LOCUS       sequence-334651-       12720 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gRNA cloning vector containing a Gal80 Co-CRISPR reporter for 
            tissue-specific CRISPR in somatic tissues..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12720)
  AUTHORS   Koreman GT, Xu Y, Hu Q, Zhang Z, Allen SE, Wolfner MF, Wang B, Han C
  TITLE     Upgraded CRISPR/Cas9 tools for tissue-specific mutagenesis in 
            Drosophila.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 Apr 6;118(14). pii: 2014255118. doi: 
            10.1073/pnas.2014255118.
  PUBMED    33782117
REFERENCE   2  (bases 1 to 12720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-04-6"; volume: "118(14). pii: 
            2014255118. doi"; pages: " 10.1073/pnas.2014255118"
COMMENT     Sequence Label: pAC-U63-QtgRNA2.1-8R
FEATURES             Location/Qualifiers
     source          1..12720
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(1..31,32..233)
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     complement(4968..4986)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(5438..5509,5911..6184,6259..6913,6975..7290,7494..7625,
                     7696..8310)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(9089..9643,9644..9674)
                     /label=P element 5' end
     rep_origin      complement(9909..10497)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9989..10008)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10668..11459,11460..11528))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11291..11310
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11529..11633)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11701..11719
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(11757..11779)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     11879..11898
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    12108..12177
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
       61 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
      121 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
      181 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttg
      241 gctgcagtcg acgctagcat atgtttgctc acctgtgatt gctcctactc aaatacaaaa
      301 acatcaaatt ttctgtcaat aaagcatatt tatttatatt tattttacag gaaagaattc
      361 cttttaaagt gtattttaac ctataatgaa aaacgattaa aaaaaataca taaaataatt
      421 cgaaaatttt tgaatagccc aggttgataa aaattcattt catacgtttt ataacttatg
      481 cccctaagta ttttttgacc atagtgtttc aattctacat taattttaca gagtagaatg
      541 aaacgccacc tactcagcca agaggcgaaa aggttagctc gccaagcaga gagggcgcca
      601 gtgctcacta ctttttataa ttctcaactt ctttttccag actcagttcg tatatataga
      661 cctattttca atttaacgtc gcaaggaaga gcgaggctct tcttgtgttt aagagctatg
      721 ctggaaacag catagcaagt ttaaataagg ctagtccgtt atcaactttg ctggaaacag
      781 caaagtggca ccgagtcggt gccagcgcgg ttccatggtg taatggttag cactcaggac
      841 tctgaatcct gcgatccgag ttcaaatctc ggtggaacct gaggttggta cggttacgag
      901 gtttaagagc tatgctggaa acagcatagc aagtttaaat aaggctagtc cgttatcaac
      961 tttgctggaa acagcaaagt ggcaccgagt cggtgctttt tttgcctacc tggagcctga
     1021 gagttgttca ataaaataaa aatgtttcgt ttttttgctt tcgccagtat ttattatttt
     1081 tcatcaatat gtattcaatt tggtatgtat ttacgcgtgg cttgctgttc ttcgcgttca
     1141 aaatctccag ctccattttg ctttcggtgc gcttgcaatc agtactgtcc aaaatcgaaa
     1201 atcgccgaac cgtagtgtga ccgtgcgggg ctctgcgaaa ataaactttt ttaggtatat
     1261 ggccacacac ggggaaagca cagtggatta tatgtattaa tatattatgc aggttttcat
