LOCUS       sequence-334673-        4371 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4371)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4371)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC26A11_STOP
FEATURES             Location/Qualifiers
     source          1..4371
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1928..2027)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2044..2063)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2045..2063)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2068..2084)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2068..2084)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2186..2205)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2197..3006
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2264..2283)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3153..3741
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3642..3661
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3895..3912
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4071..4098)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4190..4276)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4325..4347
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4339..4356
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4340..4356
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgcc cagctccgtg
      121 accgccctgg gacaggcacg gtctagcgga ccaggaatgg caccaagcgc ctgctgttgc
      181 tcccctgccg ccctgcagcg gagactgcca atcctggcat ggctgcctag ctactccctg
      241 cagtggctga agatggactt cgtggcagga ctgtccgtgg gactgaccgc aatccctcag
      301 gccctggcat acgcagaggt ggcaggactg ccacctcagt acggcctgta tagcgccttc
      361 atgggctgtt tcgtgtactt cttcctgggc acctccagag atgtgaccct gggccccaca
      421 gccatcatgt ccctgctggt gtctttctac acatttcacg agcctgccta tgccgtgctg
      481 ctggcctttc tgagcggatg catccagctg gcaatgggcg tgctgaggct gggcttcctg
      541 ctggacttta tctcctaccc agtgatcaag ggctttacct ctgccgcagc agtgactata
      601 ggttttgggc aaatcaagaa tctgctgggc ctgcagaata tccccagacc tttctttctg
      661 caggtgtatc acaccttcct gaggatcgcc gagacaaggg tgggcgacgc cgtgctggga
      721 ctggtgtgca tgctgctgct gctggtgctg aagctgatgc gggatcacgt gccacccgtg
      781 cacccagaga tgcctccagg cgtgaggctg tccaggggac tggtgtgggc agcaaccaca
      841 gcaagaaacg cactggtggt gtctttcgcc gccctggtgg cctacagctt tgaggtgacc
      901 ggctatcagc ccttcatcct gaccggagag acagcagagg gactgccacc tgtgcggatc
      961 ccacccttta gcgtgaccac agccaatggc accatctcct tcacagagat ggtgcaggac
     1021 atgggagcag gactggcagt ggtgcccctg atgggcctgc tggagtctat cgccgtggcc
     1081 aaggcctttg ccagccagaa caattacagg atcgatgcca accaggagct gctggccatc
     1141 ggcctgacca atatgctggg ctccctggtg tcctcttatc cagtgaccgg ctcttttggc
     1201 aggacagcag tgaacgcaca gagcggcgtg tgcaccccag caggaggact ggtgacaggc
     1261 gtgctggtgc tgctgtctct ggattacctg acaagcctgt tctactatat ccctaagagc
     1321 gccctggcag ccgtgatcat catggcagtg gcaccactgt tcgacaccaa gatctttagg
     1381 acactgtggc gggtgaagag actggatctg ctgcccctgt gcgtgacctt cctgctgtgc
     1441 ttttgggagg tgcagtatgg aatcctggca ggcgccctgg tgagcctgct gatgctgctg
     1501 cactccgccg caaggcctga gacaaaggtg tctgagggac cagtgctggt gctgcagcca
     1561 gcatctggac tgagcttccc agcaatggag gccctgaggg aggagatcct gtccagagcc
     1621 ctggaggtgt ctcctccacg ctgcctggtg ctggagtgta cccacgtgtg cagcatcgac
     1681 tacacagtgg tgctgggact gggagagctg ctgcaggatt ttcagaagca gggagtggcc
     1741 ctggccttcg tgggactgca ggtgcctgtg ctgcgcgtgc tgctgtccgc cgacctgaag
     1801 ggcttccagt acttttctac cctggaggag gccgagaagc acctgaggca ggagcctggc
     1861 acacagccat ataatatccg cgaggactcc atcctggatc agaaggtggc cctgctgaag
     1921 gcctaagacc cagctttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt
     1981 tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat
     2041 atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg
     2101 tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa
     2161 ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg
     2221 tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc
     2281 gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca
     2341 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc
     2401 agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact
     2461 cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt ccaggtatta
     2521 gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg
     2581 ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct
     2641 caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt
     2701 aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg
     2761 gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa
     2821 ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc
     2881 atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa
     2941 tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt
     3001 ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg
     3061 gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg
     3121 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     3181 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     3241 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     3301 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     3361 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     3421 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     3481 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     3541 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     3601 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     3661 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     3721 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     3781 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     3841 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     3901 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     3961 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     4021 aacgcaatta atacgcgtac cgctagccag gaagagtttg tagaaacgca aaaaggccat
     4081 ccgtcaggat ggccttctgc ttagtttgat gcctggcagt ttatggcggg cgtcctgccc
     4141 gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac
     4201 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct tccgactgag
     4261 cctttcgttt tatttgatgc ctggcagttc cctactctcg cgttaacgct agcatggatg
     4321 ttttcccagt cacgacgttg taaaacgacg gccagtctta agctcgggcc c
//
