LOCUS       sequence-334666-       12204 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gRNA cloning vector containing a BFP Co-CRISPR reporter for 
            tissue-specific CRISPR in somatic tissues..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12204)
  AUTHORS   Koreman GT, Xu Y, Hu Q, Zhang Z, Allen SE, Wolfner MF, Wang B, Han C
  TITLE     Upgraded CRISPR/Cas9 tools for tissue-specific mutagenesis in 
            Drosophila.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 Apr 6;118(14). pii: 2014255118. doi: 
            10.1073/pnas.2014255118.
  PUBMED    33782117
REFERENCE   2  (bases 1 to 12204)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-04-6"; volume: "118(14). pii: 
            2014255118. doi"; pages: " 10.1073/pnas.2014255118"
COMMENT     Sequence Label: pAC-U63-QtgRNA2.1-BR
FEATURES             Location/Qualifiers
     source          1..12204
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(1..31,32..233)
                     /label=P element 3' end
                     /note="P element 3' end"
     CDS             2995..3015
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(4452..4470)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(4922..4993,5395..5668,5743..6397,6459..6774,6978..7109,
                     7180..7794)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(8573..9127,9128..9158)
                     /label=P element 5' end
     rep_origin      complement(9393..9981)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9473..9492)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10152..10943,10944..11012))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10775..10794
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11013..11117)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11185..11203
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(11241..11263)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     11363..11382
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    11592..11661
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
       61 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
      121 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
      181 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttg
      241 gctgcagtcg acgctagcat atgtttgctc acctgtgatt gctcctactc aaatacaaaa
      301 acatcaaatt ttctgtcaat aaagcatatt tatttatatt tattttacag gaaagaattc
      361 cttttaaagt gtattttaac ctataatgaa aaacgattaa aaaaaataca taaaataatt
      421 cgaaaatttt tgaatagccc aggttgataa aaattcattt catacgtttt ataacttatg
      481 cccctaagta ttttttgacc atagtgtttc aattctacat taattttaca gagtagaatg
      541 aaacgccacc tactcagcca agaggcgaaa aggttagctc gccaagcaga gagggcgcca
      601 gtgctcacta ctttttataa ttctcaactt ctttttccag actcagttcg tatatataga
      661 cctattttca atttaacgtc gcaaggaaga gcgaggctct tcttgtgttt aagagctatg
      721 ctggaaacag catagcaagt ttaaataagg ctagtccgtt atcaactttg ctggaaacag
      781 caaagtggca ccgagtcggt gccagcgcgg ttccatggtg taatggttag cactcaggac
      841 tctgaatcct gcgatccgag ttcaaatctc ggtggaacct gtgaccacct acgaggacgg
      901 gtttaagagc tatgctggaa acagcatagc aagtttaaat aaggctagtc cgttatcaac
      961 tttgctggaa acagcaaagt ggcaccgagt cggtgctttt tttgcctacc tggagcctga
     1021 gagttgttca ataaaataaa aatgtttcgt ttttttgctt tcgccagtat ttattatttt
     1081 tcatcaatat gtattcaatt tggtatgtat ttacgcgtgg cttgctgttc ttcgcgttca