     1321 tacttatcca gatgtaagcc cacttaaagc gatttaacaa ttatttgccg aaagagtata
     1381 aacaaatttc acataaaaat ggattaagaa aagcttgtgt aagattatgc gcagcgttgc
     1441 cagatagctc catttaaaac acttcaaaaa caataagttt agaaaatata tacataaata
     1501 gcagtcgttg ccgcaacgct caacacatca cacttttaaa acacccttta cctacacaga
     1561 attacttttt aaatttccag tcaagctgcg agtttcaaaa ttatagccgg tagagaagac
     1621 agtgctattt caaaagcaaa ctaacaaggg tcttaaattt caaaacacca atcctaacaa
     1681 gccttggact tttgtaagtt tagatcaaag gtggcattgc attcaatgtc atggtaagaa
     1741 gtaggtcgtc taggtagaaa tcctcattca gccggtcaag tcagtacgag aaaggtctca
     1801 atttgaaatt gtcttaaaaa tattttattg ttttgtactg tggtgagttt aaacgaaaaa
     1861 cacaaaaaaa aagtgataca cagaaatcat aaaaaatttt aatacaaggt attcgtacgt
     1921 atcaaaaaca tttcggcaca attttttttc tctgtactaa agtgttacga acactacggt
     1981 attttttagt gattttcaac ggacaccgaa ggtatataaa cagcgttcgc gaacggtcgc
     2041 cttcaaaacc aattgacatt tgcagcagca agtacaagta gaaagtaaag cgcaatcagc
     2101 gaaaaattta tacttaattg ttggtgatta aagtacaatt aaaagaacat tctcgaaagt
     2161 cacaagaaac gtaagttttt aactcgctgt taccaattag taataagagc aacaagacgt
     2221 tgagtaattt caagaaaaac tgcatttcaa ggtctttgtt cggccatttt ttttttattc
     2281 aacgctctac gtaattacaa aataagaaat tggcagccac gcatcttgtt ttcccaatga
     2341 attggcatca aaacgcaaac aaatctataa ataaaacttg cgtgttgatt ttcgccaaga
     2401 tttattggca aattgtgaaa ttcgcagtga cgcatttgaa aattcgagaa atcacgaacg
     2461 cactcgagca tttgtgtgca tgttattagt tagttgttta gttaattgaa gtattttacc
     2521 aacgaaatcc acttattttt agctgaaata gagtaggttg cttaaacaaa gccacgtctg
     2581 aaaatttctt attgcttgta gttgtgacgt caccatatac acacaaaata atgtgtatgc
     2641 atgcgtttca gctgtgtata tatacatgca cacactcgca ttatgaaaac gatgacgagc
     2701 aacggaacag gtttctcaac tacctttgtt cctgtttctt cgctttcctt tgttccaata
     2761 ttcgtagagg gttaataggg gtttctcaac aaagttggcg tcgataaata agtttcccat
     2821 ttttattccc cagccaggaa gttagtttca atagttttgt aatttcaacg aaactcattt
     2881 gatttcgtac taattttcca catctctatt ttctgcccgc agaataatcc aactgaatag
     2941 ggaattggga acttaaaaaa aaaaatcaaa atgagcggta ccgattacaa caaaaggagt
     3001 agtgtgagta cggtgccgaa tgctgctccc attcgcgtgg gcttcgtggg attgaacgcg
     3061 gctaagggtt gggccattaa aacgcattat ccagccatac tgcagctgag ctcccagttc
     3121 caaataacag cgctttattc ccccaagatc gagacgtcca ttgcgacaat tcagaggctg
     3181 aagttgtcca acgccacagc tttcccaaca ctcgagagct tcgcctcgag ctccacgatc
     3241 gatatgattg tgatcgcaat ccaggtggct tcccactacg aggtggtaat gcccctgctc
     3301 gagttttcga agaacaatcc taatctgaag tatttgtttg tggaatgggc actggcctgc
     3361 tcgctcgatc aggcggagtc gatctataag gccgctgcag agcggggcgt gcaaacaata
     3421 atcagtctgc agggacgaaa gagcccgtac attttgcggg caaaagagct tatctcccag
     3481 ggctacatcg gcgatataaa tagcatcgag attgcaggta acggaggttg gtacggttac
     3541 gagcggccgg tgaaaagccc gaaatacatt tacgagatcg gaaacggagt tgatctggtg
     3601 accaccacgt tcggtcacac gatagatata ttgcagtaca tgaccagttc gtacttcagc
     3661 cgtatcaatg ccatggtgtt taacaacatt ccagaacagg agctcattga tgagaggggc
     3721 aatcgcctgg gccagcgggt cccaaagact gtcccagatc atctcttgtt ccagggcacg
     3781 ctgctcaacg gaaatgttcc tgtgtcctgt tcctttaagg gaggcaagcc tacgaaaaag
     3841 ttcaccaaga atctggtcat agatatccat ggtacaaagg gtgatctgaa gctggagggt
     3901 gacgctggat ttgcggaaat ctcgaatctc gttctgtatt attcgggcac ccgcgccaat
     3961 gattttcccc tggccaatgg tcagcaggca cccttggacc ccggctacga cgcaggcaag
     4021 gagattatgg aggtgtatca cctccgaaac tacaacgcga tcgttggtaa tatacaccgg
