LOCUS       sequence-334668-       12222 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gRNA-marker vector contain a U6:3 promoter, gRNA scaffold, and a 
            Ubi-mTagBFP-NLS marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12222)
  AUTHORS   Allen SE, Koreman GT, Sarkar A, Wang B, Wolfner MF, Han C
  TITLE     Versatile CRISPR/Cas9-mediated mosaic analysis by gRNA-induced 
            crossing-over for unmodified genomes.
  JOURNAL   PLoS Biol. 2021 Jan 14;19(1):e3001061. doi: 
            10.1371/journal.pbio.3001061. eCollection 2021 Jan.
  PUBMED    33444322
REFERENCE   2  (bases 1 to 12222)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2021-01-14"; volume: "19(1):e3001061. doi"; pages: " 
            10.1371/journal.pbio.3001061. eCollection 2021 Jan"
COMMENT     Sequence Label: pAC-U63-tgRNA-nlsBFP
FEATURES             Location/Qualifiers
     source          1..12222
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(1..31,32..233)
                     /label=P element 3' end
                     /note="P element 3' end"
     CDS             3013..3033
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(4470..4488)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(4940..5011,5413..5686,5761..6415,6477..6792,6996..7127,
                     7198..7812)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(8591..9145,9146..9176)
                     /label=P element 5' end
     rep_origin      complement(9411..9999)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9491..9510)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10170..10961,10962..11030))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10793..10812
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11031..11135)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11203..11221
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(11259..11281)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     11381..11400
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    11610..11679
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
       61 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
      121 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
      181 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttg
      241 gctgcagtcg acgctagcat atgtttgctc acctgtgatt gctcctactc aaatacaaaa
      301 acatcaaatt ttctgtcaat aaagcatatt tatttatatt tattttacag gaaagaattc
      361 cttttaaagt gtattttaac ctataatgaa aaacgattaa aaaaaataca taaaataatt
      421 cgaaaatttt tgaatagccc aggttgataa aaattcattt catacgtttt ataacttatg
      481 cccctaagta ttttttgacc atagtgtttc aattctacat taattttaca gagtagaatg
      541 aaacgccacc tactcagcca agaggcgaaa aggttagctc gccaagcaga gagggcgcca
      601 gtgctcacta ctttttataa ttctcaactt ctttttccag actcagttcg tatatataga
      661 cctattttca atttaacgtc ggggctttga gtgtgtgtag acatcaagca tcggtggttc
      721 agtggtagaa tgctcgcctg ccacgcgggc ggcccgggtt cgattcccgg ccgatgcaca
      781 aggaagagcg aggctcttct tgtgtttaag agctatgctg gaaacagcat agcaagttta
      841 aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttgcct
      901 acctggagcc tgagagttgt tcaataaaat aaaaatgttt cgtttttttg ctttcgccag
      961 tatttattat ttttcatcaa tatgtattca atttggtatg tatttagtaa ttgtaatata
     1021 tagacaatgg ttttccgttg acgtacatac atctgacgtg tgtttattta gacataatag
     1081 ttatgttttc acatcttttt aatgttcgct taatgcgtat gcattctagt ggccatggct
