LOCUS       sequence-334674-        4212 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4212)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC22A9_STOP
FEATURES             Location/Qualifiers
     source          1..4212
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1769..1868)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1885..1904)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1886..1904)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1909..1925)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1909..1925)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2027..2046)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2038..2847
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2105..2124)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2994..3582
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3483..3502
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3736..3753
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3912..3939)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4031..4117)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4166..4188
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4180..4197
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4181..4197
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatggc atttcaggac
      121 ctgctgggac acgcaggcga tctgtggaga ttccagatcc tgcagaccgt gtttctgagc
      181 atcttcgccg tggccacata cctgcacttt atgctggaga acttcaccgc ctttatccct
      241 ggccacaggt gctgggtgca catcctggac aacgataccg tgtctgacaa tgatacaggc
      301 gccctgagcc aggacgcact gctgaggatc tccatccccc tggattctaa tatgagacct
      361 gagaagtgtc ggagatttgt gcacccacag tggcagctgc tgcacctgaa cggcaccttc
      421 cctaatacat ctgacgccga tatggagcca tgcgtggacg gctgggtgta tgatcgcatc
      481 agcttcagct ccaccatcgt gacagagtgg gacctggtgt gcgattccca gtctctgacc
      541 tccgtggcca agttcgtgtt tatggcagga atgatggtgg gaggaatcct gggaggacac
      601 ctgtctgacc ggtttggcag gcgcttcgtg ctgagatggt gctacctgca ggtggcaatc
      661 gtgggaacct gtgccgccct ggccccaaca tttctgatct attgcagcct gaggttcctg
      721 tccggcatcg ccgccatgag cctgatcacc aacacaatca tgctgatcgc agagtgggca
      781 acccacaggt tccaggcaat gggcatcaca ctgggcatgt gcccctccgg catcgccttt
      841 atgaccctgg caggactggc cttcgccatc agggactggc acatcctgca gctggtggtg
      901 agcgtgcctt acttcgtgat ctttctgaca tctagctggc tgctggagtc cgccaggtgg
      961 ctgatcatca acaataagcc agaggagggc ctgaaggagc tgaggaaggc agcacaccgc
     1021 agcggaatga agaacgcccg ggataccctg acactggaga tcctgaagtc caccatgaag
     1081 aaggagctgg aggccgccca gaagaagaag ccctctctgt gcgagatgct gcacatgcct
     1141 aatatctgta agaggatcag cctgctgtcc ttcacccgct ttgccaactt catggcctac
     1201 ttcggcctga atctgcacgt gcagcacctg ggcaacaacg tgttcctgct gcagacactg
     1261 ttcggcgccg tgatcctgct ggcaaactgc gtggcaccat gggccctgaa gtatatgaat
     1321 cggagagcct ctcagatgct gctgatgttt ctgctggcca tctgtctgct ggccatcatc
     1381 ttcgtgcctc aggagatgca gaccctgaga gaggtgctgg ccacactggg actgggagca
     1441 tccgccctgg ccaacacact ggcctttgcc cacggcaatg aagtgatccc aaccatcatc
     1501 agggcaaggg caatgggcat caacgccaca ttcgccaata tcgcaggcgc cctggcacca
     1561 ctgatgatga tcctgagcgt gtacagcccc cctctgccat ggatcatcta tggcgtgttc
     1621 ccctttatct ccggcttcgc ctttctgctg ctgcctgaga cccggaacaa gccactgttt
     1681 gacacaatcc aggatgagaa gaatgagcgg aaggacccaa gagagcccaa gcaggaggat
     1741 cccagagtgg aggtgaccca gttctaagac ccagctttct tgtacaaagt tggcattata
     1801 agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc
     1861 attatttgcc atccagctga tatcccctat agtgagtcgt attacatggt catagctgtt
     1921 tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat
     1981 atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg
     2041 agccatattc aacgggaaac gtcgaggccg cgattaaatt ccaacatgga tgctgattta
     2101 tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg
     2161 tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat
     2221 gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc
     2281 atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccgga
     2341 aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg
     2401 ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc
     2461 gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg
     2521 agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat
     2581 aaacttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac
     2641 cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca
     2701 gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta
     2761 cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt
     2821 catttgatgc tcgatgagtt tttctaatca gaattggtta attggttgta acactggcag
     2881 agcattacgc tgacttgacg ggacggcgca agctcatgac caaaatccct taacgtgagt
     2941 tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     3001 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     3061 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     3121 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     3181 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     3241 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     3301 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     3361 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     3421 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     3481 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     3541 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     3601 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     3661 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     3721 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     3781 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     3841 tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta ccgctagcca ggaagagttt
     3901 gtagaaacgc aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag
     3961 tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg
     4021 ctcccggcgg atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa
     4081 aggcccagtc ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc
     4141 gcgttaacgc tagcatggat gttttcccag tcacgacgtt gtaaaacgac ggccagtctt
     4201 aagctcgggc cc
//
