LOCUS       sequence-33468-e         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For mammalian expression of human Elk-1 with an HA tag and a 
            mutation in the R repression domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Lee SM, Vasishtha M, Prywes R
  TITLE     Activation and repression of cellular immediate early genes by serum
            response factor cofactors.
  JOURNAL   J Biol Chem. 2010 Jul 16. 285(29):22036-49.
  PUBMED    20466732
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2010 Jul 16. 285(29):22036-49."
COMMENT     Sequence Label: pCGN-ELK-1 R mut
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     5'UTR           106..145
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             175..201
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             181..207
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 cgtgggttta gcagagctgt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
       61 ttttgacctc catagaagac accgggaccg atccagcctc cgggggatct tggtggcgtg
      121 aaactcccgc acctcttcgg ccagcgcctt gtagaagcgc gtatggcttc tagctatcct
      181 tatgacgtgc ctgactatgc cagcctggga ggaccttcta gaatggaccc atctgtgacg
      241 ctgtggcagt ttctgctgca gctgctgaga gagcaaggca atggccacat catctcctgg
      301 acttcacggg atggtggtga attcaagctg gtggatgcag aggaggtggc ccggctgtgg
      361 ggactacgca agaacaagac caacatgaat tacgacaagc tcagccgggc cttgcggtac
      421 tactatgaca agaacatcat ccgcaaggtg agcggccaga agttcgtcta caagtttgtg
      481 tcctaccctg aggtcgcagg gtgctccact gaggactgcc cgccccagcc agaggtgtct
      541 gttacctcca ccatgccaaa tgtggcccct gctgctatac atgccgcccc aggggacact
      601 gtctctggaa agccaggcac acccaagggt gcaggaatgg caggcccagg cggtttggca
      661 cgcagcagcc ggaacgagta catgcgctcg ggcctctatt ccaccttcac catccagtct
      721 ctgcagccgc agccaccccc tcatcctcgg cctgctgtgg tgctccccaa tgcagctcct
      781 gcaggggcag cagcgccccc ctcggggagc aggagcacca gtccaagccc cttggaggcc
      841 tgtctggagg ctgaagaggc cggcttgcct ctgcaggtca tcctgacccc gcccga
//