     4081 ctctaccaga gtatcagcga tttccacttc aacaccaaga aaatccccga gcttccatcg
     4141 cagttcgtaa tgcaaggctt cgatttcgaa ggctttccca ccctgatgga tgccttgatc
     4201 ttgcatcgcc tgattgaaag tgtctacaag tcgaatatga tgggcagtac cctgaatgtt
     4261 tccaacatct cccactactc gctttaatct agaattaatt aactaagcca gtcggcaatc
     4321 ggacgccttc gaaacatgca acactaatgt ttaattcaga tttcagcaga gacaagctaa
     4381 aacaccgacg agttgtaatc atttctgtgc gccagcatat atttcttata tacaacgtaa
     4441 tacataatta tgtaattcta gcatctcccc aacactcaca tacaaacaaa caaaaaatac
     4501 aaacacacaa aacgtattta cccgcacgca tccttggcga ggttgagtat gaaacaaaaa
     4561 caaaacttaa tttagagcaa agtaattaca cgaataaatt taataaaaaa aaactataat
     4621 aaaaacgcca atcatgttat ttccaaaaaa aaaaaaaaaa aaactagacg agatgtttgc
     4681 gcttgtgtcg atgaatgtaa agaattgcaa ctacatagat ggtaattaat ttcagctgta
     4741 agaagtacaa gtttttcata attgtgagaa aagtgcagta gtatcgctta aagttcagtt
     4801 ttaatcaggg catttagcag ctcgaaccca atgtatttgt aagcccaata aacgtgtaaa
     4861 tgcctagagc aaaatatcac aactgtagct tttcatccga aagataataa tgaatagact
     4921 gacctgacgt ttttcaaaat gcgactagtg gcctaattct agtatgtatg taagttaata
     4981 aaacccattt ttgcggaaag tagataaaaa aaacattttt tttttttact gcactggata
     5041 tcattgaact tatctgatca gttttaaatt tacttcgatc caagggtatt tgatgtacca
     5101 ggttctttcg attacctctc actcaaaatg acattccact caaagtcagc gctgtttgcc
     5161 tccttctctg tccacagaaa tatcgccgtc tctttcgccg ctgcgtccgc tatctctttc
     5221 gccaccgttt gtagcgttac gtagcgtcaa tgtccgcctt cagttgcatt ttgtcagcgg
     5281 tttcgtgacg aagctccaag cggtttacgc catcaattaa acacaaagtg ctgtgccaaa
     5341 actcctctcg cttcttattt ttgtttgttt tttgagtgat tggggtggtg attggttttg
     5401 ggtgggtaag caggggaaag tgtgaaaaat cccggcaatg ggccaagagg atcaggagct
     5461 attaattcgc ggaggcagca aacacccatc tgccgagcat ctgaacaatg tgagtagtac
     5521 atgtgcatac atcttaagtt cacttgatct ataggaactg cgattgcaac atcaaattgt
     5581 ctgcggcgtg agaactgcga cccacaaaaa tcccaaaccg caattgcaca aacaaatagt
     5641 gacacgaaac agattattct ggtagctgtt ctcgctatat aagacaattt ttgagatcat
     5701 atcatgatca agacatctaa aggcattcat tttcgactat attctttttt acaaaaaata
     5761 taacaaccag atattttaag ctgatcctag atgcacaaaa aataaataaa agtataaacc
     5821 tacttcgtag gatacttcgg ggtacttttt gttcggggtt agatgagcat aacgcttgta
     5881 gttgatattt gagatcccct atcattgcag ggtgacagcg gagcggcttc gcagagctgc
     5941 attaaccagg gcttcgggca ggccaaaaac tacggcacgc tccggccacc cagtccgccg
     6001 gaggactccg gttcagggag cggccaacta gccgagaacc tcacctatgc ctggcacaat
     6061 atggacatct ttggggcggt caatcagccg ggctccggat ggcggcagct ggtcaaccgg
     6121 acacgcggac tattctgcaa cgagcgacac ataccggcgc ccaggaaaca tttgctcaag
     6181 aacggtgagt ttctattcgc agtcggctga tctgtgtgaa atcttaataa agggtccaat
     6241 taccaatttg aaactcagtt tgcggcgtgg cctatccggg cgaacttttg gccgtgatgg
     6301 gcagttccgg tgccggaaag acgaccctgc tgaatgccct tgcctttcga tcgccgcagg
     6361 gcatccaagt atcgccatcc gggatgcgac tgctcaatgg ccaacctgtg gacgccaagg
     6421 agatgcaggc caggtgcgcc tatgtccagc aggatgacct ctttatcggc tccctaacgg
     6481 ccagggaaca cctgattttc caagccatgg tgcggatgcc acgacatctg acctatcggc
     6541 agcgagtggc ccgcgtggat caggtgatcc aggagctttc gctcagcaaa tgtcagcaca
     6601 cgatcatcgg tgtgcccggc agggtgaaag gtctgtccgg cggagaaagg aagcgtctgg
     6661 cattcgcctc cgaggctcta accgatccgc cgcttctgat ctgcgatgag cccacctccg
     6721 gactggactc ctttaccgcc cacagcgtcg tccaggtgct gaagaagctg tcgcagaagg
     6781 gcaagaccgt catcctgacc attcatcagc cgtcttccga gctgtttgag ctctttgaca
