LOCUS       sequence-334653-        3870 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC17A2_STOP
FEATURES             Location/Qualifiers
     source          1..3870
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1427..1526)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1543..1562)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1544..1562)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1567..1583)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1567..1583)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1685..1704)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1696..2505
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1763..1782)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2652..3240
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3141..3160
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3394..3411
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3570..3597)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3689..3775)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3824..3846
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3838..3855
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3839..3855
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgga cggcaagcca
      121 gcaaccagga agggacctga tttttgctcc ctgcgctacg gcctggccct gatcatgcac
      181 ttctctaact ttaccatgat cacacagcgg gtgagcctgt ccatcgccat catcgccatg
      241 gtgaacacca cacagcagca gggcctgtcc aatgcatcta ccgagggacc agtggcagac
      301 gccttcaaca atagctccat cagcatcaag gagtttgata ccaaggccag cgtgtaccag
      361 tggagcccag agacacaggg catcatcttt tctagcatca attacggcat catcctgaca
      421 ctgatcccct ccggctatct ggccggcatc ttcggagcca agaagatgct gggagcagga
      481 ctgctgatct cctctctgct gaccctgttc acacccctgg ccgccgactt tggcgtgatc
      541 ctggtcatca tggtgcggac cgtgcaggga atggcacagg gaatggcatg gaccggccag
      601 ttcacaatct gggcaaagtg ggcaccacct ctggagagaa gcaagctgac cacaatcgca
      661 ggatctggaa gcgccttcgg ctcctttatc atcctgtgcg tgggaggact gatcagccag
      721 gccctgtcct ggcctttcat cttttacatc ttcggctcca ccggctgcgt gtgctgtctg
      781 ctgtggttta cagtgatcta tgacgatcct atgcaccacc catgtatctc tgtgagggag
      841 aaggagcaca tcctgagctc cctggcacag cagccatcta gcccaggaag ggcagtgcct
      901 atcaaggcaa tggtgacatg cctgccactg tgggccatct tcctgggctt ctttagccac
      961 ttttggctgt gcaccatcat cctgacatac ctgcctacct atatctctac actgctgcac
     1021 gtgaacatca gggacagcgg cgtgctgtcc tctctgccat tcatcgcagc agcatcttgt
     1081 accatcctgg gaggccagct ggccgatttt ctgctgagcc ggaatctgct gagactgatc
     1141 acagtgcgca agctgttcag ctccctgggc ctgctgctgc caagcatctg cgcagtggcc
     1201 ctgccctttg tggcatctag ctacgtgatc accatcatcc tgctgatcct gatccccggc
     1261 acatccaacc tgtgcgactc tggcttcatc atcaataccc tggatatcgc ccctcggtat
     1321 gccagcttcc tgatgggcat ctccagaggc tttggcctga tcgccggcat catctcctct
     1381 accgccacag gcttcctgat ctcccaagtg ggccccgtgt actaagaccc agctttcttg
     1441 tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga acaggtcact
     1501 atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag tgagtcgtat
     1561 tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac
     1621 attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt
     1681 aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg attaaattcc
     1741 aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt
     1801 gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct gaaacatggc
     1861 aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa
     1921 tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc
     1981 accactgcga tccccggaaa aacagcattc caggtattag aagaatatcc tgattcaggt
     2041 gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt
     2101 aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat
     2161 aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa
     2221 gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt cactcatggt
     2281 gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt
     2341 ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt
     2401 gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat
     2461 atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat
     2521 tggttgtaac actggcagag cattacgctg acttgacggg acggcgcaag ctcatgacca
     2581 aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc
     2641 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     2701 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     2761 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     2821 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     2881 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     2941 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     3001 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     3061 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     3121 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     3181 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     3241 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     3301 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     3361 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     3421 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     3481 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc
     3541 gctagccagg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct
     3601 tagtttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct
     3661 tcacaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca
     3721 aacaacagat aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc
     3781 tggcagttcc ctactctcgc gttaacgcta gcatggatgt tttcccagtc acgacgttgt
     3841 aaaacgacgg ccagtcttaa gctcgggccc
//