     1141 aaatctccag ctccattttg ctttcggtgc gcttgcaatc agtactgtcc aaaatcgaaa
     1201 atcgccgaac cgtagtgtga ccgtgcgggg ctctgcgaaa ataaactttt ttaggtatat
     1261 ggccacacac ggggaaagca cagtggatta tatgtattaa tatattatgc aggttttcat
     1321 tacttatcca gatgtaagcc cacttaaagc gatttaacaa ttatttgccg aaagagtata
     1381 aacaaatttc acataaaaat ggattaagaa aagcttgtgt aagattatgc gcagcgttgc
     1441 cagatagctc catttaaaac acttcaaaaa caataagttt agaaaatata tacataaata
     1501 gcagtcgttg ccgcaacgct caacacatca cacttttaaa acacccttta cctacacaga
     1561 attacttttt aaatttccag tcaagctgcg agtttcaaaa ttatagccgg tagagaagac
     1621 agtgctattt caaaagcaaa ctaacaaggg tcttaaattt caaaacacca atcctaacaa
     1681 gccttggact tttgtaagtt tagatcaaag gtggcattgc attcaatgtc atggtaagaa
     1741 gtaggtcgtc taggtagaaa tcctcattca gccggtcaag tcagtacgag aaaggtctca
     1801 atttgaaatt gtcttaaaaa tattttattg ttttgtactg tggtgagttt aaacgaaaaa
     1861 cacaaaaaaa aagtgataca cagaaatcat aaaaaatttt aatacaaggt attcgtacgt
     1921 atcaaaaaca tttcggcaca attttttttc tctgtactaa agtgttacga acactacggt
     1981 attttttagt gattttcaac ggacaccgaa ggtatataaa cagcgttcgc gaacggtcgc
     2041 cttcaaaacc aattgacatt tgcagcagca agtacaagta gaaagtaaag cgcaatcagc
     2101 gaaaaattta tacttaattg ttggtgatta aagtacaatt aaaagaacat tctcgaaagt
     2161 cacaagaaac gtaagttttt aactcgctgt taccaattag taataagagc aacaagacgt
     2221 tgagtaattt caagaaaaac tgcatttcaa ggtctttgtt cggccatttt ttttttattc
     2281 aacgctctac gtaattacaa aataagaaat tggcagccac gcatcttgtt ttcccaatga
     2341 attggcatca aaacgcaaac aaatctataa ataaaacttg cgtgttgatt ttcgccaaga
     2401 tttattggca aattgtgaaa ttcgcagtga cgcatttgaa aattcgagaa atcacgaacg
     2461 cactcgagca tttgtgtgca tgttattagt tagttgttta gttaattgaa gtattttacc
     2521 aacgaaatcc acttattttt agctgaaata gagtaggttg cttaaacaaa gccacgtctg
     2581 aaaatttctt attgcttgta gttgtgacgt caccatatac acacaaaata atgtgtatgc
     2641 atgcgtttca gctgtgtata tatacatgca cacactcgca ttatgaaaac gatgacgagc
     2701 aacggaacag gtttctcaac tacctttgtt cctgtttctt cgctttcctt tgttccaata
     2761 ttcgtagagg gttaataggg gtttctcaac aaagttggcg tcgataaata agtttcccat
     2821 ttttattccc cagccaggaa gttagtttca atagttttgt aatttcaacg aaactcattt
     2881 gatttcgtac taattttcca catctctatt ttctgcccgc agaataatcc aactgaatag
     2941 ggaattggga acttaaaaaa aaaaatcaaa atgagcggta ccgatcgcag cccccccaag
     3001 aagaagcgca aggtggagtt cggatccggt ggcggcggaa gtggaggtgg aggctcgatg
     3061 gtgtccaagg gcgaggagct gatcaaggag aacatgcaca tgaagctgta catggagggc
     3121 accgtggaca accaccactt caagtgcacc tccgagggcg agggcaagcc ctacgagggc
     3181 acccagacca tgcgcatcaa ggtggtggag ggcggccccc tgcccttcgc cttcgacatc
     3241 ctggccacct ccttcctgta cggctccaag accttcatca accacaccca gggcatcccc
     3301 gacttcttca agcagtcctt ccccgagggc ttcacctggg agcgcgtgac cacctacgag
     3361 gacggcggcg tgctgaccgc cacccaggac acctccctgc aagacggctg cctgatctac
     3421 aacgtgaaga tccgcggcgt gaacttcacc tccaacggcc ccgtgatgca gaagaagacc
     3481 ctgggctggg aggccttcac cgagaccctg taccccgccg acggcggcct ggagggccgc
     3541 aacgacatgg ccctgaagct ggtgggcggc tcccacctga tcgccaacgc caagaccacc
     3601 taccgctcca agaagcccgc caagaacctg aagatgcccg gcgtgtacta cgtggactac
     3661 cgcctggagc gcatcaagga ggccaacaac gagacctacg tggagcagca cgaggtggcc
     3721 gtggcccgct actgcgacct gccctccaag ctgggccaca agctgaacta atctagaatt
     3781 aattaactaa gccagtcggc aatcggacgc cttcgaaaca tgcaacacta atgtttaatt
