LOCUS       sequence-334655-        3789 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-MFSD3_STOP
FEATURES             Location/Qualifiers
     source          1..3789
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1346..1445)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1462..1481)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1463..1481)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1486..1502)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1486..1502)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1604..1623)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1615..2424
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1682..1701)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2571..3159
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3060..3079
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3313..3330
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3489..3516)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3608..3694)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3743..3765
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3757..3774
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3758..3774
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgag gggcaagctg
      121 ctgcctctgg caggactgta cctggtgcag ggactgccat atggactgca gtccggactg
      181 ctgcctgtgc tgctgagggc aggcggcctg tctctgacca gagtgggcct ggccaaggtg
      241 ctgtacgcac catggctgct gaagctggca tgggcaccac tggtggacgc acagggcagc
      301 gccagggcat gggtgaccag atccacagca ggactgggac tggtgtgcgg cctgctggcc
      361 ggcctgcccc ctccaggagc aggacaggca ggactgccag cagcagtggc aggactgctg
      421 ctgctgctga acctgggagc agcaatgcag gacgtggccc tggatgcact ggcagtgcag
      481 ctgctggagc ctgcagagct gggcccaggc aataccgtgc aggtggtggc ctacaagctg
      541 ggagccgccc tggcaggagg cgccctgctg gccctgctgc ccaccttcag ctggcctcag
      601 ctgtttctgc tgctggcagc aacatattgg ctggcagccg ccctggcatg ggcagcacca
      661 gccctgcgga gactgccaca gcagccacct tccgagcaga ggccacacac cgcacacctg
      721 ctgagggacg tgctggcagt gcctggaacc gtgtggacag caggcttcgt gctgacatat
      781 aagctgggag agcagggagc aagctccctg tttcctctgc tgctgctgga tcacggcgtg
      841 tctgccccag agctgggact gtggaacgga gtgggagcag tggtgtgcag catcgcagga
      901 tctagcctgg gaggcaccct gctggcaaag cactggaagc tgctgccact gctgcggtcc
      961 gtgctgaggt tccgcctggg aggactggca tgtcagaccg ccctggtgtt tcacctggac
     1021 acactgggcg cctctatgga tgccggcaca atcctgagag gctccgccct gctgtctctg
     1081 tgcctgcagc acttcctggg cggcctggtg accacagtga cctttacagg catgatgagg
     1141 tgtagccagc tggcaccacg cgccctgcag gcaacccact actccctgct ggccacactg
     1201 gagctgctgg gcaagctgct gctgggcacc ctggcaggag gactggcaga cggactggga
     1261 ccacacccct gtttcctgct gctgctgatc ctgtctgcct ttcctgtgct gtatctggat
     1321 ctggccccaa gcacattcct gtaagaccca gctttcttgt acaaagttgg cattataaga
     1381 aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt
     1441 atttgccatc cagctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc
     1501 tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata
     1561 tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc
     1621 catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc tgatttatat
     1681 gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgcttgtat
     1741 gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat
     1801 gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc
     1861 aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat ccccggaaaa
     1921 acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg
     1981 gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat
     2041 cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt
     2101 gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa
     2161 cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt
     2221 atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac
     2281 cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag
     2341 aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat
     2401 ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc
     2461 attacgctga cttgacggga cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac
     2521 gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     2581 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     2641 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     2701 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     2761 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     2821 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     2881 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     2941 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     3001 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     3061 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     3121 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     3181 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     3241 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     3301 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     3361 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     3421 aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga agagtttgta
     3481 gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt
     3541 atggcgggcg tcctgcccgc caccctccgg gccgttgctt cacaacgttc aaatccgctc
     3601 ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg
     3661 cccagtcttc cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgcg
     3721 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaag
     3781 ctcgggccc
//
