LOCUS       sequence-334663-       12770 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gRNA cloning vector containing a CR7T promoter, gRNA(2.1) scaffold, 
            and a Ubi-mTagBFP-NLS marker; optimized for germline CRISPR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12770)
  AUTHORS   Koreman GT, Xu Y, Hu Q, Zhang Z, Allen SE, Wolfner MF, Wang B, Han C
  TITLE     Upgraded CRISPR/Cas9 tools for tissue-specific mutagenesis in 
            Drosophila.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 Apr 6;118(14). pii: 2014255118. doi: 
            10.1073/pnas.2014255118.
  PUBMED    33782117
REFERENCE   2  (bases 1 to 12770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-04-6"; volume: "118(14). pii: 
            2014255118. doi"; pages: " 10.1073/pnas.2014255118"
COMMENT     Sequence Label: pAC-CR7T-gRNA2.1-nlsBFP
FEATURES             Location/Qualifiers
     source          1..12770
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(1..31,32..233)
                     /label=P element 3' end
                     /note="P element 3' end"
     CDS             3561..3581
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5018..5036)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(5488..5559,5961..6234,6309..6963,7025..7340,7544..7675,
                     7746..8360)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(9139..9693,9694..9724)
                     /label=P element 5' end
     rep_origin      complement(9959..10547)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10039..10058)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10718..11509,11510..11578))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11341..11360
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11579..11683)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11751..11769
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(11807..11829)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     11929..11948
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    12158..12227
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
       61 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
      121 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
      181 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttg
      241 gctgcagact agtggatcct taattaaggt accagatctg ctagcaaagt gaacacgtcg
      301 ctaagcgaaa gcccgttttg tcatcgcttt ttgtcgggtc tcagttgtgg atcgaaaacc
      361 cggagagtaa ccccttgacc ccttgtcagt ttccgcatgc tccagccctt gtcctcatat
      421 tttccaatcg tttttggcgt ttttagctgc ggccattgaa tgttcaaaaa ttattgtaaa
      481 cttttcagac cccctccgtg tgtgtgtgtg tgtgtgtgct tctgtgtatg tggaaaagtt
      541 cgaattctag ttgggaaatc aattaaaagc gcttgttgga ctcgcaccac aactttccct
      601 ttcgatcgcc cctgcggctt ggttcaactg gtcaaggcct ctagctcccg aaaaccgtcg
      661 aaaattggtt tggcactttt tatagccacc caatataata ccaagttcag acagaaattg
      721 atatgggtat tggactgatc aataacttaa gttccctcac tgaattatcc ttaactcttg
      781 tgcatgccta tttatgtgaa aacaatcaaa attactcaat tatgtatgca caggtattga
      841 aagctcccac tatttagttt ttgttttaat tatctgtctt tagaacttta aatggaaaaa
      901 catgacttat atgtatgaac aaaaattcca atcgatcact ttaggcggcc gccaatgcca
      961 acagaagcat tatttttttc ctttcccgat ctctcaagca cactcagttg atctcagtcg
     1021 caaaactcgt tactcactct taatatttcc tcttctttgc actttcctgg tggaatgcat
     1081 tctctatttt cctctcccta ctgcacatac tctttgagct ctctagcaga taccctaaca
     1141 atttctaatc atccacgaat aataaacaaa gatttctaat atcttaaacc tgttttatat
     1201 acttattatt caaaaaaaaa agtgtggcaa aaaaaaagag cacttcaacg tttgttgaac
