LOCUS       sequence-334717-        1022 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mCherry-TOSI (TOr-Signal-Indicator) is a fluorescent reporter for 
            mTORC1 signaling (monitoring PDCD4 degradation) which can be 
            introduced to mammalian cells by retrovirus vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Oki T, Mercier F, Kato H, Jung Y, McDonald TO, Spencer JA, Mazzola 
            MC, van Gastel N, Lin CP, Michor F, Kitamura T, Scadden DT
  TITLE     Imaging dynamic mTORC1 pathway activity in vivo reveals marked 
            shifts that support time-specific inhibitor therapy in AML.
  JOURNAL   Nat Commun. 2021 Jan 11;12(1):245. doi: 10.1038/s41467-020-20491-8.
  PUBMED    33431855
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-01-11"; volume: "12(1):245. doi"; pages: " 
            10.1038/s41467-020-20491-8"
COMMENT     Sequence Label: pMXs-IP-mCherry-TOSI
FEATURES             Location/Qualifiers
     source          1..1022
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..714
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(152..170)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     420..439
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             745..765
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ggatccatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc
       61 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
      121 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc
      181 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg
      241 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg
      301 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg
      361 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc
      421 cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag
      481 gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac
      541 gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac
      601 aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag
      661 tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta caagggatat
      721 ccatcacact ggcggccgga attgccaaaa aagaagagaa aggtagaatt catggatata
      781 gaaaatgagc agacactgaa tgtgaacccc actgaccctg acaatttaag cgactctctc
      841 ttttctggag atgaagaaaa tgctggcact gaagaaataa agaatgaaat aaatggaaat
      901 tggatttccg catctactat taatgaagct agaatcaatg caaaagccaa aagacgactg
      961 cggaaaaatt catcccggga ctctggccga ggagactcag tcagtgacaa ttgagcggcc
     1021 gc
//