     6841 agatccttct gatggccgag ggcagggtag ctttcttggg cactcccagc gaagccgtcg
     6901 acttcttttc ctagtgagtt cgatgtgttt attaagggta tctagtatta cataacatct
     6961 caactcctat ccagcgtggg tgcccagtgt cctaccaact acaatccggc ggacttttac
     7021 gtacaggtgt tggccgttgt gcccggacgg gagatcgagt cccgtgatcg gatcgccaag
     7081 atatgcgaca attttgccat tagcaaagta gcccgggata tggagcagtt gttggccacc
     7141 aaaaatctgg agaagccact ggagcagccg gagaatgggt acacctacaa ggccacctgg
     7201 ttcatgcagt tccgggcggt cctgtggcga tcctggctgt cggtgctcaa ggaaccactc
     7261 ctcgtaaaag tgcgacttat tcagacaacg gtgagtggtt ccagtggaaa caaatgatat
     7321 aacgcttaca attcttggaa acaaattcgc tagattttag atagaattgc ctgattccac
     7381 acccttctta gtttttttca atgagatgta tagtttatag ttttgcagaa gataaataaa
     7441 tttcatttaa ctcgcgaata ttaatgagat gcgagtaaca ttttaatttg cagatggttg
     7501 ccatcttgat tggcctcatc tttttgggcc aacaactcac gcaagtgggt gtgatgaata
     7561 tcaacggagc catcttcctc ttcctgacca acatgacctt tcaaaacgtc tttgccacga
     7621 taaatgtaag tcatgtttag aatacatttg catttcaata atttactaac tttctaatga
     7681 atcgattcga tttaggtgtt cacctcagag ctgccagttt ttatgaggga ggcccgaagt
     7741 cgactttatc gctgtgacac atactttctg ggcaaaacga ttgccgaatt gccgcttttt
     7801 ctcacagtgc cactggtctt cacggcgatt gcctatccga tgatcggact gcgggccgga
     7861 gtgctgcact tcttcaactg cctggcgctg gtcactctgg tggccaatgt gtcaacgtcc
     7921 ttcggatatc taatatcctg cgccagctcc tcgacctcga tggcgctgtc tgtgggtccg
     7981 ccggttatca taccattcct gctctttggc ggcttcttct tgaactcggg ctcggtgcca
     8041 gtatacctca aatggttgtc gtacctctca tggttccgtt acgccaacga gggtctgctg
     8101 attaaccaat gggcggacgt ggagccgggc gaaattagct gcacatcgtc gaacaccacg
     8161 tgccccagtt cgggcaaggt catcctggag acgcttaact tctccgccgc cgatctgccg
     8221 ctggactacg tgggtctggc cattctcatc gtgagcttcc gggtgctcgc atatctggct
     8281 ctaagacttc gggcccgacg caaggagtag ccgacatata tccgaaataa ctgcttgttt
     8341 ttttttttta ccattattac catcgtgttt actgtttatt gccccctcaa aaagctaatg
     8401 taattatatt tgtgccaata aaaacaagat atgacctata gaatacaagt atttcccctt
     8461 cgaacatccc cacaagtaga ctttggattt gtcttctaac caaaagactt acacacctgc
     8521 ataccttaca tcaaaaactc gtttatcgct acataaaaca ccgggatata ttttttatat
     8581 acatactttt caaatcgcgc gccctcttca taattcacct ccaccacacc acgtttcgta
     8641 gttgctcttt cgctgtctcc cacccgctct ccgcaacaca ttcacctttt gttcgacgac
     8701 cttggagcga ctgtcgttag ttccgcgcga ttcggttcgc tcaaatggtt ccgagtggtt
     8761 catttcgtct caatagaaat tagtaataaa tatttgtatg tacaatttat ttgctccaat
     8821 atatttgtat atatttccct cacagctata tttattctaa tttaatatta tgacttttta
     8881 aggtaatttt ttgtgacctg ttcggagtga ttagcgttac aatttgaact gaaagtgaca
     8941 tccagtgttt gttccttgtg tagatgcatc tcaaaaaaat ggtgggcata atagtgttgt
     9001 ttatatatat caaaaataac aactataata ataagaatac atttaattta gaaaatgctt
     9061 ggatttcact ggaactagaa ttaattcggc tgctgctcta aacgacgcat ttcgtactcc
     9121 aaagtacgaa ttttttccct caagctctta ttttcattaa acaatgaaca ggacctaacg
     9181 cacagtcacg ttattgttta cataaatgat tttttttact attcaaactt actctgtttg
     9241 tgtactccca ctggtatagc cttcttttat cttttctggt tcaggctcta tcactttact
     9301 aggtacggca tctgcgttga gtcgcctcct tttaaatgtc tgaccttttg caggtgcagc
     9361 cttccactgc gaatcattaa agtgggtatc acaaatttgg gagttttcac caaggctgca
     9421 cccaaggctc tgctcccaca attttctctt aatagcacac ttcggcacgt gaattaattt
     9481 tactccagtc acagctttgc agcaaaattt gcaatatttc attttttttt attccacgta
     9541 agggttaatg ttttcaaaaa aaaattcgtc cgcacacaac ctttcctctc aacaagcaaa
     9601 cgtgcactga atttaagtgt atacttcggt aagcttcggc tatcgacggg accaccttat
