg ataccgtccg
     1441 ttctttcctt gtagggtttt caatcgtggg gttgagtagt gccacacagc ataaaattag
     1501 cttggtttca tgctccgtta agtcatagcg actaatcgct agttcatttg ctttgaaaac
     1561 aactaattca gacatacatc tcaattggtc taggtgattt taatcactat accaattgag
     1621 atgggctagt caatgataat tactagtcct tttcctttga gttgtgggta tctgtaaatt
     1681 ctgctagacc tttgctggaa aacttgtaaa ttctgctaga ccctctgtaa attccgctag
     1741 acctttgtgt gttttttttg tttatattca agtggttata atttatagaa taaagaaaga
     1801 ataaaaaaag ataaaaagaa tagatcccag ccctgtgtat aactcactac tttagtcagt
     1861 tccgcagtat tacaaaagga tgtcgcaaac gctgtttgct cctctacaaa acagacctta
     1921 aaaccctaaa ggcttaagta gcaccctcgc aagctcgggc aaatcgctga atattccttt
     1981 tgtctccgac catcaggcac ctgagtcgct gtctttttcg tgacattcag ttcgctgcgc
     2041 tcacggctct ggcagtgaat gggggtaaat ggcactacag gcgcctttta tggattcatg
     2101 caaggaaact acccataata caagaaaagc ccgtcacggg cttctcaggg cgttttatgg
     2161 cgggtctgct atgtggtgct atctgacttt ttgctgttca gcagttcctg ccctctgatt
     2221 ttccagtctg accacttcgg attatcccgt gacaggtcat tcagactggc taatgcaccc
     2281 agtaaggcag cggtatcatc aacaggctta cccgtcttac tgtccctagt gcttggattc
     2341 tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct
     2401 gaggtcatta ctggatctat caacaggagt ccaagcgagc tctcgaaccc cagagtcccg
     2461 ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga
     2521 taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca gcaatatcac
     2581 gggtagccaa cgctatgtcc tgatagcggt ccgccacacc cagccggcca cagtcgatga
     2641 atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg ccatgggtca
     2701 cgacgagatc ctcgccgtcg ggcatgcgcg ccttgagcct ggcgaacagt tcggctggcg
     2761 cgagcccctg atgctcttcg tccagatcat cctgatcgac aagaccggct tccatccgag
     2821 tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa
     2881 gcgtatgcag ccgccgcatt gcatcagcca tgatggatac tttctcggca ggagcaaggt
     2941 gagatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc cttcccgctt
     3001 cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc
     3061 gcgctgcctc gtcctgcagt tcattcaggg caccggacag gtcggtcttg acaaaaagaa
     3121 ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg attgtctgtt
     3181 gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct gcgtgcaatc
     3241 catcttgttc aatcatgcga aacgatcctc atcctgtctc ttgatcagat cttgatcccc
     3301 tgcgccatca gatccttggc ggcaagaaag ccatccagtt tactttgcag ggcttcccaa
     3361 ccttaccaga gggcgcccca gctggcaatt ccgacgtcta taaacgcaga aaggcccacc
     3421 cgaaggtgag ccagtgtgac tctagtagag agcgttcacc gacaaacaac agataaaacg
     3481 aaaggcccag tctttcgact gagcctttcg ttttatttga tgcctggcta tcattacttg
     3541 tacagctcgt ccatgcctcc tgtggagtgg cggccctcgg cgcgttcgta ctgttccacg
     3601 atggtgtagt cctcgttgtg ggaggtgatg tccaacttga tgttgacgtt gtaggcgccg
     3661 ggcagctgca cgggcttctt ggccttgtag gtggtcttga cctcagcgtc gtagtggccg
     3721 ccgtccttca gcttcagcct ctgcttgatc tcgcccttca gggcgccgtc ctcggggtac
     3781 atccgctcgg aggaggcctc ccagcccatg gtcttcttct gcattacggg gccgtcggag
     3841 gggaagttgg tgccgcgcag cttcaccttg tagatgaact cgccgtcctg cagggaggag
     3901 tcctgggtca cggtcaccac gccgccgtcc tcgaagttca tcacgcgctc ccacttgaag
     3961 ccctcgggga aggacagctt caagtagtcg gggatgtcgg cggggtgctt cacgtaggcc
     4021 ttggagccgt acatgaactg aggggacagg atgtcccagg cgaagggcag ggggccaccc
     4081 ttggtcacct tcagcttggc ggtctgggtg ccctcgtagg ggcggccctc gccctcgccc
     4141 tcgatctcga actcgtggcc gttcacggag ccctccatgt gcaccttgaa gcgcatgaac
     4201 tccttgatga tggccatgtt atcctcctcg cccttgctca ccatagctga cctccttatt
     4261 aaagttaaac aaaacgccgg tgctgcagct ttaaataatc ggtgtcacta ttagttttgc
     4321 tggccgcatc ttctcaaata tgcttcccag cctgcttttc tgtaacgttc accctctacc
     4381 ttagcatccc ttccctttgc aaatagtcct cttccaacaa taataatgtc agatcctgta
     4441 gagaccacat catccacggt tctatactgt tgacccaatg cgtctccctt gtcatctaaa
     4501 cccacaccgg gtgtcataat caaccaatcg taaccttcat ctcttccacc catgtctctt