     1261 aaaggatata attttgtaag ggtatctaga agttttcatg cctctcttca tccactcttc
     1321 tcaacctcat gcgatagctg ctgcgctctc tcgtttatcg ttcggttgag gttttataat
     1381 tctcaaatac tttttcccga agtggtgcct ttaaatagcg tatatgagtg gaagactttc
     1441 gcaaggaaga gcgaggctct tcttgtgttt aagagctatg ctggaaacag catagcaagt
     1501 ttaaataagg ctagtccgtt atcaactttg ctggaaacag caaagtggca ccgagtcggt
     1561 gctttttttg cctacctgga gcctgagagt tgttcaataa aataaaaatg tttcgttttt
     1621 ttgctttcgc cagtatttat tatttttcat caatatgtat tcaatttggt atgtatttac
     1681 gcgtggcttg ctgttcttcg cgttcaaaat ctccagctcc attttgcttt cggtgcgctt
     1741 gcaatcagta ctgtccaaaa tcgaaaatcg ccgaaccgta gtgtgaccgt gcggggctct
     1801 gcgaaaataa acttttttag gtatatggcc acacacgggg aaagcacagt ggattatatg
     1861 tattaatata ttatgcaggt tttcattact tatccagatg taagcccact taaagcgatt
     1921 taacaattat ttgccgaaag agtataaaca aatttcacat aaaaatggat taagaaaagc
     1981 ttgtgtaaga ttatgcgcag cgttgccaga tagctccatt taaaacactt caaaaacaat
     2041 aagtttagaa aatatataca taaatagcag tcgttgccgc aacgctcaac acatcacact
     2101 tttaaaacac cctttaccta cacagaatta ctttttaaat ttccagtcaa gctgcgagtt
     2161 tcaaaattat agccggtaga gaagacagtg ctatttcaaa agcaaactaa caagggtctt
     2221 aaatttcaaa acaccaatcc taacaagcct tggacttttg taagtttaga tcaaaggtgg
     2281 cattgcattc aatgtcatgg taagaagtag gtcgtctagg tagaaatcct cattcagccg
     2341 gtcaagtcag tacgagaaag gtctcaattt gaaattgtct taaaaatatt ttattgtttt
     2401 gtactgtggt gagtttaaac gaaaaacaca aaaaaaaagt gatacacaga aatcataaaa
     2461 aattttaata caaggtattc gtacgtatca aaaacatttc ggcacaattt tttttctctg
     2521 tactaaagtg ttacgaacac tacggtattt tttagtgatt ttcaacggac accgaaggta
     2581 tataaacagc gttcgcgaac ggtcgccttc aaaaccaatt gacatttgca gcagcaagta
     2641 caagtagaaa gtaaagcgca atcagcgaaa aatttatact taattgttgg tgattaaagt
     2701 acaattaaaa gaacattctc gaaagtcaca agaaacgtaa gtttttaact cgctgttacc
     2761 aattagtaat aagagcaaca agacgttgag taatttcaag aaaaactgca tttcaaggtc
     2821 tttgttcggc catttttttt ttattcaacg ctctacgtaa ttacaaaata agaaattggc
     2881 agccacgcat cttgttttcc caatgaattg gcatcaaaac gcaaacaaat ctataaataa
     2941 aacttgcgtg ttgattttcg ccaagattta ttggcaaatt gtgaaattcg cagtgacgca
     3001 tttgaaaatt cgagaaatca cgaacgcact cgagcatttg tgtgcatgtt attagttagt
     3061 tgtttagtta attgaagtat tttaccaacg aaatccactt atttttagct gaaatagagt
     3121 aggttgctta aacaaagcca cgtctgaaaa tttcttattg cttgtagttg tgacgtcacc
     3181 atatacacac aaaataatgt gtatgcatgc gtttcagctg tgtatatata catgcacaca
     3241 ctcgcattat gaaaacgatg acgagcaacg gaacaggttt ctcaactacc tttgttcctg
     3301 tttcttcgct ttcctttgtt ccaatattcg tagagggtta ataggggttt ctcaacaaag
     3361 ttggcgtcga taaataagtt tcccattttt attccccagc caggaagtta gtttcaatag
     3421 ttttgtaatt tcaacgaaac tcatttgatt tcgtactaat tttccacatc tctattttct
     3481 gcccgcagaa taatccaact gaatagggaa ttgggaactt aaaaaaaaaa atcaaaatga
     3541 gcggtaccga tcgcagcccc cccaagaaga agcgcaaggt ggagttcgga tccggtggcg
     3601 gcggaagtgg aggtggaggc tcgatggtgt ccaagggcga ggagctgatc aaggagaaca
     3661 tgcacatgaa gctgtacatg gagggcaccg tggacaacca ccacttcaag tgcacctccg
     3721 agggcgaggg caagccctac gagggcaccc agaccatgcg catcaaggtg gtggagggcg
     3781 gccccctgcc cttcgccttc gacatcctgg ccacctcctt cctgtacggc tccaagacct
     3841 tcatcaacca cacccagggc atccccgact tcttcaagca gtccttcccc gagggcttca
     3901 cctgggagcg cgtgaccacc tacgaggacg gcggcgtgct gaccgccacc caggacacct
     3961 ccctgcaaga cggctgcctg atctacaacg tgaagatccg cggcgtgaac ttcacctcca
