LOCUS       sequence-33470-e         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For mammalian expression of human Elk-1 with an HA tag and a 
            mutation in the Ets DNA binding domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Lee SM, Vasishtha M, Prywes R
  TITLE     Activation and repression of cellular immediate early genes by serum
            response factor cofactors.
  JOURNAL   J Biol Chem. 2010 Jul 16. 285(29):22036-49.
  PUBMED    20466732
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2010 Jul 16. 285(29):22036-49."
COMMENT     Sequence Label: pCGN-ELK-1 Ets mut
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     5'UTR           84..123
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             153..179
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             159..185
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
       61 cgggaccgat ccagcctccg ggggatcttg gtggcgtgaa actcccgcac ctcttcggcc
      121 agcgccttgt agaagcgcgt atggcttcta gctatcctta tgacgtgcct gactatgcca
      181 gcctgggagg accttctaga atggacccat ctgtgacgct gtggcagttt ctgctgcagc
      241 tgctgagaga gcaaggcaat ggccacatca tctcctggac ttcacgggat ggtggtgaat
      301 tcaagctggt ggatgcagag gaggtggccc ggctgtgggg actacgcaag aacaagacca
      361 acatgaatta cgacaagctc agcgaggcct tggaggacta ctatgacaag aacatcatcc
      421 gcaaggtgag cggccagaag ttcgtctaca agtttgtgtc ctaccctgag gtcgcagggt
      481 gctccactga ggactgcccg ccccagccag aggtgtctgt tacctccacc atgccaaatg
      541 tggcccctgc tgctatacat gccgccccag gggacactgt ctctggaaag ccaggcacac
      601 ccaagggtgc aggaatggca ggcccaggcg gtttggcacg cagcagccgg aacgagtaca
      661 tgcgctcggg cctctattcc accttcacca tccagtctct gcagccgcag ccaccccctc
      721 atcctcggcc tgctgtggtg ctccccaatg cagctcctgc aggggcagca gcgcccccct
      781 cggggagcag gagcaccagt ccaagcccct tgga
//
