LOCUS       sequence-334632-       12463 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The lentiviral vector for AMPK FRET-biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12463)
  AUTHORS   Konagaya Y, Terai K, Hirao Y, Takakura K, Imajo M, Kamioka Y, 
            Sasaoka N, Kakizuka A, Sumiyama K, Asano T, Matsuda M
  TITLE     A Highly Sensitive FRET Biosensor for AMPK Exhibits Heterogeneous 
            AMPK Responses among Cells and Organs.
  JOURNAL   Cell Rep. 2017 Nov 28;21(9):2628-2638. doi: 
            10.1016/j.celrep.2017.10.113.
  PUBMED    29186696
REFERENCE   2  (bases 1 to 12463)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.10"; journalName: "Cell Rep"; date: 
            "2017-11-28- 28"; volume: "21"; issue: "9"; pages: "2628-2638"
COMMENT     Sequence Label: pCSIIbsr-4082NES(ForAMPK)
FEATURES             Location/Qualifiers
     source          1..12463
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1442..1675
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1860..1904
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2178..2295
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2351..3513
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2582..3504
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3461..3481
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3548..4258
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLLE
                     FLTAAGITEGMNELY"
     CDS             4694..5041
                     /codon_start=1
                     /product="long flexible linker consisting of SAGG repeats 
                     (Komatsu et al., 2011)"
                     /label=EV linker
                     /translation="SAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGS
                     AGGSAGGXGSAGGSAGGSXSAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGG
                     SAGGSAGGSAGG"
     CDS             join(5132..5134,5135..5137,5138..5650)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISGNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(5177..5198)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5438..5457)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             5855..5881
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     misc_feature    join(5895..6469,6470..6472,6473..6481)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6062..6079)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6289..6308
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6482..6880
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    6924..7512
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6977..6997)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7395..7406)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8031..8211
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8237..8254)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8243..8467
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8513..8941
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8600..8619)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8810..8831
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8950..8970)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8955..9284
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9135..9270
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9197..9216
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9332..9379
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9398..9772
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9902..10023
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9939..9958)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9993..10012
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10072..10088)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10072..10088)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10085..10107)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10096..10112
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10120..10126,10127..10144,10145..10150))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10165..10186
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10303..10320)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10474..11062)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10554..10573)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11233..12024,12025..12093))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11856..11875
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12094..12198)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12273..12292)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagggc cgctgatctt cagacctgga ggaggagata
     1321 tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag
     1381 gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa
     1441 taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa
     1501 tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt
     1561 tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc
     1621 agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga
     1681 tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga
     1741 gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa
     1801 ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa
     1861 agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca
     1921 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt
     1981 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat
     2041 tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag
     2101 aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga tctacaaatg
     2161 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2221 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2281 aaaattcaaa attttcgggt ttattacagg gacagcagaa attcactttg aattaattca
     2341 agcttcgtga ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga
     2401 gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa
     2461 ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta
     2521 tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca
     2581 ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt
     2641 gccttgaatt acttccacct ggctccagta cgtgattctt gatcccgagc tggagccagg
     2701 ggcgggcctt gcgctttagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg
     2761 cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat
     2821 aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat
     2881 agtcttgtaa atgcgggcca ggatctgcac actggtattt cggtttttgg ggccgcgggc
     2941 ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg
     3001 ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc
     3061 gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg
     3121 tgagcggaaa gatggccgct tcccggccct gctccagggg gctcaaaatg gaggacgcgg
     3181 cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaggggcctt tccgtcctca
     3241 gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc
     3301 tggagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt
     3361 ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc
     3421 ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt
     3481 caaagttttt ttcttccatt tcaggtgtcg tgaacacgct accggtctcg acgaattcgc
     3541 ccttagcatg tctaaaggtg aagaattatt cactggtgtt gtcccaattt tggttgaatt
     3601 agatggtgat gttaatggtc acaaattttc tgtctccggt gaaggtgaag gtgatgctac
     3661 gtacggtaaa ttgaccttaa aattactctg tactactggt aaattgccag ttccatggcc
     3721 aaccttagtc actactttag gttatggtgt tcaatgtttt gctagatacc cagatcatat
     3781 gaaacaacat gactttttca agtctgccat gccagaaggt tatgttcaag aaagaactat
     3841 ttttttcaaa gatgacggta actacaagac cagagctgaa gtcaagtttg aaggtgatac
     3901 cttagttaat agaatcgaat taaaaggtat tgattttaaa gaagatggta acattttagg
     3961 tcacaaattg gaatacaact ataactctca caatgtttac atcactgctg acaaacaaaa
     4021 gaatggtatc aaagctaact tcaaaattag acacaacatt gaagatggtg gtgttcaatt
     4081 agctgaccat tatcaacaaa atactccaat tggtgatggt ccagtcttgt taccagacaa
     4141 ccattactta tcctatcaat ctgccttatt caaagatcca aacgaaaaga gagaccacat
     4201 ggtcttgtta gaatttttga ctgctgctgg tattaccgag ggtatgaatg aattgtacct
     4261 cgagaagttt tctcaagaac agatcggcga aaacattgtg tgcagggtca tttgtaccac
     4321 gggtcaaatt cccatccgag atttgtcagc tgatatttca caagtgctta aggaaaaacg
     4381 atccataaag aaagtttgga catttggtag aaacccagcc tgtgactatc atttaggaaa
     4441 catttcaaga ctgtcaaata agcatttcca aatactacta ggagaagacg gtaacctttt
     4501 attgaatgac atttccacta atgggacctg gttaaatggg caaaaagtcg agaagaacag
     4561 caatcagtta ctgtcccaag gtgatgaaat aaccgttggt gtaggcgtgg aatcagatat
     4621 tttatctctg gtcattttca taaacgacaa atttaagcag tgcctggagc agaacaaagt
     4681 tgatcgcggt accagtgctg gtggtagtgc tggtggtagt gctggtggta gtgctggtgg