     1141 tgctgttctt cgcgttcaaa atctccagct ccattttgct ttcggtgcgc ttgcaatcag
     1201 tactgtccaa aatcgaaaat cgccgaaccg tagtgtgacc gtgcggggct ctgcgaaaat
     1261 aaactttttt aggtatatgg ccacacacgg ggaaagcaca gtggattata tgtattaata
     1321 tattatgcag gttttcatta cttatccaga tgtaagccca cttaaagcga tttaacaatt
     1381 atttgccgaa agagtataaa caaatttcac ataaaaatgg attaagaaaa gcttgtgtaa
     1441 gattatgcgc agcgttgcca gatagctcca tttaaaacac ttcaaaaaca ataagtttag
     1501 aaaatatata cataaatagc agtcgttgcc gcaacgctca acacatcaca cttttaaaac
     1561 accctttacc tacacagaat tactttttaa atttccagtc aagctgcgag tttcaaaatt
     1621 atagccggta gagaagacag tgctatttca aaagcaaact aacaagggtc ttaaatttca
     1681 aaacaccaat cctaacaagc cttggacttt tgtaagttta gatcaaaggt ggcattgcat
     1741 tcaatgtcat ggtaagaagt aggtcgtcta ggtagaaatc ctcattcagc cggtcaagtc
     1801 agtacgagaa aggtctcaat ttgaaattgt cttaaaaata ttttattgtt ttgtactgtg
     1861 gtgagtttaa acgaaaaaca caaaaaaaaa gtgatacaca gaaatcataa aaaattttaa
     1921 tacaaggtat tcgtacgtat caaaaacatt tcggcacaat tttttttctc tgtactaaag
     1981 tgttacgaac actacggtat tttttagtga ttttcaacgg acaccgaagg tatataaaca
     2041 gcgttcgcga acggtcgcct tcaaaaccaa ttgacatttg cagcagcaag tacaagtaga
     2101 aagtaaagcg caatcagcga aaaatttata cttaattgtt ggtgattaaa gtacaattaa
     2161 aagaacattc tcgaaagtca caagaaacgt aagtttttaa ctcgctgtta ccaattagta
     2221 ataagagcaa caagacgttg agtaatttca agaaaaactg catttcaagg tctttgttcg
     2281 gccatttttt ttttattcaa cgctctacgt aattacaaaa taagaaattg gcagccacgc
     2341 atcttgtttt cccaatgaat tggcatcaaa acgcaaacaa atctataaat aaaacttgcg
     2401 tgttgatttt cgccaagatt tattggcaaa ttgtgaaatt cgcagtgacg catttgaaaa
     2461 ttcgagaaat cacgaacgca ctcgagcatt tgtgtgcatg ttattagtta gttgtttagt
     2521 taattgaagt attttaccaa cgaaatccac ttatttttag ctgaaataga gtaggttgct
     2581 taaacaaagc cacgtctgaa aatttcttat tgcttgtagt tgtgacgtca ccatatacac
     2641 acaaaataat gtgtatgcat gcgtttcagc tgtgtatata tacatgcaca cactcgcatt
     2701 atgaaaacga tgacgagcaa cggaacaggt ttctcaacta cctttgttcc tgtttcttcg
     2761 ctttcctttg ttccaatatt cgtagagggt taataggggt ttctcaacaa agttggcgtc
     2821 gataaataag tttcccattt ttattcccca gccaggaagt tagtttcaat agttttgtaa
     2881 tttcaacgaa actcatttga tttcgtacta attttccaca tctctatttt ctgcccgcag
     2941 aataatccaa ctgaataggg aattgggaac ttaaaaaaaa aaatcaaaat gagcggtacc
     3001 gatcgcagcc cccccaagaa gaagcgcaag gtggagttcg gatccggtgg cggcggaagt
     3061 ggaggtggag gctcgatggt gtccaagggc gaggagctga tcaaggagaa catgcacatg
     3121 aagctgtaca tggagggcac cgtggacaac caccacttca agtgcacctc cgagggcgag
     3181 ggcaagccct acgagggcac ccagaccatg cgcatcaagg tggtggaggg cggccccctg
     3241 cccttcgcct tcgacatcct ggccacctcc ttcctgtacg gctccaagac cttcatcaac
     3301 cacacccagg gcatccccga cttcttcaag cagtccttcc ccgagggctt cacctgggag
     3361 cgcgtgacca cctacgagga cggcggcgtg ctgaccgcca cccaggacac ctccctgcaa
     3421 gacggctgcc tgatctacaa cgtgaagatc cgcggcgtga acttcacctc caacggcccc
     3481 gtgatgcaga agaagaccct gggctgggag gccttcaccg agaccctgta ccccgccgac
     3541 ggcggcctgg agggccgcaa cgacatggcc ctgaagctgg tgggcggctc ccacctgatc
     3601 gccaacgcca agaccaccta ccgctccaag aagcccgcca agaacctgaa gatgcccggc
     3661 gtgtactacg tggactaccg cctggagcgc atcaaggagg ccaacaacga gacctacgtg
     3721 gagcagcacg aggtggccgt ggcccgctac tgcgacctgc cctccaagct gggccacaag
     3781 ctgaactaat ctagaattaa ttaactaagc cagtcggcaa tcggacgcct tcgaaacatg