     3841 cagatttcag cagagacaag ctaaaacacc gacgagttgt aatcatttct gtgcgccagc
     3901 atatatttct tatatacaac gtaatacata attatgtaat tctagcatct ccccaacact
     3961 cacatacaaa caaacaaaaa atacaaacac acaaaacgta tttacccgca cgcatccttg
     4021 gcgaggttga gtatgaaaca aaaacaaaac ttaatttaga gcaaagtaat tacacgaata
     4081 aatttaataa aaaaaaacta taataaaaac gccaatcatg ttatttccaa aaaaaaaaaa
     4141 aaaaaaacta gacgagatgt ttgcgcttgt gtcgatgaat gtaaagaatt gcaactacat
     4201 agatggtaat taatttcagc tgtaagaagt acaagttttt cataattgtg agaaaagtgc
     4261 agtagtatcg cttaaagttc agttttaatc agggcattta gcagctcgaa cccaatgtat
     4321 ttgtaagccc aataaacgtg taaatgccta gagcaaaata tcacaactgt agcttttcat
     4381 ccgaaagata ataatgaata gactgacctg acgtttttca aaatgcgact agtggcctaa
     4441 ttctagtatg tatgtaagtt aataaaaccc atttttgcgg aaagtagata aaaaaaacat
     4501 tttttttttt tactgcactg gatatcattg aacttatctg atcagtttta aatttacttc
     4561 gatccaaggg tatttgatgt accaggttct ttcgattacc tctcactcaa aatgacattc
     4621 cactcaaagt cagcgctgtt tgcctccttc tctgtccaca gaaatatcgc cgtctctttc
     4681 gccgctgcgt ccgctatctc tttcgccacc gtttgtagcg ttacgtagcg tcaatgtccg
     4741 ccttcagttg cattttgtca gcggtttcgt gacgaagctc caagcggttt acgccatcaa
     4801 ttaaacacaa agtgctgtgc caaaactcct ctcgcttctt atttttgttt gttttttgag
     4861 tgattggggt ggtgattggt tttgggtggg taagcagggg aaagtgtgaa aaatcccggc
     4921 aatgggccaa gaggatcagg agctattaat tcgcggaggc agcaaacacc catctgccga
     4981 gcatctgaac aatgtgagta gtacatgtgc atacatctta agttcacttg atctatagga
     5041 actgcgattg caacatcaaa ttgtctgcgg cgtgagaact gcgacccaca aaaatcccaa
     5101 accgcaattg cacaaacaaa tagtgacacg aaacagatta ttctggtagc tgttctcgct
     5161 atataagaca atttttgaga tcatatcatg atcaagacat ctaaaggcat tcattttcga
     5221 ctatattctt ttttacaaaa aatataacaa ccagatattt taagctgatc ctagatgcac
     5281 aaaaaataaa taaaagtata aacctacttc gtaggatact tcggggtact ttttgttcgg
     5341 ggttagatga gcataacgct tgtagttgat atttgagatc ccctatcatt gcagggtgac
     5401 agcggagcgg cttcgcagag ctgcattaac cagggcttcg ggcaggccaa aaactacggc
     5461 acgctccggc cacccagtcc gccggaggac tccggttcag ggagcggcca actagccgag
     5521 aacctcacct atgcctggca caatatggac atctttgggg cggtcaatca gccgggctcc
     5581 ggatggcggc agctggtcaa ccggacacgc ggactattct gcaacgagcg acacataccg
     5641 gcgcccagga aacatttgct caagaacggt gagtttctat tcgcagtcgg ctgatctgtg
     5701 tgaaatctta ataaagggtc caattaccaa tttgaaactc agtttgcggc gtggcctatc
     5761 cgggcgaact tttggccgtg atgggcagtt ccggtgccgg aaagacgacc ctgctgaatg
     5821 cccttgcctt tcgatcgccg cagggcatcc aagtatcgcc atccgggatg cgactgctca
     5881 atggccaacc tgtggacgcc aaggagatgc aggccaggtg cgcctatgtc cagcaggatg
     5941 acctctttat cggctcccta acggccaggg aacacctgat tttccaagcc atggtgcgga
     6001 tgccacgaca tctgacctat cggcagcgag tggcccgcgt ggatcaggtg atccaggagc
     6061 tttcgctcag caaatgtcag cacacgatca tcggtgtgcc cggcagggtg aaaggtctgt
     6121 ccggcggaga aaggaagcgt ctggcattcg cctccgaggc tctaaccgat ccgccgcttc
     6181 tgatctgcga tgagcccacc tccggactgg actcctttac cgcccacagc gtcgtccagg
     6241 tgctgaagaa gctgtcgcag aagggcaaga ccgtcatcct gaccattcat cagccgtctt
     6301 ccgagctgtt tgagctcttt gacaagatcc ttctgatggc cgagggcagg gtagctttct
     6361 tgggcactcc cagcgaagcc gtcgacttct tttcctagtg agttcgatgt gtttattaag
     6421 ggtatctagt attacataac atctcaactc ctatccagcg tgggtgccca gtgtcctacc
     6481 aactacaatc cggcggactt ttacgtacag gtgttggccg ttgtgcccgg acgggagatc
