LOCUS       sequence-334646-        4680 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4680)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLCO2B1_STOP
FEATURES             Location/Qualifiers
     source          1..4680
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2237..2336)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2353..2372)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2354..2372)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2377..2393)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2377..2393)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2495..2514)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2506..3315
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2573..2592)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3462..4050
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3951..3970
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4204..4221
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4380..4407)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4499..4585)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4634..4656
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4648..4665
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4649..4665
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatggg cccaaggatc
      121 ggaccagcag gagaggtgcc tcaggtgcca gacaaggaga ccaaggccac aatgggaacc
      181 gagaacacac caggaggcaa ggcaagccca gaccctcagg atgtgcgccc ttccgtgttc
      241 cacaatatca agctgtttgt gctgtgccac agcctgctgc agctggccca gctgatgatc
      301 tctggctacc tgaagagctc catcagcacc gtggagaaga ggttcggcct gtctagccag
      361 acatctggcc tgctggccag ctttaacgaa gtgggcaata ccgccctgat cgtgttcgtg
      421 tcttactttg gcagccgcgt gcaccggcca agaatgatcg gctatggagc aatcctggtg
      481 gccctggcag gactgctgat gaccctgcct cacttcatct ctgagccata ccggtatgat
      541 aacacaagcc ctgaggacat gccacaggac ttcaaggcca gcctgtgcct gccaaccaca
      601 tccgccccag catctgcccc tagcaacgga aattgttcct cttataccga gacacagcac
      661 ctgtccgtgg tgggcatcat gttcgtggca cagaccctgc tgggagtggg aggcgtgccc
      721 atccagcctt tcggcatcag ctacatcgac gattttgccc acaactctaa tagcccactg
      781 tatctgggca tcctgttcgc agtgaccatg atgggaccag gactggcctt tggcctggga
      841 tctctgatgc tgcggctgta cgtggacatc aatcagatgc ctgagggcgg catcagcctg
      901 acaatcaagg atccaagatg ggtgggagca tggtggctgg gcttcctgat cgcagcagga
      961 gcagtggccc tggcagccat cccatatttc tttttcccaa aggagatgcc caaggagaag
     1021 cgggagctgc agtttcggag aaaggtgctg gcagtgaccg actccccagc aagaaagggc
     1081 aaggattccc catctaagca gtcccccggc gagtctacca agaagcagga cggcctggtg
     1141 cagatcgccc ctaacctgac agtgatccag ttcatcaagg tgtttccaag ggtgctgctg
     1201 cagaccctga ggcacccaat cttcctgctg gtggtgctga gccaggtgtg cctgagctcc
     1261 atggcagcag gaatggccat cttcctgccc aagtttctgg agagacagtt cagcatcaca
     1321 gcctcctacg ccaatctgct gatcggctgt ctgtcctttc catctgtgat cgtgggaatc
     1381 gtggtgggag gcgtgctggt gaagaggctg cacctgggac cagtgggatg cggcgccctg
     1441 tgcctgctgg gcatgctgct gtgcctgttt ttctccctgc ctctgttttt catcggctgt
     1501 tctagccacc agatcgccgg catcacccac cagacatctg cccacccagg actggagctg
     1561 agcccttcct gcatggaggc ctgcagctgt cccctggacg gcttcaaccc cgtgtgcgat
     1621 ccatccacca gagtggagta catcacacct tgccacgccg gctgttcctc ttgggtggtg
     1681 caggacgccc tggataactc ccaggtgttt tataccaatt gctcttgcgt ggtggagggc
     1741 aatcctgtgc tggccggcag ctgcgactcc acatgttctc acctggtggt gccattcctg
     1801 ctgctggtga gcctgggatc cgccctggca tgcctgaccc acacaccttc ctttatgctg
     1861 atcctgaggg gcgtgaagaa ggaggataag accctggccg tgggcatcca gttcatgttt
     1921 ctgcgcatcc tggcctggat gccatccccc gtgatccacg gctctgccat cgacaccaca
     1981 tgcgtgcact gggcactgag ctgcggcagg cgcgccgtgt gccgctacta taacaatgat
     2041 ctgctgagga accgcttcat cggcctgcag tttttcttta agaccggctc cgtgatctgt
     2101 tttgcactgg tgctggccgt gctgaggcag caggacaagg aggcaaggac aaaggagagc
     2161 agaagctccc cagcagtgga gcagcagctg ctggtgtccg gaccaggcaa gaagcctgag
     2221 gattctagag tgtaagaccc agctttcttg tacaaagttg gcattataag aaagcattgc
     2281 ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat
     2341 ccagctgata tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc
     2401 tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga
     2461 acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa
     2521 cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa
     2581 tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta tgggaagccc
     2641 gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat
     2701 gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt
     2761 atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa aacagcattc
     2821 caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc
     2881 ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt
     2941 cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat
     3001 gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca
     3061 ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac
     3121 gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag
     3181 gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt
     3241 tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc
     3301 gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag cattacgctg
     3361 acttgacggg acggcgcaag ctcatgacca aaatccctta acgtgagtta cgcgtcgttc
     3421 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     3481 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     3541 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     3601 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     3661 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     3721 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     3781 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     3841 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     3901 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     3961 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     4021 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     4081 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     4141 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     4201 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     4261 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     4321 agtgagcgca acgcaattaa tacgcgtacc gctagccagg aagagtttgt agaaacgcaa
     4381 aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc
     4441 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat
     4501 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     4561 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgcta
     4621 gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc
//