     3841 caacactaat gtttaattca gatttcagca gagacaagct aaaacaccga cgagttgtaa
     3901 tcatttctgt gcgccagcat atatttctta tatacaacgt aatacataat tatgtaattc
     3961 tagcatctcc ccaacactca catacaaaca aacaaaaaat acaaacacac aaaacgtatt
     4021 tacccgcacg catccttggc gaggttgagt atgaaacaaa aacaaaactt aatttagagc
     4081 aaagtaatta cacgaataaa tttaataaaa aaaaactata ataaaaacgc caatcatgtt
     4141 atttccaaaa aaaaaaaaaa aaaaactaga cgagatgttt gcgcttgtgt cgatgaatgt
     4201 aaagaattgc aactacatag atggtaatta atttcagctg taagaagtac aagtttttca
     4261 taattgtgag aaaagtgcag tagtatcgct taaagttcag ttttaatcag ggcatttagc
     4321 agctcgaacc caatgtattt gtaagcccaa taaacgtgta aatgcctaga gcaaaatatc
     4381 acaactgtag cttttcatcc gaaagataat aatgaataga ctgacctgac gtttttcaaa
     4441 atgcgactag tggcctaatt ctagtatgta tgtaagttaa taaaacccat ttttgcggaa
     4501 agtagataaa aaaaacattt ttttttttta ctgcactgga tatcattgaa cttatctgat
     4561 cagttttaaa tttacttcga tccaagggta tttgatgtac caggttcttt cgattacctc
     4621 tcactcaaaa tgacattcca ctcaaagtca gcgctgtttg cctccttctc tgtccacaga
     4681 aatatcgccg tctctttcgc cgctgcgtcc gctatctctt tcgccaccgt ttgtagcgtt
     4741 acgtagcgtc aatgtccgcc ttcagttgca ttttgtcagc ggtttcgtga cgaagctcca
     4801 agcggtttac gccatcaatt aaacacaaag tgctgtgcca aaactcctct cgcttcttat
     4861 ttttgtttgt tttttgagtg attggggtgg tgattggttt tgggtgggta agcaggggaa
     4921 agtgtgaaaa atcccggcaa tgggccaaga ggatcaggag ctattaattc gcggaggcag
     4981 caaacaccca tctgccgagc atctgaacaa tgtgagtagt acatgtgcat acatcttaag
     5041 ttcacttgat ctataggaac tgcgattgca acatcaaatt gtctgcggcg tgagaactgc
     5101 gacccacaaa aatcccaaac cgcaattgca caaacaaata gtgacacgaa acagattatt
     5161 ctggtagctg ttctcgctat ataagacaat ttttgagatc atatcatgat caagacatct
     5221 aaaggcattc attttcgact atattctttt ttacaaaaaa tataacaacc agatatttta
     5281 agctgatcct agatgcacaa aaaataaata aaagtataaa cctacttcgt aggatacttc
     5341 ggggtacttt ttgttcgggg ttagatgagc ataacgcttg tagttgatat ttgagatccc
     5401 ctatcattgc agggtgacag cggagcggct tcgcagagct gcattaacca gggcttcggg
     5461 caggccaaaa actacggcac gctccggcca cccagtccgc cggaggactc cggttcaggg
     5521 agcggccaac tagccgagaa cctcacctat gcctggcaca atatggacat ctttggggcg
     5581 gtcaatcagc cgggctccgg atggcggcag ctggtcaacc ggacacgcgg actattctgc
     5641 aacgagcgac acataccggc gcccaggaaa catttgctca agaacggtga gtttctattc
     5701 gcagtcggct gatctgtgtg aaatcttaat aaagggtcca attaccaatt tgaaactcag
     5761 tttgcggcgt ggcctatccg ggcgaacttt tggccgtgat gggcagttcc ggtgccggaa
     5821 agacgaccct gctgaatgcc cttgcctttc gatcgccgca gggcatccaa gtatcgccat
     5881 ccgggatgcg actgctcaat ggccaacctg tggacgccaa ggagatgcag gccaggtgcg
     5941 cctatgtcca gcaggatgac ctctttatcg gctccctaac ggccagggaa cacctgattt
     6001 tccaagccat ggtgcggatg ccacgacatc tgacctatcg gcagcgagtg gcccgcgtgg
     6061 atcaggtgat ccaggagctt tcgctcagca aatgtcagca cacgatcatc ggtgtgcccg
     6121 gcagggtgaa aggtctgtcc ggcggagaaa ggaagcgtct ggcattcgcc tccgaggctc
     6181 taaccgatcc gccgcttctg atctgcgatg agcccacctc cggactggac tcctttaccg
     6241 cccacagcgt cgtccaggtg ctgaagaagc tgtcgcagaa gggcaagacc gtcatcctga
     6301 ccattcatca gccgtcttcc gagctgtttg agctctttga caagatcctt ctgatggccg
     6361 agggcagggt agctttcttg ggcactccca gcgaagccgt cgacttcttt tcctagtgag
     6421 ttcgatgtgt ttattaaggg tatctagtat tacataacat ctcaactcct atccagcgtg
     6481 ggtgcccagt gtcctaccaa ctacaatccg gcggactttt acgtacaggt gttggccgtt