     4021 acggccccgt gatgcagaag aagaccctgg gctgggaggc cttcaccgag accctgtacc
     4081 ccgccgacgg cggcctggag ggccgcaacg acatggccct gaagctggtg ggcggctccc
     4141 acctgatcgc caacgccaag accacctacc gctccaagaa gcccgccaag aacctgaaga
     4201 tgcccggcgt gtactacgtg gactaccgcc tggagcgcat caaggaggcc aacaacgaga
     4261 cctacgtgga gcagcacgag gtggccgtgg cccgctactg cgacctgccc tccaagctgg
     4321 gccacaagct gaactaatct agaattaatt aactaagcca gtcggcaatc ggacgccttc
     4381 gaaacatgca acactaatgt ttaattcaga tttcagcaga gacaagctaa aacaccgacg
     4441 agttgtaatc atttctgtgc gccagcatat atttcttata tacaacgtaa tacataatta
     4501 tgtaattcta gcatctcccc aacactcaca tacaaacaaa caaaaaatac aaacacacaa
     4561 aacgtattta cccgcacgca tccttggcga ggttgagtat gaaacaaaaa caaaacttaa
     4621 tttagagcaa agtaattaca cgaataaatt taataaaaaa aaactataat aaaaacgcca
     4681 atcatgttat ttccaaaaaa aaaaaaaaaa aaactagacg agatgtttgc gcttgtgtcg
     4741 atgaatgtaa agaattgcaa ctacatagat ggtaattaat ttcagctgta agaagtacaa
     4801 gtttttcata attgtgagaa aagtgcagta gtatcgctta aagttcagtt ttaatcaggg
     4861 catttagcag ctcgaaccca atgtatttgt aagcccaata aacgtgtaaa tgcctagagc
     4921 aaaatatcac aactgtagct tttcatccga aagataataa tgaatagact gacctgacgt
     4981 ttttcaaaat gcgactagtg gcctaattct agtatgtatg taagttaata aaacccattt
     5041 ttgcggaaag tagataaaaa aaacattttt tttttttact gcactggata tcattgaact
     5101 tatctgatca gttttaaatt tacttcgatc caagggtatt tgatgtacca ggttctttcg
     5161 attacctctc actcaaaatg acattccact caaagtcagc gctgtttgcc tccttctctg
     5221 tccacagaaa tatcgccgtc tctttcgccg ctgcgtccgc tatctctttc gccaccgttt
     5281 gtagcgttac gtagcgtcaa tgtccgcctt cagttgcatt ttgtcagcgg tttcgtgacg
     5341 aagctccaag cggtttacgc catcaattaa acacaaagtg ctgtgccaaa actcctctcg
     5401 cttcttattt ttgtttgttt tttgagtgat tggggtggtg attggttttg ggtgggtaag
     5461 caggggaaag tgtgaaaaat cccggcaatg ggccaagagg atcaggagct attaattcgc
     5521 ggaggcagca aacacccatc tgccgagcat ctgaacaatg tgagtagtac atgtgcatac
     5581 atcttaagtt cacttgatct ataggaactg cgattgcaac atcaaattgt ctgcggcgtg
     5641 agaactgcga cccacaaaaa tcccaaaccg caattgcaca aacaaatagt gacacgaaac
     5701 agattattct ggtagctgtt ctcgctatat aagacaattt ttgagatcat atcatgatca
     5761 agacatctaa aggcattcat tttcgactat attctttttt acaaaaaata taacaaccag
     5821 atattttaag ctgatcctag atgcacaaaa aataaataaa agtataaacc tacttcgtag
     5881 gatacttcgg ggtacttttt gttcggggtt agatgagcat aacgcttgta gttgatattt
     5941 gagatcccct atcattgcag ggtgacagcg gagcggcttc gcagagctgc attaaccagg
     6001 gcttcgggca ggccaaaaac tacggcacgc tccggccacc cagtccgccg gaggactccg
     6061 gttcagggag cggccaacta gccgagaacc tcacctatgc ctggcacaat atggacatct
     6121 ttggggcggt caatcagccg ggctccggat ggcggcagct ggtcaaccgg acacgcggac
     6181 tattctgcaa cgagcgacac ataccggcgc ccaggaaaca tttgctcaag aacggtgagt
     6241 ttctattcgc agtcggctga tctgtgtgaa atcttaataa agggtccaat taccaatttg
     6301 aaactcagtt tgcggcgtgg cctatccggg cgaacttttg gccgtgatgg gcagttccgg
     6361 tgccggaaag acgaccctgc tgaatgccct tgcctttcga tcgccgcagg gcatccaagt
     6421 atcgccatcc gggatgcgac tgctcaatgg ccaacctgtg gacgccaagg agatgcaggc
     6481 caggtgcgcc tatgtccagc aggatgacct ctttatcggc tccctaacgg ccagggaaca
     6541 cctgattttc caagccatgg tgcggatgcc acgacatctg acctatcggc agcgagtggc
     6601 ccgcgtggat caggtgatcc aggagctttc gctcagcaaa tgtcagcaca cgatcatcgg
     6661 tgtgcccggc agggtgaaag gtctgtccgg cggagaaagg aagcgtctgg cattcgcctc
     6721 cgaggctcta accgatccgc cgcttctgat ctgcgatgag cccacctccg gactggactc
     6781 ctttaccgcc cacagcgtcg tccaggtgct gaagaagctg tcgcagaagg gcaagaccgt