     4741 tagtgctggt ggttccggca gtgctggtgg tagtgctggt ggtagtacca gtgctggtgg
     4801 tagtgctggt ggtagtgctg gtggtagtgc tggtggtagt gctggtggtt syggcagtgc
     4861 tggtggtagt gctggtggta gtrccagtgc tggtggtagt gctggtggta gtgctggtgg
     4921 tagtgctggt ggtagtgctg gtggttccgg cagtgctggt ggtagtgctg gtggtagtac
     4981 cagtgctggt ggtagtgctg gtggtagtgc tggtggtagt gctggtggta gtgctggtgg
     5041 ttccggaggc agcggcgagg gcagcaccaa gatgaggagg gtggccaccc tggtggacct
     5101 gggcaccggc ggcagcgagc tgggcggccg catggtgagc aagggcgagg agctgttcac
     5161 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     5221 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     5281 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgtcct ggggcgtgca
     5341 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     5401 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     5461 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     5521 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca tcagcgggaa
     5581 cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca
     5641 caacatcgag gacggcggcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     5701 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccttaagcaa
     5761 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcttgaccg ccgccgggat
     5821 cactctcggc atggacgagc tgtctagact gcagctgcct cctctggaac gcctgactct
     5881 ggattaagga tccgcccctc tccctccccc ccccctaacg ttactggccg aagccgcttg
     5941 gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc
     6001 aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc
     6061 cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa
     6121 gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct
     6181 ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca
     6241 caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca
     6301 agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat
     6361 ctggggcctc ggtacacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc
     6421 cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata tggccacaac
     6481 catggccaag cctttgtctc aagaagaatc caccctcatt gaaagagcaa cggctacaat
     6541 caacagcatc cccatctctg aagactacag cgtcgccagc gcagctctct ctagcgacgg
     6601 ccgcatcttc actggtgtca atgtatatca ttttactggg ggaccttgtg cagaactcgt
     6661 ggtgctgggc actgctgctg ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg
     6721 aaatgagaac aggggcatct tgagcccctg cggacggtgc cgacaggtgc ttctcgatct
     6781 gcatcctggg atcaaagcca tagtgaagga cagtgatgga cagccgacgg cagttgggat
     6841 tcgtgaattg ctgccctctg gttatgtgtg ggagggctaa gtcgagcctc tagagttaac
     6901 atcgagggat caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga
     6961 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     7021 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     7081 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     7141 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     7201 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     7261 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc
     7321 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     7381 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     7441 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     7501 ccgcctcccc gcatcgatac cgtcgagacc tagaaaaaca tggagcaatc acaagtagca
     7561 atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg
     7621 gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     7681 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     7741 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tggcagaact
     7801 acacaccagg gccagggatc agatatccac tgacctttgg atggtgctac aagctagtac
     7861 cagttgagca agagaaggta gaagaagcca atgaaggaga gaacacccgc ttgttacacc
     7921 ctgtgagcct gcatgggatg gatgacccgg agagagaagt attagagtgg aggtttgaca
     7981 gccgcctagc atttcatcac atggcccgag agctgcatcc ggactgtact gggtctctct
     8041 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     8101 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     8161 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agggcccgtt
     8221 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     8281 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     8341 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     8401 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     8461 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     8521 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     8581 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     8641 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     8701 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     8761 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     8821 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     8881 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     8941 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     9001 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     9061 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     9121 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     9181 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
     9241 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
     9301 gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat
     9361 cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt
     9421 tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg
     9481 gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct
     9541 gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt
     9601 gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga
     9661 cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct
     9721 gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct
     9781 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
     9841 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
     9901 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     9961 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
    10021 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
    10081 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
    10141 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
    10201 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
    10261 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
    10321 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
    10381 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
    10441 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
    10501 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
    10561 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
    10621 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
    10681 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
    10741 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
    10801 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
    10861 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
    10921 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
    10981 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
    11041 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
    11101 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
    11161 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
    11221 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
    11281 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
    11341 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
    11401 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
    11461 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
    11521 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
    11581 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
    11641 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
    11701 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
    11761 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
    11821 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
    11881 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
    11941 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
    12001 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
    12061 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
    12121 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
    12181 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg
    12241 agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
    12301 agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt
    12361 taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg
    12421 cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gtt
//