     6541 gtgcccggac gggagatcga gtcccgtgat cggatcgcca agatatgcga caattttgcc
     6601 attagcaaag tagcccggga tatggagcag ttgttggcca ccaaaaatct ggagaagcca
     6661 ctggagcagc cggagaatgg gtacacctac aaggccacct ggttcatgca gttccgggcg
     6721 gtcctgtggc gatcctggct gtcggtgctc aaggaaccac tcctcgtaaa agtgcgactt
     6781 attcagacaa cggtgagtgg ttccagtgga aacaaatgat ataacgctta caattcttgg
     6841 aaacaaattc gctagatttt agatagaatt gcctgattcc acacccttct tagttttttt
     6901 caatgagatg tatagtttat agttttgcag aagataaata aatttcattt aactcgcgaa
     6961 tattaatgag atgcgagtaa cattttaatt tgcagatggt tgccatcttg attggcctca
     7021 tctttttggg ccaacaactc acgcaagtgg gtgtgatgaa tatcaacgga gccatcttcc
     7081 tcttcctgac caacatgacc tttcaaaacg tctttgccac gataaatgta agtcatgttt
     7141 agaatacatt tgcatttcaa taatttacta actttctaat gaatcgattc gatttaggtg
     7201 ttcacctcag agctgccagt ttttatgagg gaggcccgaa gtcgacttta tcgctgtgac
     7261 acatactttc tgggcaaaac gattgccgaa ttgccgcttt ttctcacagt gccactggtc
     7321 ttcacggcga ttgcctatcc gatgatcgga ctgcgggccg gagtgctgca cttcttcaac
     7381 tgcctggcgc tggtcactct ggtggccaat gtgtcaacgt ccttcggata tctaatatcc
     7441 tgcgccagct cctcgacctc gatggcgctg tctgtgggtc cgccggttat cataccattc
     7501 ctgctctttg gcggcttctt cttgaactcg ggctcggtgc cagtatacct caaatggttg
     7561 tcgtacctct catggttccg ttacgccaac gagggtctgc tgattaacca atgggcggac
     7621 gtggagccgg gcgaaattag ctgcacatcg tcgaacacca cgtgccccag ttcgggcaag
     7681 gtcatcctgg agacgcttaa cttctccgcc gccgatctgc cgctggacta cgtgggtctg
     7741 gccattctca tcgtgagctt ccgggtgctc gcatatctgg ctctaagact tcgggcccga
     7801 cgcaaggagt agccgacata tatccgaaat aactgcttgt tttttttttt taccattatt
     7861 accatcgtgt ttactgttta ttgccccctc aaaaagctaa tgtaattata tttgtgccaa
     7921 taaaaacaag atatgaccta tagaatacaa gtatttcccc ttcgaacatc cccacaagta
     7981 gactttggat ttgtcttcta accaaaagac ttacacacct gcatacctta catcaaaaac
     8041 tcgtttatcg ctacataaaa caccgggata tattttttat atacatactt ttcaaatcgc
     8101 gcgccctctt cataattcac ctccaccaca ccacgtttcg tagttgctct ttcgctgtct
     8161 cccacccgct ctccgcaaca cattcacctt ttgttcgacg accttggagc gactgtcgtt
     8221 agttccgcgc gattcggttc gctcaaatgg ttccgagtgg ttcatttcgt ctcaatagaa
     8281 attagtaata aatatttgta tgtacaattt atttgctcca atatatttgt atatatttcc
     8341 ctcacagcta tatttattct aatttaatat tatgactttt taaggtaatt ttttgtgacc
     8401 tgttcggagt gattagcgtt acaatttgaa ctgaaagtga catccagtgt ttgttccttg
     8461 tgtagatgca tctcaaaaaa atggtgggca taatagtgtt gtttatatat atcaaaaata
     8521 acaactataa taataagaat acatttaatt tagaaaatgc ttggatttca ctggaactag
     8581 aattaattcg gctgctgctc taaacgacgc atttcgtact ccaaagtacg aattttttcc
     8641 ctcaagctct tattttcatt aaacaatgaa caggacctaa cgcacagtca cgttattgtt
     8701 tacataaatg atttttttta ctattcaaac ttactctgtt tgtgtactcc cactggtata
     8761 gccttctttt atcttttctg gttcaggctc tatcacttta ctaggtacgg catctgcgtt
     8821 gagtcgcctc cttttaaatg tctgaccttt tgcaggtgca gccttccact gcgaatcatt
     8881 aaagtgggta tcacaaattt gggagttttc accaaggctg cacccaaggc tctgctccca
     8941 caattttctc ttaatagcac acttcggcac gtgaattaat tttactccag tcacagcttt
     9001 gcagcaaaat ttgcaatatt tcattttttt ttattccacg taagggttaa tgttttcaaa
     9061 aaaaaattcg tccgcacaca acctttcctc tcaacaagca aacgtgcact gaatttaagt
     9121 gtatacttcg gtaagcttcg gctatcgacg ggaccacctt atgttatttc atcatgggcc
     9181 agacccacgt agtccagcgg cagatcggcg gcggagaagt taagcgtctc caggatgacc