     6841 catcctgacc attcatcagc cgtcttccga gctgtttgag ctctttgaca agatccttct
     6901 gatggccgag ggcagggtag ctttcttggg cactcccagc gaagccgtcg acttcttttc
     6961 ctagtgagtt cgatgtgttt attaagggta tctagtatta cataacatct caactcctat
     7021 ccagcgtggg tgcccagtgt cctaccaact acaatccggc ggacttttac gtacaggtgt
     7081 tggccgttgt gcccggacgg gagatcgagt cccgtgatcg gatcgccaag atatgcgaca
     7141 attttgccat tagcaaagta gcccgggata tggagcagtt gttggccacc aaaaatctgg
     7201 agaagccact ggagcagccg gagaatgggt acacctacaa ggccacctgg ttcatgcagt
     7261 tccgggcggt cctgtggcga tcctggctgt cggtgctcaa ggaaccactc ctcgtaaaag
     7321 tgcgacttat tcagacaacg gtgagtggtt ccagtggaaa caaatgatat aacgcttaca
     7381 attcttggaa acaaattcgc tagattttag atagaattgc ctgattccac acccttctta
     7441 gtttttttca atgagatgta tagtttatag ttttgcagaa gataaataaa tttcatttaa
     7501 ctcgcgaata ttaatgagat gcgagtaaca ttttaatttg cagatggttg ccatcttgat
     7561 tggcctcatc tttttgggcc aacaactcac gcaagtgggt gtgatgaata tcaacggagc
     7621 catcttcctc ttcctgacca acatgacctt tcaaaacgtc tttgccacga taaatgtaag
     7681 tcatgtttag aatacatttg catttcaata atttactaac tttctaatga atcgattcga
     7741 tttaggtgtt cacctcagag ctgccagttt ttatgaggga ggcccgaagt cgactttatc
     7801 gctgtgacac atactttctg ggcaaaacga ttgccgaatt gccgcttttt ctcacagtgc
     7861 cactggtctt cacggcgatt gcctatccga tgatcggact gcgggccgga gtgctgcact
     7921 tcttcaactg cctggcgctg gtcactctgg tggccaatgt gtcaacgtcc ttcggatatc
     7981 taatatcctg cgccagctcc tcgacctcga tggcgctgtc tgtgggtccg ccggttatca
     8041 taccattcct gctctttggc ggcttcttct tgaactcggg ctcggtgcca gtatacctca
     8101 aatggttgtc gtacctctca tggttccgtt acgccaacga gggtctgctg attaaccaat
     8161 gggcggacgt ggagccgggc gaaattagct gcacatcgtc gaacaccacg tgccccagtt
     8221 cgggcaaggt catcctggag acgcttaact tctccgccgc cgatctgccg ctggactacg
     8281 tgggtctggc cattctcatc gtgagcttcc gggtgctcgc atatctggct ctaagacttc
     8341 gggcccgacg caaggagtag ccgacatata tccgaaataa ctgcttgttt ttttttttta
     8401 ccattattac catcgtgttt actgtttatt gccccctcaa aaagctaatg taattatatt
     8461 tgtgccaata aaaacaagat atgacctata gaatacaagt atttcccctt cgaacatccc
     8521 cacaagtaga ctttggattt gtcttctaac caaaagactt acacacctgc ataccttaca
     8581 tcaaaaactc gtttatcgct acataaaaca ccgggatata ttttttatat acatactttt
     8641 caaatcgcgc gccctcttca taattcacct ccaccacacc acgtttcgta gttgctcttt
     8701 cgctgtctcc cacccgctct ccgcaacaca ttcacctttt gttcgacgac cttggagcga
     8761 ctgtcgttag ttccgcgcga ttcggttcgc tcaaatggtt ccgagtggtt catttcgtct
     8821 caatagaaat tagtaataaa tatttgtatg tacaatttat ttgctccaat atatttgtat
     8881 atatttccct cacagctata tttattctaa tttaatatta tgacttttta aggtaatttt
     8941 ttgtgacctg ttcggagtga ttagcgttac aatttgaact gaaagtgaca tccagtgttt
     9001 gttccttgtg tagatgcatc tcaaaaaaat ggtgggcata atagtgttgt ttatatatat
     9061 caaaaataac aactataata ataagaatac atttaattta gaaaatgctt ggatttcact
     9121 ggaactagaa ttaattcggc tgctgctcta aacgacgcat ttcgtactcc aaagtacgaa
     9181 ttttttccct caagctctta ttttcattaa acaatgaaca ggacctaacg cacagtcacg
     9241 ttattgttta cataaatgat tttttttact attcaaactt actctgtttg tgtactccca
     9301 ctggtatagc cttcttttat cttttctggt tcaggctcta tcactttact aggtacggca
     9361 tctgcgttga gtcgcctcct tttaaatgtc tgaccttttg caggtgcagc cttccactgc
     9421 gaatcattaa agtgggtatc acaaatttgg gagttttcac caaggctgca cccaaggctc
     9481 tgctcccaca attttctctt aatagcacac ttcggcacgt gaattaattt tactccagtc
     9541 acagctttgc agcaaaattt gcaatatttc attttttttt attccacgta agggttaatg
     9601 ttttcaaaaa aaaattcgtc cgcacacaac ctttcctctc aacaagcaaa cgtgcactga