     6541 gagtcccgtg atcggatcgc caagatatgc gacaattttg ccattagcaa agtagcccgg
     6601 gatatggagc agttgttggc caccaaaaat ctggagaagc cactggagca gccggagaat
     6661 gggtacacct acaaggccac ctggttcatg cagttccggg cggtcctgtg gcgatcctgg
     6721 ctgtcggtgc tcaaggaacc actcctcgta aaagtgcgac ttattcagac aacggtgagt
     6781 ggttccagtg gaaacaaatg atataacgct tacaattctt ggaaacaaat tcgctagatt
     6841 ttagatagaa ttgcctgatt ccacaccctt cttagttttt ttcaatgaga tgtatagttt
     6901 atagttttgc agaagataaa taaatttcat ttaactcgcg aatattaatg agatgcgagt
     6961 aacattttaa tttgcagatg gttgccatct tgattggcct catctttttg ggccaacaac
     7021 tcacgcaagt gggtgtgatg aatatcaacg gagccatctt cctcttcctg accaacatga
     7081 cctttcaaaa cgtctttgcc acgataaatg taagtcatgt ttagaataca tttgcatttc
     7141 aataatttac taactttcta atgaatcgat tcgatttagg tgttcacctc agagctgcca
     7201 gtttttatga gggaggcccg aagtcgactt tatcgctgtg acacatactt tctgggcaaa
     7261 acgattgccg aattgccgct ttttctcaca gtgccactgg tcttcacggc gattgcctat
     7321 ccgatgatcg gactgcgggc cggagtgctg cacttcttca actgcctggc gctggtcact
     7381 ctggtggcca atgtgtcaac gtccttcgga tatctaatat cctgcgccag ctcctcgacc
     7441 tcgatggcgc tgtctgtggg tccgccggtt atcataccat tcctgctctt tggcggcttc
     7501 ttcttgaact cgggctcggt gccagtatac ctcaaatggt tgtcgtacct ctcatggttc
     7561 cgttacgcca acgagggtct gctgattaac caatgggcgg acgtggagcc gggcgaaatt
     7621 agctgcacat cgtcgaacac cacgtgcccc agttcgggca aggtcatcct ggagacgctt
     7681 aacttctccg ccgccgatct gccgctggac tacgtgggtc tggccattct catcgtgagc
     7741 ttccgggtgc tcgcatatct ggctctaaga cttcgggccc gacgcaagga gtagccgaca
     7801 tatatccgaa ataactgctt gttttttttt tttaccatta ttaccatcgt gtttactgtt
     7861 tattgccccc tcaaaaagct aatgtaatta tatttgtgcc aataaaaaca agatatgacc
     7921 tatagaatac aagtatttcc ccttcgaaca tccccacaag tagactttgg atttgtcttc
     7981 taaccaaaag acttacacac ctgcatacct tacatcaaaa actcgtttat cgctacataa
     8041 aacaccggga tatatttttt atatacatac ttttcaaatc gcgcgccctc ttcataattc
     8101 acctccacca caccacgttt cgtagttgct ctttcgctgt ctcccacccg ctctccgcaa
     8161 cacattcacc ttttgttcga cgaccttgga gcgactgtcg ttagttccgc gcgattcggt
     8221 tcgctcaaat ggttccgagt ggttcatttc gtctcaatag aaattagtaa taaatatttg
     8281 tatgtacaat ttatttgctc caatatattt gtatatattt ccctcacagc tatatttatt
     8341 ctaatttaat attatgactt tttaaggtaa ttttttgtga cctgttcgga gtgattagcg
     8401 ttacaatttg aactgaaagt gacatccagt gtttgttcct tgtgtagatg catctcaaaa
     8461 aaatggtggg cataatagtg ttgtttatat atatcaaaaa taacaactat aataataaga
     8521 atacatttaa tttagaaaat gcttggattt cactggaact agaattaatt cggctgctgc
     8581 tctaaacgac gcatttcgta ctccaaagta cgaatttttt ccctcaagct cttattttca
     8641 ttaaacaatg aacaggacct aacgcacagt cacgttattg tttacataaa tgattttttt
     8701 tactattcaa acttactctg tttgtgtact cccactggta tagccttctt ttatcttttc
     8761 tggttcaggc tctatcactt tactaggtac ggcatctgcg ttgagtcgcc tccttttaaa
     8821 tgtctgacct tttgcaggtg cagccttcca ctgcgaatca ttaaagtggg tatcacaaat
     8881 ttgggagttt tcaccaaggc tgcacccaag gctctgctcc cacaattttc tcttaatagc
     8941 acacttcggc acgtgaatta attttactcc agtcacagct ttgcagcaaa atttgcaata
     9001 tttcattttt ttttattcca cgtaagggtt aatgttttca aaaaaaaatt cgtccgcaca
     9061 caacctttcc tctcaacaag caaacgtgca ctgaatttaa gtgtatactt cggtaagctt
     9121 cggctatcga cgggaccacc ttatgttatt tcatcatggg ccagacccac gtagtccagc