     9241 ttgcccgaac tggggcacgt ggtgttcgac gatgtgcagc taatttcgcc cggctccacg
     9301 tccgcccatt ggttaatcag cagaccctcg ttggcgtaac ggaaccatga gaggtacgac
     9361 aaccatttga ggtatactgg caccgagccc gagttcaaga agaaggcgtt tttccatagg
     9421 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     9481 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     9541 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     9601 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     9661 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     9721 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     9781 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     9841 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     9901 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     9961 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
    10021 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
    10081 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
    10141 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
    10201 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
    10261 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
    10321 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
    10381 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
    10441 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
    10501 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
    10561 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
    10621 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
    10681 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
    10741 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
    10801 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
    10861 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
    10921 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
    10981 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
    11041 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
    11101 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
    11161 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
    11221 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
    11281 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
    11341 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
    11401 ctgagagtgc accatatcga catgcccgcc gtgaccgtcg agaacccgct gacgctgccc
    11461 cgcgtatccg cacccgccga cgccgtcgca cgtcccgtgc tcaccgtgac caccgcgccc
    11521 agcggtttcg agggcgaggg cttcccggtg cgccgcgcgt tcgccgggat caactaccgc
    11581 cacctcgacc cgttcatcat gatggaccag atgggtgagg tggagtacgc gcccggggag
    11641 cccaagggca cgccctggca cccgcaccgc ggcttcgaga ccgtgaccta catcgtcgat
    11701 atgcggtgtg aaataccgca ccgaatcgcg cggaactaac gacagtcgct ccaaggtcgt
    11761 cgaacaaaag gtgaatgtgt tgcggagagc gggtgggaga cagcgaaaga gcaactacga
    11821 aacgtggtgt ggtggaggtg aattatgaag agggcgcgcg atttgaaaag tatgtatata
    11881 aaaaatatat cccggtgttt tatgtagcga taaacgagtt tttgatgtaa ggtatgcagg
    11941 tgtgtaagtc ttttggttag aagacaaatc caaagtctac ttgtggggat gttcgaaggg
    12001 gaaatacttg tattctatag gtcatatctt gtttttattg gcacaaatat aattacatta
    12061 gctttttgag ggggcaataa acagtaaaca cgatggtaat aatggtaaaa aaaaaaaaca
    12121 agcagttatt tcggatatat gtcggctact ccttgcgtcg ggcccgaagt cttagagcca
    12181 gatatgcgag cacccggaag ctcacgatga gaatggccag ac
//