     4561 tgagcaataa agccgataac aaaatctttg tcgctcttcg caatgtcaac agtaccctta
     4621 gtatattctc cagtagatag ggagcccttg catgacaatt ctgctaacat caaaaggcct
     4681 ctaggttcct ttgttacttc ttctgccgcc tgcttcaaac cgctaacaat acctgggccc
     4741 accacaccgt gtgcattcgt aatgtctgcc cattctgcta ttctgtatac acccgcagag
     4801 tactgcaatt tgactgtatt accaatgtca gcaaattttc tgtcttcgaa gagtaaaaaa
     4861 ttgtacttgg cggataatgc ctttagcggc ttaactgtgc cctccatgga aaaatcagtc
     4921 aagatatcca catgtgtttt tagtaaacaa attttgggac ctaatgcttc aactaactcc
     4981 agtaattcct tggtggtacg aacatccaat gaagcacaca agtttgtttg cttttcgtgc
     5041 atgatattaa atagcttggc agcaacagga ctaggatgag tagcagcacg ttccttatat
     5101 gtagctttcg acatagctgt ttcctgtgtg gctaccagta gcgtattact tgaggaacat
     5161 aagccgaaag gccataggat gtaaaaccgg tcgagcgagt gctttggcgc gccaggcatc
     5221 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     5281 tgaacgctct cctgagtagg acaaatccgc cgggagcgga tttgaacgtt gcgaagcaac
     5341 ggcccggagg gtggcgggca ggacgcccgc cataaactgc caggcatcaa attaagcaga
     5401 aggccatcct gacggatggc ctttttgcgt ttctacaaac tcttttgttt atttttctaa
     5461 atacattcaa atatgtatcc gctcatgaca ttaacctata aaaataggcg tatcacgagg
     5521 ccctttcgtc ttcaaacgcg tgtacaaaac cggcggccgc aacacgctga acttgtggcc
     5581 gtttacgtcg ctgtccagct cgaccaggat gggaattctt tacactttat gcttccggct
     5641 cgtatgttgt gtcgaccgag cggataacaa tttcacacag gaaacagcta tgacagagca
     5701 gaaagcccta gtaaagcgta ttacaaatga aaccaagatt cagattgcga tctctttaaa
     5761 gggtggtccc ctagcgatag agcactcgat cttcccagaa aaagaggcag aagcagtagc
     5821 agaacaggcc acacaatcgc aagtgattaa cgtccacaca ggtatagggt ttctggacca
     5881 tatgatacat gctctggcca agcattccgg ctggtcgcta atcgttgagt gcattggtga
     5941 cttacacata gacgaccatc acaccactga agactgcggg attgctctcg gtcaagcttt
     6001 taaagaggcc ctaggggccg tgcgtggagt aaaaaggttt ggatcaggat ttgcgccttt
     6061 ggatgaggca ctttccagag cggtggtaga tctttcgaac aggccgtacg cagttgtcga
     6121 acttggtttg caaagggaga aagtaggaga tctctcttgc gagatgatcc cgcattttct
     6181 tgaaagcttt gcagaggcta gcagaattac cctccacgtt gattgtctgc gaggcaagaa
     6241 tgatcatcac cgtagtgaga gtgcgttcaa ggctcttgcg gttgccataa gagaagccac
     6301 ctcgcccaat ggtaccaacg atgttccctc caccaaaggt gttcttatgt agtgacaccg
     6361 attatttaaa gctgcaggga tccttttgtt taactttaat aaggaggtca gctatgtcta
     6421 aaggtgaaga attattcact ggtgttgtcc caattttggt tgaattagat ggtgatgtta
     6481 atggtcacaa attttctgtc tccggtgaag gtgaaggtga tgctacttac ggtaaattga
     6541 ccttaaaatt tatttgtact actggtaaat tgccagttcc atggccaacc ttagtcacta
     6601 ctttaactta tggtgttcaa tgtttttcaa gatacccaga tcatatgaaa caacatgact
     6661 ttttcaagtc tgccatgcca gaaggttatg ttcaagaaag aactattttt ttcaaagatg
     6721 acggtaacta caagaccaga gctgaagtca agtttgaagg tgatacctta gttaatagaa
     6781 tcgaattaaa aggtattgat tttaaagaag atggtaacat tttaggtcac aaattggaat
     6841 acaactataa ctctcacaat gtttacatca tggctgacaa acaaaagaat ggtatcaaag
     6901 ttaacttcaa aattagacac aacattgaag atggttctgt tcaattagct gaccattatc
     6961 aacaaaatac tccaattggt gatggtccag tcttgttacc agacaaccat tacttatcca
     7021 ctcaatctgc cttatccaaa gatccaaacg aaaagagaga ccacatggtc ttgttagaat
     7081 ttgttactgc tgctggtatt acccatggta tggatgaatt gtacaaataa tagtgaccag
     7141 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     7201 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
     7261 ttatactcga gctaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
     7321 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     7381 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     7441 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     7501 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     7561 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     7621 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     7681 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc tctagaggca
     7741 t
//