     9661 gttatttcat catgggccag acccacgtag tccagcggca gatcggcggc ggagaagtta
     9721 agcgtctcca ggatgacctt gcccgaactg gggcacgtgg tgttcgacga tgtgcagcta
     9781 atttcgcccg gctccacgtc cgcccattgg ttaatcagca gaccctcgtt ggcgtaacgg
     9841 aaccatgaga ggtacgacaa ccatttgagg tatactggca ccgagcccga gttcaagaag
     9901 aaggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     9961 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
    10021 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
    10081 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
    10141 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
    10201 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
    10261 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
    10321 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
    10381 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
    10441 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
    10501 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
    10561 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
    10621 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
    10681 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
    10741 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
    10801 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
    10861 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
    10921 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
    10981 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
    11041 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
    11101 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
    11161 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
    11221 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
    11281 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
    11341 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
    11401 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
    11461 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
    11521 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
    11581 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
    11641 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
    11701 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
    11761 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
    11821 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
    11881 gcatcagagc agattgtact gagagtgcac catatcgaca tgcccgccgt gaccgtcgag
    11941 aacccgctga cgctgccccg cgtatccgca cccgccgacg ccgtcgcacg tcccgtgctc
    12001 accgtgacca ccgcgcccag cggtttcgag ggcgagggct tcccggtgcg ccgcgcgttc
    12061 gccgggatca actaccgcca cctcgacccg ttcatcatga tggaccagat gggtgaggtg
    12121 gagtacgcgc ccggggagcc caagggcacg ccctggcacc cgcaccgcgg cttcgagacc
    12181 gtgacctaca tcgtcgatat gcggtgtgaa ataccgcacc gaatcgcgcg gaactaacga
    12241 cagtcgctcc aaggtcgtcg aacaaaaggt gaatgtgttg cggagagcgg gtgggagaca
    12301 gcgaaagagc aactacgaaa cgtggtgtgg tggaggtgaa ttatgaagag ggcgcgcgat
    12361 ttgaaaagta tgtatataaa aaatatatcc cggtgtttta tgtagcgata aacgagtttt
    12421 tgatgtaagg tatgcaggtg tgtaagtctt ttggttagaa gacaaatcca aagtctactt
    12481 gtggggatgt tcgaagggga aatacttgta ttctataggt catatcttgt ttttattggc
    12541 acaaatataa ttacattagc tttttgaggg ggcaataaac agtaaacacg atggtaataa
    12601 tggtaaaaaa aaaaaacaag cagttatttc ggatatatgt cggctactcc ttgcgtcggg
    12661 cccgaagtct tagagccaga tatgcgagca cccggaagct cacgatgaga atggccagac
//