     9181 ggcagatcgg cggcggagaa gttaagcgtc tccaggatga ccttgcccga actggggcac
     9241 gtggtgttcg acgatgtgca gctaatttcg cccggctcca cgtccgccca ttggttaatc
     9301 agcagaccct cgttggcgta acggaaccat gagaggtacg acaaccattt gaggtatact
     9361 ggcaccgagc ccgagttcaa gaagaaggcg tttttccata ggctccgccc ccctgacgag
     9421 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     9481 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     9541 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     9601 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     9661 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     9721 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     9781 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     9841 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     9901 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     9961 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
    10021 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
    10081 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
    10141 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
    10201 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
    10261 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
    10321 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
    10381 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
    10441 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
    10501 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
    10561 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
    10621 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
    10681 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
    10741 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
    10801 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
    10861 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
    10921 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    10981 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
    11041 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    11101 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
    11161 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt
    11221 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
    11281 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
    11341 tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatc
    11401 gacatgcccg ccgtgaccgt cgagaacccg ctgacgctgc cccgcgtatc cgcacccgcc
    11461 gacgccgtcg cacgtcccgt gctcaccgtg accaccgcgc ccagcggttt cgagggcgag
    11521 ggcttcccgg tgcgccgcgc gttcgccggg atcaactacc gccacctcga cccgttcatc
    11581 atgatggacc agatgggtga ggtggagtac gcgcccgggg agcccaaggg cacgccctgg
    11641 cacccgcacc gcggcttcga gaccgtgacc tacatcgtcg atatgcggtg tgaaataccg
    11701 caccgaatcg cgcggaacta acgacagtcg ctccaaggtc gtcgaacaaa aggtgaatgt
    11761 gttgcggaga gcgggtggga gacagcgaaa gagcaactac gaaacgtggt gtggtggagg
    11821 tgaattatga agagggcgcg cgatttgaaa agtatgtata taaaaaatat atcccggtgt
    11881 tttatgtagc gataaacgag tttttgatgt aaggtatgca ggtgtgtaag tcttttggtt
    11941 agaagacaaa tccaaagtct acttgtgggg atgttcgaag gggaaatact tgtattctat
    12001 aggtcatatc ttgtttttat tggcacaaat ataattacat tagctttttg agggggcaat
    12061 aaacagtaaa cacgatggta ataatggtaa aaaaaaaaaa caagcagtta tttcggatat
    12121 atgtcggcta ctccttgcgt cgggcccgaa gtcttagagc cagatatgcg agcacccgga
    12181 agctcacgat gagaatggcc agac
//