     9661 atttaagtgt atacttcggt aagcttcggc tatcgacggg accaccttat gttatttcat
     9721 catgggccag acccacgtag tccagcggca gatcggcggc ggagaagtta agcgtctcca
     9781 ggatgacctt gcccgaactg gggcacgtgg tgttcgacga tgtgcagcta atttcgcccg
     9841 gctccacgtc cgcccattgg ttaatcagca gaccctcgtt ggcgtaacgg aaccatgaga
     9901 ggtacgacaa ccatttgagg tatactggca ccgagcccga gttcaagaag aaggcgtttt
     9961 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
    10021 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
    10081 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
    10141 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
    10201 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
    10261 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
    10321 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
    10381 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
    10441 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
    10501 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
    10561 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
    10621 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
    10681 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
    10741 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
    10801 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
    10861 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
    10921 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
    10981 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
    11041 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
    11101 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
    11161 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
    11221 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
    11281 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
    11341 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
    11401 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
    11461 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
    11521 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    11581 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    11641 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    11701 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
    11761 tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
    11821 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
    11881 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc
    11941 agattgtact gagagtgcac catatcgaca tgcccgccgt gaccgtcgag aacccgctga
    12001 cgctgccccg cgtatccgca cccgccgacg ccgtcgcacg tcccgtgctc accgtgacca
    12061 ccgcgcccag cggtttcgag ggcgagggct tcccggtgcg ccgcgcgttc gccgggatca
    12121 actaccgcca cctcgacccg ttcatcatga tggaccagat gggtgaggtg gagtacgcgc
    12181 ccggggagcc caagggcacg ccctggcacc cgcaccgcgg cttcgagacc gtgacctaca
    12241 tcgtcgatat gcggtgtgaa ataccgcacc gaatcgcgcg gaactaacga cagtcgctcc
    12301 aaggtcgtcg aacaaaaggt gaatgtgttg cggagagcgg gtgggagaca gcgaaagagc
    12361 aactacgaaa cgtggtgtgg tggaggtgaa ttatgaagag ggcgcgcgat ttgaaaagta
    12421 tgtatataaa aaatatatcc cggtgtttta tgtagcgata aacgagtttt tgatgtaagg
    12481 tatgcaggtg tgtaagtctt ttggttagaa gacaaatcca aagtctactt gtggggatgt
    12541 tcgaagggga aatacttgta ttctataggt catatcttgt ttttattggc acaaatataa
    12601 ttacattagc tttttgaggg ggcaataaac agtaaacacg atggtaataa tggtaaaaaa
    12661 aaaaaacaag cagttatttc ggatatatgt cggctactcc ttgcgtcggg cccgaagtct
    12721 tagagccaga tatgcgagca cccggaagct cacgatgaga atggccagac
//
